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Development of prokaryotic cell-free systems for synthetic biology [PDF]
, 2016 ABSTRACT Prokaryotic cell-free systems are currently heavily used for the production of protein that can be otherwise challenging to produce in cells. However, historically cell-free systems were used to explore natural phenomena before the advent of genetic modification and transformation technology.
Chiao, Abel C. +2 more
openaire +3 more sources
Cell-free synthetic biology for in vitro prototype engineering. [PDF]
Biochem Soc Trans, 2017 Cell-free transcription–translation is an expanding field in synthetic biology as a rapid prototyping platform for blueprinting the design of synthetic biological devices. Exemplar efforts include translation of prototype designs into medical test kits for on-site identification of viruses (Zika and Ebola), while gene circuit cascades can be tested ...
Moore SJ, MacDonald JT, Freemont PS.
europepmc +5 more sources
Cell-Free Synthetic Biology: Engineering Beyond the Cell. [PDF]
Cold Spring Harb Perspect Biol, 2016 Cell-free protein synthesis (CFPS) technologies have enabled inexpensive and rapid recombinant protein expression. Numerous highly active CFPS platforms are now available and have recently been used for synthetic biology applications. In this review, we focus on the ability of CFPS to expand our understanding of biological systems and its applications ...
Perez JG, Stark JC, Jewett MC.
europepmc +4 more sources
Microorganisms, 2022 As a fast and reliable technology with applications in diverse biological studies, cell-free protein synthesis has become popular in recent decades. The cell-free protein synthesis system can be considered a complex chemical reaction system that is also ...
Anwesha Purkayastha +2 more
doaj +1 more source
Cell-free biology: exploiting the interface between synthetic biology and synthetic chemistry [PDF]
Current Opinion in Biotechnology, 2012 Just as synthetic organic chemistry once revolutionized the ability of chemists to build molecules (including those that did not exist in nature) following a basic set of design rules, cell-free synthetic biology is beginning to provide an improved toolbox and faster process for not only harnessing but also expanding the chemistry of life.
D Calvin, Harris, Michael C, Jewett
openaire +2 more sources
Cell-free protein synthesis as a prototyping platform for mammalian synthetic biology [PDF]
, 2020 The field of mammalian synthetic biology is expanding quickly, and technologies for engineering large synthetic gene circuits are increasingly accessible. However, for mammalian cell engineering, traditional tissue culture methods are slow and cumbersome,
McClymont, David W +9 more
core +1 more source
Synthetic biology—putting engineering into biology [PDF]
, 2006 Synthetic biology is interpreted as the engineering-driven building of increasingly complex biological entities for novel applications. Encouraged by progress in the design of artificial gene networks, de novo DNA synthesis and protein engineering, we ...
Panke, Sven +3 more
core +1 more source
Engineering control circuits for molecular robots using synthetic biology
APL Materials, 2020 The integration of molecular robots and synthetic biology allows for the creation of sophisticated behaviors at the molecular level. Similar to the synergy between bioelectronics and soft robotics, synthetic biology provides control circuitry for ...
Ting-Yen Wei, Warren C. Ruder
doaj +1 more source
Cell-free synthetic biology for natural product biosynthesis and discovery.
Chem Soc RevCell free synthetic biology enables a broad range of approaches relevant to the generation, biosynthetic characterisation, bioengineering, activity profiling, and production of complex natural products.
Rice AJ +6 more
europepmc +4 more sources
Synthetic biology of fungal natural products [PDF]
, 2015 This work was supported by the DFG-funded excellence graduate school Jena School for Microbial Communication (JSMC; DJM, AAB), the collaborative research center (SFB) 1127 Chemical Mediators in Complex Biosystems (ChemBioSys—AAB, project B02) and the ...
Vito Valiante +7 more
core +1 more source