Improved cell-free RNA and protein synthesis system. [PDF]
PLoS ONE, 2014 Cell-free RNA and protein synthesis (CFPS) is becoming increasingly used for protein production as yields increase and costs decrease. Advances in reconstituted CFPS systems such as the Protein synthesis Using Recombinant Elements (PURE) system offer new
Jun Li +3 more
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Cancer Medicine, 2023 Purpose The pathogenesis of cancers depends on the molecular background of each individual patient. Therefore, verifying as many biomarkers as possible and clarifying their relationships with each disease status would be very valuable.
Keiko Kasahara +9 more
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Site-Specific Cleavage of Ribosomal RNA in Escherichia coli-Based Cell-Free Protein Synthesis Systems. [PDF]
PLoS ONE, 2016 Cell-free protein synthesis, which mimics the biological protein production system, allows rapid expression of proteins without the need to maintain a viable cell.
Jurek Failmezger +4 more
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Cell-free protein synthesis for proteomics [PDF]
Briefings in Functional Genomics and Proteomics, 2004 The use of cell-free expression systems as an alternative to cell-based methods for protein production is greatly facilitating studies of protein functions. Recent improvements to cell-free systems, and the development of cell-free protein display and microarray technologies, have led to cell-free protein synthesis becoming a powerful tool for large ...
Alison M, Jackson +3 more
openaire +2 more sources
A continuous-exchange cell-free protein synthesis system based on extracts from cultured insect cells. [PDF]
PLoS ONE, 2014 In this study, we present a novel technique for the synthesis of complex prokaryotic and eukaryotic proteins by using a continuous-exchange cell-free (CECF) protein synthesis system based on extracts from cultured insect cells.
Marlitt Stech +5 more
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Glycosylation-on-a-Chip: A Flow-Based Microfluidic System for Cell-Free Glycoprotein Biosynthesis
Frontiers in Molecular Biosciences, 2021 In recent years, cell-free synthetic glycobiology technologies have emerged that enable production and remodeling of glycoproteins outside the confines of the cell.
Alicia K. Aquino +4 more
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Optimising protein synthesis in cell‐free systems, a review
Engineering Biology, 2021 Over the last decades, cell‐free systems have been extensively used for in vitro protein expression. A vast range of protocols and cellular sources varying from prokaryotes and eukaryotes are now available for cell‐free technology.
Angelo C. Batista +3 more
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Biochemical aspects of bacterial strategies for handling the incomplete translation processes
Frontiers in Microbiology, 2014 During protein synthesis in cells, translating ribosomes may encounter abnormal situations that lead to retention of immature peptidyl-tRNA on the ribosome due to failure of suitable termination processes.
Yoshihiro eShimizu
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Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system. [PDF]
, 2018 Protein degradation is a fundamental process in all living cells and is essential to remove both damaged proteins and intact proteins that are no longer needed by the cell.
Joseph, Simpson +4 more
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The Regulatory Role of MeAIB in Protein Metabolism and the mTOR Signaling Pathway in Porcine Enterocytes. [PDF]
, 2018 Amino acid transporters play an important role in cell growth and metabolism. MeAIB, a transporter-selective substrate, often represses the adaptive regulation of sodium-coupled neutral amino acid transporter 2 (SNAT2), which may act as a receptor and ...
Ji, Peng +5 more
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