Results 51 to 60 of about 319,319 (301)

Applications of cell sorting in biotechnology [PDF]

open access: yesMicrobial Cell Factories, 2006
Abstract Due to its unique capability to analyze a large number of single cells for several parameters simultaneously, flow cytometry has changed our understanding of the behavior of cells in culture and of the population dynamics even of clonal populations.
Mattanovich Diethard, Borth Nicole
openaire   +3 more sources

Sorting live stem cells based on Sox2 mRNA expression. [PDF]

open access: yes, 2012
PMCID: PMC3507951This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited ...
Hans M. Larsson (118208)   +44 more
core   +1 more source

Computational modeling reveals that a combination of chemotaxis and differential adhesion leads to robust cell sorting during tissue patterning. [PDF]

open access: yesPLoS ONE, 2014
Robust tissue patterning is crucial to many processes during development. The "French Flag" model of patterning, whereby naïve cells in a gradient of diffusible morphogen signal adopt different fates due to exposure to different amounts of morphogen ...
Rui Zhen Tan, Keng-Hwee Chiam
doaj   +1 more source

An ultrasensitive sorting mechanism for EGF receptor endocytosis [PDF]

open access: yes, 2008
Background The EGF receptor has been shown to internalize via clathrin-independent endocytosis (CIE) in a ligand concentration dependent manner. From a modeling point of view, this resembles an ultrasensitive response, which is the ability of signaling ...
Roland Eils   +9 more
core   +1 more source

Intuitive, Image-Based Cell Sorting Using Optofluidic Cell Sorting

open access: yes, 2016
We present a microfluidic cell-sorting device which augments microscopy with the capability to perform facile image-based cell sorting. This combination enables intuitive, complex phenotype sorting based on spatio−temporal fluorescence or cell morphology.
J. Voldman (2440840)   +1 more
core   +3 more sources

DIE-RNA: A Reproducible Strategy for the Digestion of Normal and Injured Pancreas, Isolation of Pancreatic Cells from Genetically Engineered Mouse Models and Extraction of High Quality RNA

open access: yesFrontiers in Physiology, 2018
The isolation of ribonucleic acid (RNA) suitable for gene expression studies is challenging in the pancreas, due to its high ribonuclease activity. This is even more complicated during pancreatitis, a condition associated with inflammation and fibrosis ...
Mohamad Assi   +2 more
doaj   +1 more source

Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue

open access: yesSTAR Protocols, 2021
Summary: Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types ...
M. Sadman Sakib   +4 more
doaj   +1 more source

Signals involved in protein intracellular sorting [PDF]

open access: yes, 2005
“…Confusion appears to occur just after the articulation of a major conceptual advance that served to greatly clarify a problem of exceptional importance.”- Ira Mellman, 1996.
Nair, Prashant
core   +1 more source

Large-scale simulations of biological cell sorting driven by differential adhesion follow diffusion-limited domain coalescence regime.

open access: yesPLoS Computational Biology, 2021
Cell sorting, whereby a heterogeneous cell mixture segregates and forms distinct homogeneous tissues, is one of the main collective cell behaviors at work during development.
Marc Durand
doaj   +1 more source

Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping and assembly in Solanum species

open access: yesMethodsX, 2018
Next-generation genome mapping through nanochannels (Bionano optical mapping) of plant genomes brings genome assemblies to the ‘nearly-finished’ level for reliable and detailed gene annotations and assessment of structural variations.
Paola Gaiero   +9 more
doaj   +1 more source

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