Results 51 to 60 of about 1,959,228 (334)
STAR Protocols, 2023 Summary: In vitro modeling of the different steps of immune cell recruitment is essential to decipher the role of endothelial cells in this process. Here, we present a protocol for the assessment of human monocyte transendothelial migration using a live ...
Ségolène Ladaigue +6 more
doaj +1 more source
A CMOS Tracking System Approach for Cell Motility Assays [PDF]
, 2017 This work proposes a method for studying and monitoring in real-time a single cell on a 2D electrode matrix, of great interest in cell motility assays and in the characterization of cancer cell metastasis. A CMOS system proposal for cell location based
Huertas Sánchez, Gloria +5 more
core +1 more source
Bone Marrow Failure as an Underrecognized Feature of KAT6A Syndrome
Pediatric Blood &Cancer, EarlyView.ABSTRACT KAT6A syndrome (Arboleda–Tham syndrome) is a rare disorder caused by heterozygous pathogenic variants in KAT6A, a histone acetyltransferase essential for chromatin remodeling and hematopoietic stem cell function. While neurodevelopmental features are well established, hematologic manifestations are underrecognized.
Ye Jee Shim +8 more
wiley +1 more source
STAR Protocols, 2021 Summary: In this protocol, we provide step-by-step instructions for dissection and culture of primary murine dorsal root ganglia (DRG), which provide an opportunity to study the functional properties of peripheral sensory neurons in vitro.
Caroline Perner, Caroline L. Sokol
doaj +1 more source
Mitotic cell death induction by targeting the mitotic spindle with tubulin-inhibitory indole derivative molecules [PDF]
, 2017 Tubulin-targeting molecules are widely used cancer therapeutic agents. They inhibit microtubule-based structures, including the mitotic spindle, ultimately preventing cell division.
Coluccia, Antonio +10 more
core +1 more source
Pediatric Blood &Cancer, EarlyView.ABSTRACT We sought to identify potential early risk biomarkers for lung disease in children post‐allogeneic HCT. Patients with pulmonary function tests 3 months post‐transplant and plasma samples between days 7 and 14 post‐HCT were included. Six of 27 subjects enrolled had reduced forced expiratory volume 1 (FEV1) z scores.
Isabella S. Small +3 more
wiley +1 more source
STAR ProtocolsSummary: The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures.
Stina Oredsson +3 more
doaj +1 more source
Visualizing Cellular Dynamics and Protein Localization in 3D Collagen
STAR Protocols, 2020 Summary: Immune cells migrate and communicate through cell-to-cell interactions and cytokines to coordinate the specificity and timing of the immune response.
Wan Hon Koh +3 more
doaj +1 more source
STAR Protocols, 2021 Summary: A key facet of epithelial differentiation is the assembly of actin-based protrusions known as microvilli, which amplify apical membrane surface area for various cell functions. To probe mechanisms of microvillus assembly, we developed a protocol
Isabella M. Gaeta +2 more
doaj +1 more source
Practical Characterization of Cell-Electrode Electrical Models in Bio-Impedance Assays [PDF]
, 2018 This paper presents the fitting process followed to adjust the parameters of the electrical model associated to a cell-electrode system in Electrical Cell-substrate Impedance Spectroscopy (ECIS) technique, to the experimental results from cell-culture ...
Daza Navarro, María Paula +7 more
core +1 more source