A Morphological identification cell cytotoxicity assay using cytoplasm-localized fluorescent probe (CLFP) to distinguish living and dead cells [PDF]
, 2016 Cell cytotoxicity assays include cell activity assays and morphological identification assays. Currently, all frequently used cytotoxicity assays belong to cell activity assays but suffer from detection limitations.
Chen, Jialing +8 more
core +2 more sources
Structural optimization and biological evaluation of 2-substituted 5-hydroxyindole-3-carboxylates as potent inhibitors of human 5-lipoxygenase. [PDF]
, 2009 Pharmacological suppression of leukotriene biosynthesis by inhibitors of 5-lipoxygenase (5-LO) is a strategy to intervene with inflammatory and allergic disorders.
BÜHRING U +8 more
core +1 more source
Diagnostic assays for leprosy based on T-cell epitopes. [PDF]
, 2000 To date, only a limited number of antigens have been described as specific for Mycobacterium leprae, and in many cases, homologues have subsequently been shown to exist in mycobacteria such as M. avium and M. intracellulare.
Brahmbhatt, S +16 more
core +1 more source
Characterization of small molecules inhibiting the pro-angiogenic activity of the zinc finger transcription factor Vezf1 [PDF]
, 2018 Discovery of inhibitors for endothelial-related transcription factors can contribute to the development of anti-angiogenic therapies that treat various diseases, including cancer.
Gowher, Humaira +4 more
core +2 more sources
Titration and neutralizing antibody quantification by focus forming assay for Powassan virus
STAR Protocols, 2022 Summary: The development of high-throughput assays measuring Powassan virus (POWV) lineage I and II represents an important step in virological and immunological studies.
E. Taylor Stone +4 more
doaj +1 more source
Protocol for imaging proteins associated with Legionella-containing vacuoles in host cells
STAR Protocols, 2021 Summary: The intracellular bacterial pathogen Legionella pneumophila exploits host cellular systems using approximately 300 effector proteins to establish a replicative niche known as the Legionella-containing vacuole (LCV).
Tomoe Kitao +3 more
doaj +1 more source
Microfluidic System for Automated Cell-Based Assays [PDF]
JALA: Journal of the Association for Laboratory Automation, 2007 Microfluidic cell culture is a promising technology for applications in the drug screening industry. Key benefits include improved biological function, higher-quality cell-based data, reduced reagent consumption, and lower cost. In this work, we demonstrate how a microfluidic cell culture design was adapted to be compatible with the standard 96-well ...
Philip J, Lee +3 more
openaire +2 more sources
Cell wall composition and biofilm formation of azoles-susceptible and -resistant Candida glabrata strains [PDF]
, 2011 In the present study, three strains of Candida glabrata have been investigated to shed light on the mechanisms involved in azole resistance during adherence and biofilm formation.
Vitali, Alberto +6 more
core +5 more sources
Fluorescence-based protocol for revealing cellular arrangement in biofilms
STAR Protocols, 2023 Summary: Standardized assays have greatly advanced the understanding of multicellular bacterial biofilms, but they lack cell-scale detail. Here, we present a fluorescence-based protocol that builds on past assays to reveal the cellular-scale arrangement ...
Devina Puri, Xin Fang, Kyle R. Allison
doaj +1 more source
A Monolithically Fabricated Combinatorial Mixer for Microchip-Based High-Throughput Cell Culturing Assays [PDF]
, 2007 We present an integrated method to fabricate 3- D microfluidic networks and fabricated the first on-chip cell culture device with an integrated combinatorial mixer.
Ho, Dean, Liu, Mike C., Tai, Yu-Chong
core +1 more source