Transport of Cytoplasmically Synthesized Proteins into the Mitochondria in a Cell Free System from Neurospora crassa [PDF]
, 1977 Synthesis and transport of mitochondrial proteins were followed in a cell-free homogenate of Neurospora crassa in which mitochondrial translation was inhibited. Proteins synthesized on cytoplasmic ribosomes are transferred into the mitochondrial fraction.
Andrews T. M. +30 more
core +1 more source
Entropy, 2022 The underlying mechanism determining the size of a particular cell is one of the fundamental unknowns in cell biology. Here, using a new approach that could be used for most of unicellular species, we show that the protein synthesis and cell size are ...
Mohammad Razavi +2 more
doaj +1 more source
Artificial environments for the co-translational stabilization of cell-free expressed proteins [PDF]
, 2013 An approach for designing individual expression environments that reduce or prevent protein aggregation and precipitation is described. Inefficient folding of difficult proteins in unfavorable translation environments can cause significant losses of ...
Bernhard, Frank +3 more
core +2 more sources
The 5 ' untranslated region of protein kinase C delta directs translation by an internal ribosome entry segment that is most active in densely growing cells and during apoptosis. [PDF]
, 2002 Protein kinase Cdelta (PKCdelta) is a member of the PKC family of phospholipid-dependent serine/threonine kinases and is involved in cell proliferation, apoptosis, and differentiation.
Morrish, B.C., Rumsby, M.G.
core +2 more sources
Bacterial cell-free expression technology to in vitro systems engineering and optimization
Synthetic and Systems Biotechnology, 2017 Cell-free expression system is a technology for the synthesis of proteins in vitro. The system is a platform for several bioengineering projects, e.g.
Filippo Caschera
doaj +1 more source
The histone deacetylase inhibiting drug Entinostat induces lipid accumulation in differentiated HepaRG cells [PDF]
, 2016 Dietary overload of toxic, free metabolic intermediates leads to disrupted insulin signalling and fatty liver disease. However, it was recently reported that this pathway might not be universal: depletion of histone deacetylase (HDAC) enhances insulin ...
Enejder, Annika +6 more
core +1 more source
Bioengineering, 2023 The XpressCF+® cell-free protein synthesis system is a robust platform for the production of non-natural amino acids containing antibodies, which enable the site-specific conjugation of homogeneous antibody drug conjugates (ADCs) via click chemistry ...
Jeffrey Hanson +15 more
doaj +1 more source
Cell‐free protein synthesis [PDF]
The FEBS Journal, 2006 Cell-free protein synthesis using cell extracts from Escherichia coli, wheat germ and rabbit reticulocytes has been used for over 40 years to produce small amounts of radiolabeled proteins for identification of gene products and other applications. In the E.
openaire +2 more sources
Cell-Free Protein Synthesis v1 [PDF]
, 2020 This is part 3.3 of the "A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties" collection of protocols: https://www.protocols.io/view/a-combined-cell-free-protein-synthesis-and-fluores-bqntmven Collection Abstract: Fluorescent labeling of de novo synthesized proteins is in particular a valuable ...
Anne Zemella +3 more
openaire +1 more source
Inhibition of RNA polymerase II transcription in human cells by synthetic DNA-binding ligands [PDF]
, 1998 Sequence-specific DNA-binding small molecules that can permeate human cells potentially could regulate transcription of specific genes. Multiple cellular DNA-binding transcription factors are required by HIV type 1 for RNA synthesis.
Baird, Eldon E. +6 more
core +1 more source