Results 21 to 30 of about 4,481,323 (329)

Protocol for the determination of intracellular phase separation thresholds

STAR Protocols, 2021
Summary: To date, phase separation studies have largely been limited to in vitro assays using non-native conditions and aggregation-prone recombinant proteins that are often difficult to purify.
Anna K. Lee, Patrick Ryan Potts
doaj  

Comparison of cell-based assays to quantify treatment effects of anticancer drugs identifies a new application for Bodipy-L-cystine to measure apoptosis

Scientific Reports, 2018
Cell-based assays that measure anticancer drug effects are essential for evaluating chemotherapeutic agents. Many assays targeting various cellular mechanisms are available, leading to inconsistent results when using different techniques.
Nita Kumar, Rayhaneh Afjei, T. Massoud, R. Paulmurugan   +3 more
semanticscholar   +1 more source

Evaluation of Cell-Based and Surrogate SARS-CoV-2 Neutralization Assays

Journal of Clinical Microbiology, 2021
Determinants of protective immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection require the development of well-standardized, reproducible antibody assays.
A. Sholukh, Andrew J Fiore-Gartland, E. Ford, Maurine D. Miner, Yixuan J. Hou, Longping V. Tse, Hannah Kaiser, Haiying Zhu, Joyce Y C Lu, B. Madarampalli, A. Park, Florian A. Lempp, Russell St. Germain, Emily L Bossard, J. J. Kee, K. Diem, A. Stuart, P. Rupert, C. Brock, Matthew Buerger, M. Doll, A. Randhawa, L. Stamatatos, R. Strong, Colleen C McLaughlin, Meei-Li W Huang, K. Jerome, R. Baric, D. Montefiori, L. Corey   +29 more
semanticscholar   +1 more source

Monitoring fusion kinetics of viral and target cell membranes in living cells using a SARS-CoV-2 spike-protein-mediated membrane fusion assay

STAR Protocols, 2022
Summary: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein mediates membrane fusion between the virus and the target cells, triggering viral entry into the latter.
Hesham Nasser, Ryo Shimizu, Jumpei Ito, Akatsuki Saito, Kei Sato, Terumasa Ikeda, Keita Matsuno, Naganori Nao, Hirofumi Sawa, Mai Kishimoto, Shinya Tanaka, Masumi Tsuda, Lei Wang, Yoshikata Oda, Marie Kato, Zannatul Ferdous, Hiromi Mouri, Kenji Shishido, Takasuke Fukuhara, Tomokazu Tamura, Rigel Suzuki, Hayato Ito, Daichi Yamasoba, Izumi Kimura, Naoko Misawa, Keiya Uriu, Yusuke Kosugi, Shigeru Fujita, Mai Suganami, Mika Chiba, Ryo Yoshimura, So Nakagawa, Jiaqi Wu, Akifumi Takaori-Kondo, Kotaro Shirakawa, Kayoko Nagata, Yasuhiro Kazuma, Ryosuke Nomura, Yoshihito Horisawa, Yusuke Tashiro, Yugo Kawai, Takashi Irie, Ryoko Kawabata, MST Monira Begum, Otowa Takahashi, Kimiko Ichihara, Takamasa Ueno, Chihiro Motozono, Mako Toyoda, Yuri L. Tanaka, Erika P. Butlertanaka, Maya Shofa, Kazuo Takayama, Rina Hashimoto, Sayaka Deguchi, Takao Hashiguchi, Tateki Suzuki, Kanako Kimura, Jiei Sasaki, Yukari Nakajima, Kaori Tabata   +60 more
doaj  

Protocol for assessing ex vivo lipolysis of murine adipose tissue

STAR Protocols, 2022
Summary: Here, we provide a detailed protocol for assessing ex vivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic ...
Debasish Roy, Julia M. Myers, Andrea Tedeschi   +2 more
doaj  

Testing therapeutics in cell-based assays: Factors that influence the apparent potency of drugs

PLoS ONE, 2018
Identifying effective antivirals for treating Ebola virus disease (EVD) and minimizing transmission of such disease is critical. A variety of cell-based assays have been developed for evaluating compounds for activity against Ebola virus.
E. Postnikova, Yu Cong, Lisa Evans DeWald, J. Dyall, Shuǐqìng Yú, Brit J. Hart, Huanying Zhou, R. Gross, James Logue, Yíngyún Caì, Nicole M Deiuliis, J. Michelotti, A. Honko, R. S. Bennett, M. Holbrook, G. Olinger, L. Hensley, P. Jahrling   +17 more
semanticscholar   +1 more source

