Measurement of autophagy via LC3 western blotting following DNA-damage-induced senescence
STAR Protocols, 2022 Summary: Senescent cells accumulation is associated with aging and age-related diseases, and recent findings suggest that autophagy, the activity of the intracellular degradation system, decreases during senescence.
Hitomi Yamamoto-Imoto +3 more
doaj +1 more source
Diamagnetic repulsion of particles for multilaminar flow assays [PDF]
, 2015 © The Royal Society of Chemistry. We demonstrate diamagnetic repulsion forces for performing continuous multilaminar flow assays on particles based on their intrinsic properties and with a simple setup. The platform could be applied to sandwich assays on
Elders, Luke T. +3 more
core +1 more source
A multicenter comparison of MOG-IgG cell-based assays
Neurology, 2019 Objectives To compares 3 different myelin oligodendrocyte glycoprotein–immunoglobulin G (IgG) cell-based assays (CBAs) from 3 international centers.
P. Waters +11 more
semanticscholar +1 more source
Protocol for single-molecule pull-down of fluorescently tagged oligomers from cell lysates
STAR Protocols, 2023 Summary: Mutations in intrinsically disordered proteins drive the irreversible formation of pathological aggregates, a hallmark of neurodegenerative diseases.
Nathalie Djaja, Sua Myong
doaj +1 more source
STAR Protocols, 2023 Summary: Here, we present a protocol for assessing metabolic activity of bacterial populations by measuring heat flow using isothermal calorimetry. We outline the steps for preparing the different growth models of Pseudomonas aeruginosa and performing ...
Katrin Beilharz +6 more
doaj +1 more source
Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future [PDF]
, 2014 Serological techniques commonly used to quantify influenza-specific antibodies include the Haemagglutination Inhibition (HI), Single Radial Haemolysis (SRH) and Virus Neutralization (VN) assays.
Callow +30 more
core +3 more sources
A Morphological identification cell cytotoxicity assay using cytoplasm-localized fluorescent probe (CLFP) to distinguish living and dead cells [PDF]
, 2016 Cell cytotoxicity assays include cell activity assays and morphological identification assays. Currently, all frequently used cytotoxicity assays belong to cell activity assays but suffer from detection limitations.
Chen, Jialing +8 more
core +2 more sources
Characterization of small molecules inhibiting the pro-angiogenic activity of the zinc finger transcription factor Vezf1 [PDF]
, 2018 Discovery of inhibitors for endothelial-related transcription factors can contribute to the development of anti-angiogenic therapies that treat various diseases, including cancer.
Gowher, Humaira +4 more
core +2 more sources
SLAS discovery : advancing life sciences R & D, 2019 The introduction of more relevant cell models in early preclinical drug discovery, combined with high-content imaging and automated analysis, is expected to increase the quality of compounds progressing to preclinical stages in the drug development ...
T. Booij, L. Price, E. Danen
semanticscholar +1 more source
Structural optimization and biological evaluation of 2-substituted 5-hydroxyindole-3-carboxylates as potent inhibitors of human 5-lipoxygenase. [PDF]
, 2009 Pharmacological suppression of leukotriene biosynthesis by inhibitors of 5-lipoxygenase (5-LO) is a strategy to intervene with inflammatory and allergic disorders.
BÜHRING U +8 more
core +1 more source