Results 31 to 40 of about 4,481,323 (329)
Detection of DNA damage by alkaline comet assay in mouse colonic mucosa
STAR Protocols, 2021 Summary: We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin.
Elisa Boutet-Robinet +4 more
doaj
Journal of Cell Science, 2018 The human Ras superfamily of small GTPases controls essential cellular processes such as gene expression and cell proliferation. As their deregulation is widely associated with human cancer, small GTPases and their regulatory proteins have become ...
Faten Koraïchi +6 more
semanticscholar +1 more source
The Effects of Statistical Multiplicity of Infection on Virus Quantification and Infectivity Assays
, 2018 Many biological assays are employed in virology to quantify parameters of interest. Two such classes of assays, virus quantification assays (VQA) and infectivity assays (IA), aim to estimate the number of viruses present in a solution, and the ability of
Chou, Tom +2 more
core +1 more source
Dentistry JournalBackground/Objectives: Halitosis is primarily caused by the activity of oral microorganisms. In this study, we employed metagenomic sequencing and metabolomic approaches to investigate the differences in salivary microbiota and metabolite profiles ...
Witsanu Srila +6 more
doaj +1 more source
Protocol to measure inter-centrosome distance in adherent cells using epifluorescence microscopy
STAR Protocols, 2022 Summary: We present here a protocol to assay the centrosome separation events at late-G2 phase of the cell cycle by immunofluorescence microscopy. We describe the steps required for imaging and measurement of inter-centrosome distance.
Franco K.C. Au +2 more
doaj
Measuring SERCA-mediated calcium uptake in mouse muscle homogenates
STAR Protocols, 2023 Summary: This protocol employs the indo-1 Ca2+ fluorophore to quantify Ca2+ uptake by the sarco(endo)plasmic reticulum Ca2+ ATPase pump in murine muscle homogenates and allows for real-time kinetic measurement of Ca2+ mobilization within the muscle ...
Mia S. Geromella +2 more
doaj
Engineered basement membranes: from in vivo considerations to cell-based assays.
Integrative Biology, 2018 Improvements in the physiological relevance of cell-based assays have been enabled by the development of various interdisciplinary methods. However, due to their complexity, in vivo structures such as basement membranes (BMs), which regulate the ...
Guillaume Perry +4 more
semanticscholar +1 more source
A pilot study evaluating concordance between blood-based and patient-matched tumor molecular testing within pancreatic cancer patients participating in the Know Your Tumor (KYT) initiative [PDF]
, 2016 Recent improvements in next-generation sequencing (NGS) technology have enabled detection of biomarkers in cell-free DNA in blood and may ultimately replace invasive tissue biopsies.
Aberra, Metasebia +15 more
core +2 more sources
PLoS ONE, 2017 The chemokine receptor CXCR4 is activated by its unique chemokine ligand CXCL12 and regulates many physiological and developmental processes such as hematopoietic cell trafficking.
A. Van Hout +4 more
semanticscholar +1 more source
The dual nature of TDC – bridging dendritic and T cells in immunity
FEBS Letters, EarlyView.TDC are hematopoietic cells combining dendritic and T cell features. They reach secondary lymphoid organs (SLOs) and peripheral organs (liver and lungs) after FLT3‐dependent development in the bone marrow and maturation in the thymus. TDC are activated and enriched in SLOs upon viral infection, suggesting that they might play unique immune roles, since
Maria Nelli, Mirela Kuka
wiley +1 more source