Analysis of mannosidase I activity in interphase and mitotic cells by lectin staining and endoglycosidase H treatment

STAR Protocols, 2023
Summary: N-Glycosylation is a common protein modification catalyzed by a series of glycosylation enzymes in the endoplasmic reticulum and Golgi apparatus.
Jie Li, Jianchao Zhang, Yanzhuang Wang
doaj  

Quantitative Cell-based Protein Degradation Assays to Identify and Classify Drugs That Target the Ubiquitin-Proteasome System [PDF]

, 2011
We have generated a set of dual-reporter human cell lines and devised a chase protocol to quantify proteasomal degradation of a ubiquitin fusion degradation (UFD) substrate, a ubiquitin ligase CRL2^(VHL) substrate, and a ubiquitin-independent substrate ...
Aghajan, Alvarez-Castelao, Bence, Beskow, Boyault, Boyault, Cao, Chou, Colson, Dantuma, DeLaBarre, Deshaies, Ding, Eldridge, Elofsson, Finley, Giaever, Golbik, Ito, Janiesch, Ju, Kane, Kimbrel, Lee, Lois, Milhazes, Müller, Nawrocki, Neuber, Noguchi, Ohh, Patel, Rabouille, Raymond J. Deshaies, Richly, Schrader, Soucy, Tsui-Fen Chou, Tsujimoto, Wang, Wang, Weissman, Yamamoto, Yamamoto, Yamamoto, Yang, Ye, Ye, Ye, Zhang, Zhang   +50 more
core   +3 more sources

2018 White Paper on Recent Issues in Bioanalysis: focus on flow cytometry, gene therapy, cut points and key clarifications on BAV (Part 3 - LBA/cell-based assays: immunogenicity, biomarkers and PK assays).

Bioanalysis, 2018
The 2018 12th Workshop on Recent Issues in Bioanalysis took place in Philadelphia, PA, USA on April 9-13, 2018 with an attendance of over 900 representatives from pharmaceutical/biopharmaceutical companies, biotechnology companies, contract research ...
Lauren F. Stevenson, S. Richards, R. Pillutla, A. Torri, John Kamerud, D. Mehta, Stephen Keller, S. Purushothama, B. Gorovits, V. Litwin, C. Stebbins, J. Marini, Chris Beaver, Gizette V. Sperinde, Patricia Siguenza, R. Staack, Y. Qiu, L. Amaravadi, Shashi Amur, C. Fleener, D. Baltrukonis, Ian M. Catlett, E. Cherry, Shan Chung, I. Cludts, L. D. Donato, S. Fischer, S. Fraser, F. Garofolo, Cherie Green, G. Gunn, Sam H. Haidar, Jonathan Haulenbeek, Neil Henderson, Shirley Hopper, A. Ishii-Watabe, Rafiq Islam, B. Janelsins, V. Jawa, V. Kakkanaiah, Szilárd Kamondi, G. Kolaitis, R. Kubiak, Seema C Kumar, P. Kurki, M. Liang, Patrick M Liu, K. Maxfield, H. Myler, N. Palackal, R. Palmer, J. Pedras-Vasconcelos, S. Piccoli, P. Rhyne, Yoshiro Saito, Natasha Savoie, Eginhard Schick, B. Schweighardt, Judy Y Shih, A. Song, P. Sriraman, L. Staelens, G. Sumner, Yongliang Sun, M. Ullmann, D. Verthelyi, M. Wadhwa, Yow-Ming C Wang, Yuanxin Xu, Haoheng Yan, Tong-yuan Yang, Rong Zeng   +71 more
semanticscholar   +1 more source

CD171- and GD2-specific CAR-T cells potently target retinoblastoma cells in preclinical in vitro testing [PDF]

, 2019
BACKGROUND: Chimeric antigen receptor (CAR)-based T cell therapy is in early clinical trials to target the neuroectodermal tumor, neuroblastoma. No preclinical or clinical efficacy data are available for retinoblastoma to date.
Andersch, Lena, Eggert, Angelika, Flemmig, Carina, Grunewald, Laura, Henssen, Anton, Jensen, Michael C., Joussen, Antonia, Klaus, Anika, Künkele, Annette, Radke, Josefine, Rossig, Claudia, Schulte, Johannes H., Schumann, Elisa, Schwiebert, Silke, Winkler, Annika, Zirngibl, Felix   +15 more
core   +2 more sources