Sensing Cell-Culture Assays with Low-Cost Circuitry [PDF]
, 2018 An alternative approach for cell-culture end-point protocols is proposed herein. This new technique is suitable for real-time remote sensing. It is based on Electrical Cell-substrate Impedance Spectroscopy (ECIS) and employs the Oscillation-Based Test ...
Daza Navarro, María Paula +7 more
core +1 more source
Multicentre comparison of a diagnostic assay: Aquaporin-4 antibodies in neuromyelitis optica [PDF]
, 2016 Objective Antibodies to cell surface central nervous system proteins help to diagnose conditions which often respond to immunotherapies. The assessment of antibody assays needs to reflect their clinical utility.
Aktas, O. (Orhan) +44 more
core +1 more source
STAR Protocols, 2021 Summary: For a cytopathic virus such as severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), the neutralization capacity of serum from convalescent or vaccinated persons or of therapeutic antibodies can be tested on adherent cell cultures ...
Fabio Hasler +3 more
doaj +1 more source
Identification of a 3-Alkylpyridinium Compound from the Red Sea Sponge Amphimedon chloros with In Vitro Inhibitory Activity against the West Nile Virus NS3 Protease. [PDF]
, 2018 Viruses are underrepresented as targets in pharmacological screening efforts, given the difficulties of devising suitable cell-based and biochemical assays.
Duggan, Brendan M +8 more
core +3 more sources
Live-cell metabolic analysis of oligodendroglia isolated from postnatal mouse brain and spinal cord
STAR Protocols, 2022 Summary: This protocol describes isolation and live-cell metabolic analysis of O4+ oligodendroglia from brain and spinal cord of postnatal mice. We have optimized existing protocols for O4+ isolation from neonatal brain and expanded the protocol to ...
Luipa Khandker, Teresa L. Wood
doaj +1 more source
A–C Estrogens as Potent and Selective Estrogen Receptor-Beta Agonists (SERBAs) to Enhance Memory Consolidation under Low-Estrogen Conditions [PDF]
, 2018 Estrogen receptor-beta (ERβ) is a drug target for memory consolidation in postmenopausal women. Herein is reported a series of potent and selective ERβ agonists (SERBAs) with in vivo efficacy that are A–C estrogens, lacking the B and D estrogen rings ...
Donaldson, William A. +12 more
core +4 more sources
Dentistry JournalBackground/Objectives: Halitosis is primarily caused by the activity of oral microorganisms. In this study, we employed metagenomic sequencing and metabolomic approaches to investigate the differences in salivary microbiota and metabolite profiles ...
Witsanu Srila +6 more
doaj +1 more source
Iterative in Situ Click Chemistry Assembles a Branched Capture Agent and Allosteric Inhibitor for Akt1 [PDF]
, 2011 We describe the use of iterative in situ click chemistry to design an Akt-specific branched peptide triligand that is a drop-in replacement for monoclonal antibodies in multiple biochemical assays.
Agnew, Heather D. +12 more
core +2 more sources
Adverse outcome pathway-based analysis of liver steatosis in vitro using human liver cell lines
STAR Protocols, 2023 Summary: Here, we present an in vitro test battery to analyze chemicals for their potential to induce liver triglyceride accumulation, a hallmark of liver steatosis.
Mawien Karaca +8 more
doaj +1 more source
Microfluidics for cell-based assays
Materials Today, 2005 Microfluidic systems are powerful tools in chemistry, physics, and biology. The behavior of confined fluids differs from macroscopic systems and allows precise control of the chemical composition of fluids, as well as the temporal and spatial patterns of microscopic fluid elements.
Pihl, Johan +3 more
openaire +1 more source