Protocol for imaging proteins associated with Legionella-containing vacuoles in host cells
STAR Protocols, 2021 Summary: The intracellular bacterial pathogen Legionella pneumophila exploits host cellular systems using approximately 300 effector proteins to establish a replicative niche known as the Legionella-containing vacuole (LCV).
Tomoe Kitao +3 more
doaj +1 more source
Sensing Cell-Culture Assays with Low-Cost Circuitry [PDF]
, 2018 An alternative approach for cell-culture end-point protocols is proposed herein. This new technique is suitable for real-time remote sensing. It is based on Electrical Cell-substrate Impedance Spectroscopy (ECIS) and employs the Oscillation-Based Test ...
Daza Navarro, María Paula +7 more
core +1 more source
Titration and neutralizing antibody quantification by focus forming assay for Powassan virus
STAR Protocols, 2022 Summary: The development of high-throughput assays measuring Powassan virus (POWV) lineage I and II represents an important step in virological and immunological studies.
E. Taylor Stone +4 more
doaj +1 more source
Diagnostic assays for leprosy based on T-cell epitopes. [PDF]
, 2000 To date, only a limited number of antigens have been described as specific for Mycobacterium leprae, and in many cases, homologues have subsequently been shown to exist in mycobacteria such as M. avium and M. intracellulare.
Brahmbhatt, S +16 more
core +1 more source
A Monolithically Fabricated Combinatorial Mixer for Microchip-Based High-Throughput Cell Culturing Assays [PDF]
, 2007 We present an integrated method to fabricate 3- D microfluidic networks and fabricated the first on-chip cell culture device with an integrated combinatorial mixer.
Ho, Dean, Liu, Mike C., Tai, Yu-Chong
core +1 more source
Fluorescence-based protocol for revealing cellular arrangement in biofilms
STAR Protocols, 2023 Summary: Standardized assays have greatly advanced the understanding of multicellular bacterial biofilms, but they lack cell-scale detail. Here, we present a fluorescence-based protocol that builds on past assays to reveal the cellular-scale arrangement ...
Devina Puri, Xin Fang, Kyle R. Allison
doaj +1 more source
Identification of a 3-Alkylpyridinium Compound from the Red Sea Sponge Amphimedon chloros with In Vitro Inhibitory Activity against the West Nile Virus NS3 Protease. [PDF]
, 2018 Viruses are underrepresented as targets in pharmacological screening efforts, given the difficulties of devising suitable cell-based and biochemical assays.
Duggan, Brendan M +8 more
core +3 more sources
A–C Estrogens as Potent and Selective Estrogen Receptor-Beta Agonists (SERBAs) to Enhance Memory Consolidation under Low-Estrogen Conditions [PDF]
, 2018 Estrogen receptor-beta (ERβ) is a drug target for memory consolidation in postmenopausal women. Herein is reported a series of potent and selective ERβ agonists (SERBAs) with in vivo efficacy that are A–C estrogens, lacking the B and D estrogen rings ...
Donaldson, William A. +12 more
core +4 more sources
STAR Protocols, 2021 Summary: For a cytopathic virus such as severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), the neutralization capacity of serum from convalescent or vaccinated persons or of therapeutic antibodies can be tested on adherent cell cultures ...
Fabio Hasler +3 more
doaj +1 more source
Dentistry JournalBackground/Objectives: Halitosis is primarily caused by the activity of oral microorganisms. In this study, we employed metagenomic sequencing and metabolomic approaches to investigate the differences in salivary microbiota and metabolite profiles ...
Witsanu Srila +6 more
doaj +1 more source