Results 91 to 100 of about 6,713,855 (257)

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8+ T cells

STAR Protocols
Summary: Here, we present a protocol for the quantitative characterization of human T cell aging. We describe steps for sample collection; peripheral blood mononuclear cell (PBMC) isolation; and the enrichment, assessment, and activation of naive CD8+ T ...
Linlin Li, Jun Jin
doaj   +1 more source

DIE-RNA: A Reproducible Strategy for the Digestion of Normal and Injured Pancreas, Isolation of Pancreatic Cells from Genetically Engineered Mouse Models and Extraction of High Quality RNA

Frontiers in Physiology, 2018
The isolation of ribonucleic acid (RNA) suitable for gene expression studies is challenging in the pancreas, due to its high ribonuclease activity. This is even more complicated during pancreatitis, a condition associated with inflammation and fibrosis ...
Mohamad Assi, Nicolas Dauguet, Patrick Jacquemin   +2 more
doaj   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress

STAR Protocols
Summary: Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models.
Hyun Young Kim, Wonseok Lee, Xiao Liu, Haeum Jang, Sadatsugu Sakane, Raquel Carvalho-Gontijo Weber, Karin Diggle, Samuel A. Kerk, Christian M. Metallo, Tatiana Kisseleva, David A. Brenner   +10 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

An efficient isolation method for pericytes of the cochlear stria vascularis

Shanghai Jiaotong Daxue xuebao. Yixue ban
Objective·To develop a stable and efficient method for isolating and obtaining pericytes from the cochlear stria vascularis while preserving their original physicochemical properties as much as possible.Methods·Stria vascularis tissues were dissected ...
ZHU Yue, LI Xiaoqin, WANG Wenxiao, ZHOU Lingyun, WU Hao, TAO Yong, DU Tingting   +6 more
doaj   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Cell lineage specific distribution of H3K27 trimethylation accumulation in an in vitro model for human implantation [PDF]

, 2012
Female mammals inactivate one of their two X-chromosomes to compensate for the difference in gene-dosage with males that have just one X-chromosome. X-chromosome inactivation is initiated by the expression of the non-coding RNA Xist, which coats the X ...
Weimar, Charlotte H. E., Weimar, C.H., Heijnen Cobi J., Kuijk Ewart W., Kuijk, E.W., Fauser, B.C., Lopes, S.M.C.D., Bart C J M Fauser, Ewart W Kuijk, Geijsen Niels, Weimar Charlotte H. E., Macklon Nick, Kuijk, Ewart W., Teklenburg, G., Fauser Bart C. J. M., Susana M Chuva de Sousa Lopes, Macklon, N., Teklenburg Gijs, Macklon, Nick, Niels Geijsen, Lopes, S.M., Weimar, C.H.E., Fauser, Bart C. J. M., Teklenburg, Gijs, Geijsen, Niels, Nick Macklon, Geijsen, N., Cobi J Heijnen, Charlotte H E Weimar, Heijnen, Cobi J., Fauser, B.C.J.M., Heijnen, C.J., Chuva de Sousa Lopes Susana M., Chuva de Sousa Lopez, Susana M., Gijs Teklenburg   +34 more
core   +1 more source

Timing the Landmark Events in the Evolution of Clear Cell Renal Cell Cancer: TRACERx Renal.

, 2022
Clear cell renal cell carcinoma (ccRCC) is characterized by near-universal loss of the short arm of chromosome 3, deleting several tumor suppressor genes. We analyzed whole genomes from 95 biopsies across 33 patients with clear cell renal cell carcinoma.
Raine, K, Yates, LR, Teague, J, Lopez, JI, Gore, M, Rowan, A, Mitchell, TJ, Lynch, AG, Au, L, Stewart, A, Litchfield, K, Turajlic, S, Tarpey, P, Chandra, A, Csabai, I, Swanton, C, Fotiadis, N, Lynch, J, Soultati, A, Challacombe, B, Xu, H, Hazell, S, Stamp, G, Elaidi, R, Horswell, S, Campbell, PJ, Chowdhury, S, Butler, AP, Rudman, S, Wedge, DC, Futreal, PA, Nicol, D, Farmery, JHR, O'Brien, T, Maura, F, McGranahan, N, TRACERx Renal Consortium,, Spain, L, Stewart, GD, Szallasi, Z, Martincorena, I, Stares, M, Fernando, A, Larkin, J, Oudard, S, Pickering, L, Chambers, T, Angelopoulos, N   +47 more
core   +1 more source