Results 21 to 30 of about 1,330,091 (168)

Relationship Between the Salivary Microbiome and Oral Malodor Metabolites in Older Thai Individuals with Periodontitis and the Cytotoxic Effects of Malodor Compounds on Human Oral Squamous Carcinoma (HSC-4) Cells

Dentistry Journal
Background/Objectives: Halitosis is primarily caused by the activity of oral microorganisms. In this study, we employed metagenomic sequencing and metabolomic approaches to investigate the differences in salivary microbiota and metabolite profiles ...
Witsanu Srila, Kritsana Sripilai, Thunwa Binlateh, Peungchaleoy Thammanichanon, Watcharaphol Tiskratok, Parinya Noisa, Paiboon Jitprasertwong   +6 more
doaj   +1 more source

A tissue culture infectious dose-derived protocol for testing of SARS-CoV-2 neutralization of serum antibodies on adherent cells

STAR Protocols, 2021
Summary: For a cytopathic virus such as severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), the neutralization capacity of serum from convalescent or vaccinated persons or of therapeutic antibodies can be tested on adherent cell cultures ...
Fabio Hasler, Agathe Duda, Thomas M. Kündig, Pål Johansen   +3 more
doaj   +1 more source

Live-cell metabolic analysis of oligodendroglia isolated from postnatal mouse brain and spinal cord

STAR Protocols, 2022
Summary: This protocol describes isolation and live-cell metabolic analysis of O4+ oligodendroglia from brain and spinal cord of postnatal mice. We have optimized existing protocols for O4+ isolation from neonatal brain and expanded the protocol to ...
Luipa Khandker, Teresa L. Wood
doaj   +1 more source

Microfluidics for cell-based assays

Materials Today, 2005
Microfluidic systems are powerful tools in chemistry, physics, and biology. The behavior of confined fluids differs from macroscopic systems and allows precise control of the chemical composition of fluids, as well as the temporal and spatial patterns of microscopic fluid elements.
Pihl, Johan, Sinclair, Jon, Karlsson, Mattias, Orwar, Owe   +3 more
openaire   +1 more source

Cell-Based Assays for Screening Androgen Receptor Ligands [PDF]

Seminars in Reproductive Medicine, 2015
The androgen receptor (AR, NR3C4) mediates the majority of androgen effects on target cells. The AR is activated following ligand binding that result is enhanced of target gene transcription. Several cell-based model systems have been developed that allow sensitive detection and monitoring of steroids or other compounds with AR bioactivity.
Campana C, PEZZI, Vincenzo, Rainey WE
openaire   +3 more sources

3D culture of alginate-hyaluronic acid hydrogel supports the stemness of human mesenchymal stem cells

Scientific Reports
The three-dimensional (3D) cell culture system is being employed more frequently to investigate cell engineering and tissue repair due to its close mimicry of in vivo microenvironments.
Amorn Pangjantuk, Palakorn Kaokaen, Phongsakorn Kunhorm, Nipha Chaicharoenaudomrung, Parinya Noisa   +4 more
doaj   +1 more source

Adverse outcome pathway-based analysis of liver steatosis in vitro using human liver cell lines

STAR Protocols, 2023
Summary: Here, we present an in vitro test battery to analyze chemicals for their potential to induce liver triglyceride accumulation, a hallmark of liver steatosis.
Mawien Karaca, Kristin Fritsche, Dajana Lichtenstein, Özlem Vural, Katrin Kreuzer, Jimmy Alarcan, Albert Braeuning, Philip Marx-Stoelting, Tewes Tralau   +8 more
doaj   +1 more source

Measuring CPT-1-mediated respiration in permeabilized cells and isolated mitochondria

STAR Protocols, 2021
Summary: Carnitine palmitoyltransferase-1 (CPT-1) is a rate-controlling enzyme for long-chain fatty acid oxidation. This manuscript provides protocols for measuring CPT-1-mediated respiration in permeabilized, adherent cell monolayers and mitochondria ...
Krista Yang, Mary T. Doan, Linsey Stiles, Ajit S. Divakaruni   +3 more
doaj   +1 more source

Protocol for in vitro assessment of human monocyte transendothelial migration using a high-throughput live cell imaging system

STAR Protocols, 2023
Summary: In vitro modeling of the different steps of immune cell recruitment is essential to decipher the role of endothelial cells in this process. Here, we present a protocol for the assessment of human monocyte transendothelial migration using a live ...
Ségolène Ladaigue, Vincent Paget, Anne-Charlotte Lefranc, Monica Quitoco, Emilie Bacquer, Fabien Milliat, Olivier Guipaud   +6 more
doaj   +1 more source

Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures

STAR Protocols
Summary: The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures.
Stina Oredsson, Atena Malakpour-Permlid, Johan Zhu, Tilo Weber   +3 more
doaj   +1 more source