Results 51 to 60 of about 9,966,852 (260)

Gene circuit-based sensors

Fundamental Research
As an important branch of biosensors, gene circuit-based biosensors constructed based on synthetic biology concepts can sense specific substances to be measured and converted into specific signal outputs.
Xinyue Guo, Min Li, Xiaolei Zuo
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane. [PDF]

FEBS Open Bio
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Ghosh S, Mukherjee K, Bhattacharyya SN.
europepmc   +2 more sources

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

A Method for Fast, High-Precision Characterization of Synthetic Biology Devices [PDF]

, 2012
Engineering biological systems with predictable behavior is a foundational goal of synthetic biology. To accomplish this, it is important to accurately characterize the behavior of biological devices.
Weiss, Ron, Adler, Aaron, Beal, Jacob, Yaman, Fusun, Davidsohn, Noah   +4 more
core  

Context-aware synthetic biology by controller design: Engineering the mammalian cell

, 2021
The rise of systems biology has ushered a new paradigm: the view of the cell as a system that processes environmental inputs to drive phenotypic outputs.
Shakiba, Nika, Jones, Ross D, Nika Shakiba, Ross D. Jones, Weiss, Ron, Jones, Ross D., Domitilla Del Vecchio, Del Vecchio, Domitilla, Ron Weiss   +8 more
core   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Evolutionary cell biology: Functional insight from “Endless forms most beautiful” [PDF]

, 2015
In animal and fungal model organisms, the complexities of cell biology have been analyzed in exquisite detail and much is known about how these organisms function at the cellular level.
Richardson, Elizabeth, Dacks, Joel B., Tsaousis, Anastasios D., Dorrell, Richard G., Zerr, Kelly   +4 more
core   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Varieties of Noise: Analogical Reasoning in Synthetic Biology [PDF]

, 2013
The picture of synthetic biology as a kind of engineering science has largely created the public understanding of this novel field, covering both its promises and risks.
Knuuttila, Tarja, Loettgers, Andrea
core