Results 11 to 20 of about 4,735,099 (379)

Evaluation of Cell-Based and Surrogate SARS-CoV-2 Neutralization Assays

Journal of Clinical Microbiology, 2021
Determinants of protective immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection require the development of well-standardized, reproducible antibody assays.
A. Sholukh, Andrew J Fiore-Gartland, E. S. Ford, Maurine D. Miner, Yixuan J. Hou, Longping V. Tse, Hannah Kaiser, Haiying Zhu, Joyce Y C Lu, B. Madarampalli, A. Park, Florian A. Lempp, Russell St. Germain, Emily L Bossard, J. J. Kee, K. Diem, A. Stuart, P. Rupert, C. Brock, Matthew Buerger, M. Doll, A. Randhawa, L. Stamatatos, R. Strong, Colleen C McLaughlin, Meei-Li W Huang, K. Jerome, R. Baric, D. Montefiori, L. Corey   +29 more
semanticscholar   +3 more sources

A chemical susceptibility profile of the Plasmodium falciparum transmission stages by complementary cell-based gametocyte assays. [PDF]

Journal of Antimicrobial Chemotherapy, 2016
S. D’Alessandro, G. Camarda, Y. Corbett, Giulia Siciliano, S. Parapini, L. Cevenini, E. Michelini, A. Roda, D. Leroy, D. Taramelli, P. Alano   +10 more
semanticscholar   +2 more sources

Monitoring fusion kinetics of viral and target cell membranes in living cells using a SARS-CoV-2 spike-protein-mediated membrane fusion assay

STAR Protocols, 2022
Summary: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein mediates membrane fusion between the virus and the target cells, triggering viral entry into the latter.
Hesham Nasser, Ryo Shimizu, Jumpei Ito, Akatsuki Saito, Kei Sato, Terumasa Ikeda, Keita Matsuno, Naganori Nao, Hirofumi Sawa, Mai Kishimoto, Shinya Tanaka, Masumi Tsuda, Lei Wang, Yoshikata Oda, Marie Kato, Zannatul Ferdous, Hiromi Mouri, Kenji Shishido, Takasuke Fukuhara, Tomokazu Tamura, Rigel Suzuki, Hayato Ito, Daichi Yamasoba, Izumi Kimura, Naoko Misawa, Keiya Uriu, Yusuke Kosugi, Shigeru Fujita, Mai Suganami, Mika Chiba, Ryo Yoshimura, So Nakagawa, Jiaqi Wu, Akifumi Takaori-Kondo, Kotaro Shirakawa, Kayoko Nagata, Yasuhiro Kazuma, Ryosuke Nomura, Yoshihito Horisawa, Yusuke Tashiro, Yugo Kawai, Takashi Irie, Ryoko Kawabata, MST Monira Begum, Otowa Takahashi, Kimiko Ichihara, Takamasa Ueno, Chihiro Motozono, Mako Toyoda, Yuri L. Tanaka, Erika P. Butlertanaka, Maya Shofa, Kazuo Takayama, Rina Hashimoto, Sayaka Deguchi, Takao Hashiguchi, Tateki Suzuki, Kanako Kimura, Jiei Sasaki, Yukari Nakajima, Kaori Tabata   +60 more
doaj   +1 more source

Protocol for the determination of intracellular phase separation thresholds

STAR Protocols, 2021
Summary: To date, phase separation studies have largely been limited to in vitro assays using non-native conditions and aggregation-prone recombinant proteins that are often difficult to purify.
Anna K. Lee, Patrick Ryan Potts
doaj   +1 more source

Analysis of mannosidase I activity in interphase and mitotic cells by lectin staining and endoglycosidase H treatment

STAR Protocols, 2023
Summary: N-Glycosylation is a common protein modification catalyzed by a series of glycosylation enzymes in the endoplasmic reticulum and Golgi apparatus.
Jie Li, Jianchao Zhang, Yanzhuang Wang
doaj   +1 more source

Detection of DNA damage by alkaline comet assay in mouse colonic mucosa

STAR Protocols, 2021
Summary: We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin.
Elisa Boutet-Robinet, Maria M. Haykal, Saleha Hashim, Teresa Frisan, Océane C.B. Martin   +4 more
doaj   +1 more source

Protocol to measure inter-centrosome distance in adherent cells using epifluorescence microscopy

STAR Protocols, 2022
Summary: We present here a protocol to assay the centrosome separation events at late-G2 phase of the cell cycle by immunofluorescence microscopy. We describe the steps required for imaging and measurement of inter-centrosome distance.
Franco K.C. Au, Edna S.W. Lui, Robert Z. Qi   +2 more
doaj   +1 more source

Multiplex Profiling of Cellular Invasion in 3D Cell Culture Models. [PDF]

, 2012
To-date, most invasion or migration assays use a modified Boyden chamber-like design to assess migration as single-cell or scratch assays on coated or uncoated planar plastic surfaces.
Burgstaller, Gerald, Eickelberg, Oliver, Koch, Ina, Lindner, Michael, Oehrle, Bettina   +4 more
core   +6 more sources

Measuring SERCA-mediated calcium uptake in mouse muscle homogenates

STAR Protocols, 2023
Summary: This protocol employs the indo-1 Ca2+ fluorophore to quantify Ca2+ uptake by the sarco(endo)plasmic reticulum Ca2+ ATPase pump in murine muscle homogenates and allows for real-time kinetic measurement of Ca2+ mobilization within the muscle ...
Mia S. Geromella, Jessica L. Braun, Val A. Fajardo   +2 more
doaj   +1 more source

A luminescence-based protocol for assessing fructose metabolism via quantification of ketohexokinase enzymatic activity in mouse or human hepatocytes

STAR Protocols, 2021
Summary: Ketohexokinase (KHK) catalyzes the first step of fructose metabolism. Inhibitors of KHK enzymatic activity are being evaluated in clinical trials for the treatment of non-alcoholic fatty liver disease (NAFLD) and diabetes.
Se-Hyung Park, Robert N. Helsley, Leila Noetzli, Ho-Chou Tu, Kristina Wallenius, Gavin O’Mahony, Jeremie Boucher, Jianming Liu, Samir Softic   +8 more
doaj   +1 more source