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Cell-free translation of messenger RNA for human bisphospho-glyceromutase
Biochemical and Biophysical Research Communications, 1984mRNAs prepared from different human tissues were translated in a cell-free reticulocyte lysate system and, when present, the neosynthesized bisphosphoglyceromutase ( BPGM ) was specifically isolated by immunoprecipitation and analyzed by polyacrylamide gel electrophoresis.
A, Dubart +5 more
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Translation of Globin Messenger RNA in a Heterologous Cell-free System
Nature New Biology, 1971MESSENGER-SPECIFIC initiation factors, capable of discriminating between classes of messenger RNAs (mRNAs) or different cistrons in viral RNA, have been implicated in the regulation of protein synthesis in bacteria1–5. Comparable but less detailed observations have also been made in eukaryotic systems6–10.
M B, Mathews, M, Osbron, J B, Lingrel
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The formation of immunogenic RNA-antigen complexes in a cell-free system
Cellular Immunology, 1973Abstract The formation of complexes between ribonucleic acid (RNA) and solubilized bacteriophage T2 in vitro is readily demonstrable. The ability to evoke antibody formation against T2 in lymph node cultures is not a property of all such complexes but rather is restricted to those formed in the presence of cell sap (100,000 g supernatant). Further
M, Fishman, F L, Adler
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Translation of TMV-RNA in a cell-free wheat embryo system
Virology, 1973Abstract The products obtained from the incubation of TMV-RNA in a cell-free amino acid incorporation system from wheat embryos were analyzed by SDS gel electrophoresis and Sephadex G-200 chromatography. A minor fraction of the products coincided with authentic viral coat protein on gel electrophoresis. Comparison of a tryptic digest of this fraction
D, Efron, A, Marcus
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Poliovirus RNA recombination in cell-free extracts.
RNA (New York, N.Y.), 1997Poliovirus RNA has been shown to undergo homologous genetic recombination at a high frequency in infected human cells. Recently it has become possible to mimic the entire intracellular replicative cycle of poliovirus replication in cytoplasmic extracts prepared from HeLa cells, resulting in the generation of infectious poliovirions.
R S, Tang +3 more
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Cell-free translation of cartilage RNAs.
The Alabama journal of medical sciences, 1981W B, Upholt, B M, Vertel, A, Dorfman
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DNA Aids in Cell-Free Synthesis of RNA
Chemical & Engineering News Archive, 1961openaire +1 more source
Techniques for the cell-free translation of viral RNA.
Laboratory and research methods in biology and medicine, 1982D E, Hruby, L A, Ball
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