Results 11 to 20 of about 1,330,091 (168)
Protocol to measure inter-centrosome distance in adherent cells using epifluorescence microscopy
STAR Protocols, 2022 Summary: We present here a protocol to assay the centrosome separation events at late-G2 phase of the cell cycle by immunofluorescence microscopy. We describe the steps required for imaging and measurement of inter-centrosome distance.
Franco K.C. Au +2 more
doaj +1 more source
Measuring SERCA-mediated calcium uptake in mouse muscle homogenates
STAR Protocols, 2023 Summary: This protocol employs the indo-1 Ca2+ fluorophore to quantify Ca2+ uptake by the sarco(endo)plasmic reticulum Ca2+ ATPase pump in murine muscle homogenates and allows for real-time kinetic measurement of Ca2+ mobilization within the muscle ...
Mia S. Geromella +2 more
doaj +1 more source
STAR Protocols, 2021 Summary: Ketohexokinase (KHK) catalyzes the first step of fructose metabolism. Inhibitors of KHK enzymatic activity are being evaluated in clinical trials for the treatment of non-alcoholic fatty liver disease (NAFLD) and diabetes.
Se-Hyung Park +8 more
doaj +1 more source
Measurement of autophagy via LC3 western blotting following DNA-damage-induced senescence
STAR Protocols, 2022 Summary: Senescent cells accumulation is associated with aging and age-related diseases, and recent findings suggest that autophagy, the activity of the intracellular degradation system, decreases during senescence.
Hitomi Yamamoto-Imoto +3 more
doaj +1 more source
Protocol for single-molecule pull-down of fluorescently tagged oligomers from cell lysates
STAR Protocols, 2023 Summary: Mutations in intrinsically disordered proteins drive the irreversible formation of pathological aggregates, a hallmark of neurodegenerative diseases.
Nathalie Djaja, Sua Myong
doaj +1 more source
STAR Protocols, 2023 Summary: Here, we present a protocol for assessing metabolic activity of bacterial populations by measuring heat flow using isothermal calorimetry. We outline the steps for preparing the different growth models of Pseudomonas aeruginosa and performing ...
Katrin Beilharz +6 more
doaj +1 more source
Protocol for imaging proteins associated with Legionella-containing vacuoles in host cells
STAR Protocols, 2021 Summary: The intracellular bacterial pathogen Legionella pneumophila exploits host cellular systems using approximately 300 effector proteins to establish a replicative niche known as the Legionella-containing vacuole (LCV).
Tomoe Kitao +3 more
doaj +1 more source
Microfluidic System for Automated Cell-Based Assays [PDF]
JALA: Journal of the Association for Laboratory Automation, 2007 Microfluidic cell culture is a promising technology for applications in the drug screening industry. Key benefits include improved biological function, higher-quality cell-based data, reduced reagent consumption, and lower cost. In this work, we demonstrate how a microfluidic cell culture design was adapted to be compatible with the standard 96-well ...
Philip J, Lee +3 more
openaire +2 more sources
Titration and neutralizing antibody quantification by focus forming assay for Powassan virus
STAR Protocols, 2022 Summary: The development of high-throughput assays measuring Powassan virus (POWV) lineage I and II represents an important step in virological and immunological studies.
E. Taylor Stone +4 more
doaj +1 more source
Fluorescence-based protocol for revealing cellular arrangement in biofilms
STAR Protocols, 2023 Summary: Standardized assays have greatly advanced the understanding of multicellular bacterial biofilms, but they lack cell-scale detail. Here, we present a fluorescence-based protocol that builds on past assays to reveal the cellular-scale arrangement ...
Devina Puri, Xin Fang, Kyle R. Allison
doaj +1 more source