A curcumin direct protein biosensor for cell‐free prototyping
Engineering Biology, 2022 In synthetic biology, biosensors are routinely coupled with a gene expression system for detecting small molecules and physical signals. We reveal a fluorescent complex, based on the interaction of an Escherichia coli double bond reductase (EcCurA), as a
Agata Kennedy +4 more
doaj +1 more source
A Conserved Cell Growth Cycle Can Account for the Environmental Stress Responses of Divergent Eukaryotes [PDF]
, 2012 The respiratory metabolic cycle in budding yeast (Saccharomyces cerevisiae) consists of two phases most simply defined phenomenologically: low oxygen consumption (LOC) and high oxygen consumption (HOC).
Airoldi, Edoardo| +15 more
core +1 more source
Cell-Free Characterization of Coherent Feed-Forward Loop-Based Synthetic Genetic Circuits [PDF]
, 2021 Regulatory pathways inside living cells employ feed-forward architectures to fulfill essential signal processing functions that aid in the interpretation of various types of inputs through noise-filtering, fold-change detection and adaptation.
van der Linden, Ardjan J. +26 more
core +3 more sources
Applications of synthetic biology in medical and pharmaceutical fields
Signal Transduction and Targeted Therapy, 2023 Synthetic biology aims to design or assemble existing bioparts or bio-components for useful bioproperties. During the past decades, progresses have been made to build delicate biocircuits, standardized biological building blocks and to develop various ...
Xu Yan +3 more
doaj +1 more source
Methodologies for preparation of prokaryotic extracts for cell-free expression systems
Synthetic and Systems Biotechnology, 2020 Cell-free systems that mimic essential cell functions, such as gene expression, have dramatically expanded in recent years, both in terms of applications and widespread adoption.
Stephanie D. Cole +4 more
doaj +1 more source
Build-a-Cell: Engineering a Synthetic Cell Community
Life, 2021 Build-a-Cell is a global network of researchers that aims to develop synthetic living cells within the next decade. These cells will revolutionize the biotechnology industry by providing scientists and engineers with a more complete understanding of ...
Caroline Frischmon +3 more
doaj +1 more source
Synthetic and Systems Biotechnology, 2018 Advances in metabolic engineering and synthetic biology have facilitated the manufacturing of many valuable-added compounds and commodity chemicals using microbial cell factories in the past decade.
Lihong Jiang +3 more
doaj +1 more source
UME6, a novel filament-specific regulator of Candida albicans hyphal extension and virulence [PDF]
, 2008 Publications prior to 2012 under name Thompson, D.S.Peer ...
Monteagudo, Carlos +7 more
core +1 more source
The Usual Suspects 2019: of Chips, Droplets, Synthesis, and Artificial Cells
Micromachines, 2019 Synthetic biology aims to understand fundamental biological processes in more detail than possible for actual living cells. Synthetic biology can combat decomposition and build-up of artificial experimental models under precisely controlled and defined ...
Christoph Eilenberger +5 more
doaj +1 more source
Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles
Nature Communications, 2021 Cell-free gene expression systems are an attractive platform for biomanufacturing and synthetic biology. Here the authors characterize native membrane vesicles in E. coli extracts for improved glycoengineering.
Jasmine M. Hershewe +8 more
doaj +1 more source