Results 211 to 220 of about 1,066,214 (229)

Messenger RNA turnover in cell-free extracts

1990
Publisher Summary There are several advantages to using an in vitro system for studying mRNA turnover. The polysome is the most important component of the cell-free mRNA decay system we have described. It contains both the mRNA to be degraded and the enzymes and cofactors that degrade the mRNA. The major criterion used to assess the reliability and
G, Brewer, J, Ross
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Cell-Free RNA Sequencing from Biofluid Samples

2023
Liquid biopsy has obvious advantages over invasive sampling methods. Cell-free DNA is now widely used in prenatal and cancer diagnosis. Cell-free RNA is relatively unstable but could contain potential biomarkers, and some studies have demonstrated that cfRNA sequencing may be developed to be a promising diagnostic assay for disease detection.
Lili, Zhang, Xingyu, Liu, Chen, Wang, Xiaoshuang, Li, Geng, Tian, Weiwei, Wang   +5 more
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Cell-free translation of influenza virus messenger RNA

Virology, 1973
Abstract The addition of total RNA from influenza virus-infected chick embryo cells to a cell-free rabbit reticulocyte protein-synthesizing system programmed the synthesis of a polypeptide with the electrophoretic mobility and antigenicity of influenza virus matrix (M) polypeptide.
D W, Kingsbury, R G, Webster
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Cell-free translation of avian erythroblastosis virus RNA

Journal of Virology, 1980
Avian erythroblastosis virus (AEV) RNA rescued from nonproducer cells by superinfection with a helper virus is translated into three polypeptides in the messenger-dependent rabbit reticulocyte lysate. A 75,000 molecular weight polypeptide (P75AEV) is synthesized from 28S RNA and is encoded by the 5' section of the AEV RNA, including gag-related and AEV-
T, Pawson, G S, Martin
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Cell‐free RNA for the liquid biopsy of gastrointestinal cancer

WIREs RNA, 2023
AbstractGastrointestinal (GI) cancer includes many cancer types, such as esophageal, liver, gastric, pancreatic, and colorectal cancer. As the cornerstone of personalized medicine for GI cancer, liquid biopsy based on noninvasive biomarkers provides promising opportunities for early diagnosis and dynamic treatment management. Recently, a growing number
Shaozhen Xing, Yumin Zhu, Yaxian You, Siqi Wang, Hongke Wang, Meng Ning, Heyue Jin, Zhengxia Liu, Xinhua Zhang, Chunzhao Yu, Zhi John Lu   +10 more
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Isolation and cell-free translation of immunoglobulin messenger RNA

Archives of Biochemistry and Biophysics, 1976
Abstract The mRNA's for both the heavy chain (H 315 ) and the light chain (L 315 ) of the mineral oil-induced plasmacytoma-315 myeloma protein have been isolated and partially purified from both total cellular RNA and RNA derived from membrane-bound polysomes. The yields of both L 315 mRNA and, in particular, of H 315 mRNA were increased when total
M, Green, T, Zehavi-Willner, P N, Graves, J, McInnes, S, Pestka   +4 more
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The potential of circulating cell free RNA as a biomarker in cancer

Expert Review of Molecular Diagnostics, 2019
Introduction: It is now clear that circulating cell-free ribonucleic acids (ccfRNAs), including messenger RNA (mRNA) and miRNA, are potential cancer biomarkers. As ccfmiRNA is relatively more stable than ccfmRNA, research should concentrate on developing novel methods to preserve the stability of ccfmRNA and standardization of the protocol which ...
Ka Wan Emily Cheung, Sin-yu Rachel Choi, Lok Ting Claire Lee, Nga Lam Ella Lee, Hin Fung Tsang, Yin Tung Cheng, William Chi Shing Cho, Elaine Yue Ling Wong, Sze Chuen Cesar Wong   +8 more
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Cell-free protein synthesis dependent on transfer RNA

Biochemical and Biophysical Research Communications, 1966
Abstract Endogenous transfer RNA was removed by gel filtration from 30,000 × g extracts of Escherichia , coli . The preparations were active for cell-free protein synthesis after the addition of both t-RNA and message RNA. The system provided a method to study various t-RNA preparations in the translation of specific messages.
P P, Hung, L, Straube, L R, Overby
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Synthesis of Tryptophan Operon RNA in a Cell-free System

Nature New Biology, 1973
DNA of the tryptophan operon is transcribed in cytoplasmic extracts of E. coli. This transcription and its regulation by repression, occur in the absence of ribosomes. Repression requires the repressor protein product of the trp R gene and tryptophan; no involvement of the tryptophanyl-tRNA synthetase is found.
D, McGeoch, J, McGeoch, D, Morse
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Isolation of Cell-Free RNA from Maternal Plasma

2008
The discovery of cell-free RNA in plasma and serum samples provides possibilities for noninvasive prenatal diagnosis. Quantitative alterations of cell-free placental-derived mRNA in the maternal circulation are associated with many pregnancy-related disorders, such as preeclampsia and preterm labor.
Zhong, Xiao Yan, Holzgreve, Wolfgang, Huang, Dorothy J.   +2 more
openaire   +3 more sources