Results 161 to 170 of about 1,318,238 (218)

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Detection of MEN1 resistance mutations in cell-free DNA from acute leukemia patients treated with menin inhibitors. [PDF]

Blood Cancer J
Shukla N, Charalambous K, Li S, Ko KH, Casanova H, Buehler E, Cobbs C, Patel R, Brannon AR, Ewalt MD, Sulis ML, Carroll WL, Arcila ME, Mohibullah N, Berger M, Levine RL, Stein EM, Shah R, Cai SF.   +18 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Beyond a Biomarker: Investigations of a Proinflammatory Role for Cell-Free DNA in Liver Transplant Ischemia and Reperfusion. [PDF]

Transpl Int
Schnepppfister M, Wang Y, Zhong C, Huey T, El-Shewy H, Kao Y, Scalea JR, Morinelli TA, Zhai Y, van der Windt DJ.   +9 more
europepmc   +1 more source

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

FEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento, Danniel Pereira‐Figueiredo, Gabriella Grossi de Lima Dias, Gabriel Ferreira dos Santos, Mariana Rodrigues Pereira, Rafael Brito, Karin C Calaza   +6 more
wiley   +1 more source

Early prediction of gestational diabetes mellitus with clinical characteristics, cell-free DNA and genetic variants. [PDF]

J Transl Med
Chen S, Wang L, Zhu Q, Wang F, Tang C, Zhang B, Shi Y, Liu M, Liu C, Wang Y, Cao X, Cheng M, Peng M, Yuan Y, Kong L, Liang B, Shi Z, Huang H, Xu C, Wu Y.   +19 more
europepmc   +1 more source

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

FEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey, Vaishnavi Sanjayan, Praveen Singh, Shantanu Sengupta, Anand Kumar Bachhawat   +4 more
wiley   +1 more source

Cell-free DNA fragmentomes for noninvasive detection of liver cirrhosis and other diseases. [PDF]

Sci Transl Med
Annapragada AV, Foda ZH, Orjuela H, Norton C, Koul S, Niknafs N, Short S, Boyapati K, Bartolomucci A, Mathios D, Noë M, Cherry C, Carey J, Leal A, Chesnick B, Dracopoli NC, Medina JE, Vulpescu NA, Bruhm DC, Bacus S, Adleff V, Kim AK, Baylin SB, Kirk GD, Sorop A, Iacob R, Iacob S, Gheorghe L, Dima S, Ramírez-Zea M, McGlynn KA, Feltoft CL, Johansen JS, Groopman J, Phallen J, Scharpf RB, Velculescu VE.   +36 more
europepmc   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source