Results 51 to 60 of about 4,481,323 (329)

The immunological interface: dendritic cells as key regulators in metabolic dysfunction‐associated steatotic liver disease

FEBS Letters, EarlyView.
Metabolic dysfunction‐associated steatotic liver disease (MASLD) affects nearly one‐third of the global population and poses a significant risk of progression to cirrhosis or liver cancer. Here, we discuss the roles of hepatic dendritic cell subtypes in MASLD, highlighting their distinct contributions to disease initiation and progression, and their ...
Camilla Klaimi, WanTing Kong, Camille Blériot, Joel T. Haas   +3 more
wiley   +1 more source

Measurement of autophagy via LC3 western blotting following DNA-damage-induced senescence

STAR Protocols, 2022
Summary: Senescent cells accumulation is associated with aging and age-related diseases, and recent findings suggest that autophagy, the activity of the intracellular degradation system, decreases during senescence.
Hitomi Yamamoto-Imoto, Eiji Hara, Shuhei Nakamura, Tamotsu Yoshimori   +3 more
doaj  

Insights into PI3K/AKT signaling in B cell development and chronic lymphocytic leukemia

FEBS Letters, EarlyView.
This Review explores how the phosphoinositide 3‐kinase and protein kinase B pathway shapes B cell development and drives chronic lymphocytic leukemia, a common blood cancer. It examines how signaling levels affect disease progression, addresses treatment challenges, and introduces novel experimental strategies to improve therapies and patient outcomes.
Maike Buchner
wiley   +1 more source

A quantitative approach for measuring the reservoir of latent HIV-1 proviruses. [PDF]

, 2019
A stable latent reservoir for HIV-1 in resting CD4+ T cells is the principal barrier to a cure1-3. Curative strategies that target the reservoir are being tested4,5 and require accurate, scalable reservoir assays.
Antar, Annukka AR, Beg, Subul A, Bender, Alexandra M, Bertagnolli, Lynn N, Blankson, Joel N, Bruner, Katherine M, Bushman, Frederic D, Capoferri, Adam A, Deeks, Steven G, Fray, Emily J, Gregg, John, Ho, Ya-Chi, Jenike, Katharine M, Kufera, Joshua T, Kwon, Kyungyoon J, Laird, Gregory M, Margolick, Joseph B, Nobles, Christopher, Sengupta, Srona, Siliciano, Janet D, Siliciano, Robert F, Simonetti, Francesco R, Timmons, Andrew, Wada, Nikolas, Wang, Zheng, Zhang, Hao   +25 more
core   +1 more source

A–C Estrogens as Potent and Selective Estrogen Receptor-Beta Agonists (SERBAs) to Enhance Memory Consolidation under Low-Estrogen Conditions [PDF]

, 2018
Estrogen receptor-beta (ERβ) is a drug target for memory consolidation in postmenopausal women. Herein is reported a series of potent and selective ERβ agonists (SERBAs) with in vivo efficacy that are A–C estrogens, lacking the B and D estrogen rings ...
Donaldson, William A., Frick, Karyn M., Hanson, Alicia M., Imhoff, Andrea, Kim, Jaekyoon, Lu, Xingyun, Mackinnon, Alexander Craig, Pandey, Rajesh K., Perera, K. L. Iresha Sampathi, Sem, Daniel S., Sweeney, Noreena, Van Hart, Rochelle M., Wargolet, Adam J.   +12 more
core   +4 more sources

Taurine promotes glucagon‐like peptide‐1 secretion in enteroendocrine L cells

FEBS Letters, EarlyView.
Taurine, a sulfur‐containing amino acid, is likely taken up by enteroendocrine L cells via the taurine transporter. This process increases the levels of cytosolic ATP. The increase in intracellular Ca2+ concentrations and glucagon‐like peptide‐1 secretion through membrane depolarization is caused by the closure of ATP‐sensitive potassium channels ...
Yuri Osuga, Kazuki Harada, Takuya Yamauchi, Tetsuya Kitaguchi, Masami Yokota Hirai, Mitsuharu Matsumoto, Takashi Tsuboi   +6 more
wiley   +1 more source

Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry

STAR Protocols, 2023
Summary: Here, we present a protocol for assessing metabolic activity of bacterial populations by measuring heat flow using isothermal calorimetry. We outline the steps for preparing the different growth models of Pseudomonas aeruginosa and performing ...
Katrin Beilharz, Kasper Nørskov Kragh, Blaine Fritz, Julius B. Kirkegaard, Tim Tolker-Nielsen, Thomas Bjarnsholt, Mads Lichtenberg   +6 more
doaj  

Implementation and Challenges of Direct Acoustic Dosing into Cell-Based Assays

Journal of laboratory automation, 2016
Since the adoption of Labcyte Echo Acoustic Droplet Ejection (ADE) technology by AstraZeneca in 2005, ADE has become the preferred method for compound dosing into both biochemical and cell-based assays across AstraZeneca research and development globally.
K. Roberts, R. Callis, Tim Ikeda, A. Paunovic, C. Simpson, E. Tang, N. Turton, G. Walker   +7 more
semanticscholar   +1 more source

Iterative in Situ Click Chemistry Assembles a Branched Capture Agent and Allosteric Inhibitor for Akt1 [PDF]

, 2011
We describe the use of iterative in situ click chemistry to design an Akt-specific branched peptide triligand that is a drop-in replacement for monoclonal antibodies in multiple biochemical assays.
Agnew, Heather D., Deyle, Kaycie M., Heath, James R., Hein, Jason, Henning, Ryan K., Kwong, Gabriel A., Lee, Su Seong, Lim, Jaehong, Millward, Steven W., Nag, Arundhati, Pfeilsticker, Jessica A., Pitram, Suresh, Sharpless, K. Barry   +12 more
core   +2 more sources

Making tau amyloid models in vitro: a crucial and underestimated challenge

FEBS Letters, EarlyView.
This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley   +1 more source