CO2-elevated cell-free protein synthesis [PDF]
Synthetic and Systems Biotechnology, 2022 Gases are the vital nutrition of all organisms as the precursor of metabolism pathways. As a potential biological process, protein synthesis is inevitably regulated by gas transport and utilization.
Xiaomei Lin +7 more
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Physical stimuli-responsive cell-free protein synthesis [PDF]
Synthetic and Systems Biotechnology, 2020 Cell-free protein synthesis has been developed as a critical platform in synthetic biology. Unlike the cell-based synthesis system, cell-free system activates transcriptional and translational mechanisms in vitro, and can control protein synthesis by ...
Junzhu Yang, Yuan Lu
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Advancing synthetic biology through cell-free protein synthesis [PDF]
Computational and Structural Biotechnology Journal, 2023 The rapid development of synthetic biology has enabled the production of compounds with revolutionary improvements in biotechnology. DNA manipulation tools have expedited the engineering of cellular systems for this purpose.
Ke Yue, Junyu Chen, Yingqiu Li, Lei Kai
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IRES-mediated Pichia pastoris cell-free protein synthesis [PDF]
Bioresources and Bioprocessing, 2023 Cell-free protein synthesis (CFPS) system is an ideal platform for fast and convenient protein research and has been used for macromolecular assembly, unnatural amino acid embedding, glycoprotein production, and more.
Yanan Wang +3 more
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“Cell-Free Synthetic Biology”: Synthetic Biology Meets Cell-Free Protein Synthesis [PDF]
Methods and Protocols, 2019 Since Nirenberg and Matthaei used cell-free protein synthesis (CFPS) to elucidate the genetic code in the early 1960s [...]
Seok Hoon Hong
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Supramolecular protein assembly in cell-free protein synthesis system [PDF]
Bioresources and Bioprocessing, 2022 Protein-based biomaterials have the characteristics of stability and biocompatibility. Based on these advantages, various bionic materials have been manufactured and used in different fields.
Zhixia Li +6 more
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Multiple Gene Expression in Cell-Free Protein Synthesis Systems for Reconstructing Bacteriophages and Metabolic Pathways [PDF]
Microorganisms, 2022 As a fast and reliable technology with applications in diverse biological studies, cell-free protein synthesis has become popular in recent decades. The cell-free protein synthesis system can be considered a complex chemical reaction system that is also ...
Anwesha Purkayastha +2 more
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Cell-Free Protein Synthesis by Diversifying Bacterial Transcription Machinery [PDF]
BioTech, 2021 We have evaluated several approaches to increase protein synthesis in a cell-free coupled bacterial transcription and translation system. A strong pargC promoter, originally isolated from a moderate thermophilic bacterium Geobacillus stearothermophilus ...
Marina Snapyan +5 more
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Modeling Cell-Free Protein Synthesis Systems—Approaches and Applications [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 In vitro systems are ideal setups to investigate the basic principles of biochemical reactions and subsequently the bricks of life. Cell-free protein synthesis (CFPS) systems mimic the transcription and translation processes of whole cells in a ...
Jan Müller +2 more
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Production of Recombinant Horseradish Peroxidase in an Engineered Cell-free Protein Synthesis System [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 One of the main advantages of a cell-free synthesis system is that the synthetic machinery of cells can be modularized and re-assembled for desired purposes.
Yu-Jin Park, Dong-Myung Kim
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