Results 31 to 40 of about 5,022,243 (208)

Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping and assembly in Solanum species

open access: yesMethodsX, 2018
Next-generation genome mapping through nanochannels (Bionano optical mapping) of plant genomes brings genome assemblies to the ‘nearly-finished’ level for reliable and detailed gene annotations and assessment of structural variations.
Paola Gaiero   +9 more
doaj   +1 more source

Computational modeling reveals that a combination of chemotaxis and differential adhesion leads to robust cell sorting during tissue patterning. [PDF]

open access: yesPLoS ONE, 2014
Robust tissue patterning is crucial to many processes during development. The "French Flag" model of patterning, whereby naïve cells in a gradient of diffusible morphogen signal adopt different fates due to exposure to different amounts of morphogen ...
Rui Zhen Tan, Keng-Hwee Chiam
doaj   +1 more source

Vesicular traffic in polarized epithelial cells : identification and characterization of general and epithelial specific factors [PDF]

open access: yes, 1994
Polarized epithelial cells are differentiated into apical and basolateral plasma membrane domains separated by tight junctions. The apical cell surface usually faces the external milieu, the basolateral domain provides cell-cell and cell-substratum ...
Fiedler, Klaus
core   +1 more source

A Gene Transfer-Positive Cell Sorting System Utilizing Membrane-Anchoring Affinity Tag

open access: yesFrontiers in Bioengineering and Biotechnology, 2022
Gene delivery efficiency is an essential limit factor in gene study and gene therapy, especially for cells that are hard for gene transfer. Here we develop an affinity cell sorting system that allows efficient enrichment of gene transfer-positive cells ...
Lele Yang   +4 more
doaj   +1 more source

The role of motor proteins in endosomal sorting [PDF]

open access: yes, 2011
Microtubule motor proteins play key roles in the spatial organization of intracellular organelles as well as the transfer of material between them.
Hunt, Sylvie   +3 more
core   +1 more source

Evidence‐Informed Multidisciplinary Consensus Guidance for the Psychosocial Care of Adolescents With High‐Risk Cancer: Recommendations From the Italian Association of Pediatric Hematology and Oncology

open access: yesPediatric Blood &Cancer, EarlyView.
ABSTRACT Background Adolescents with high‐risk cancer face complex developmental, psychosocial, and ethical challenges that extend beyond disease‐directed treatment. Although international recommendations exist for communication, psychosocial care, pediatric palliative care, survivorship, and shared decision‐making, these have largely evolved within ...
Johanna M. C. Blom   +15 more
wiley   +1 more source

Transcriptome analysis of classical blood cells reveals downregulation of pro-inflammatory genes in the classical monocytes of long COVID patients

open access: yesFrontiers in Immunology
IntroductionDespite extensive research, the pathogenesis and predispositions underlying long COVID (long-term coronavirus disease 2019) remain poorly understood.MethodsTo address this, we analyzed the immunological landscapes of 44 patients with long ...
Florian Fricke   +10 more
doaj   +1 more source

Raman image-activated cell sorting

open access: yesNature Communications, 2020
Most current cell sorting methods are based on fluorescence detection with no imaging capability. Here the authors generate and use Raman image-activated cell sorting with a throughput of around 100 events per second, providing molecular images with no ...
Nao Nitta   +48 more
doaj   +1 more source

Large-scale simulations of biological cell sorting driven by differential adhesion follow diffusion-limited domain coalescence regime.

open access: yesPLoS Computational Biology, 2021
Cell sorting, whereby a heterogeneous cell mixture segregates and forms distinct homogeneous tissues, is one of the main collective cell behaviors at work during development.
Marc Durand
doaj   +1 more source

Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue

open access: yesSTAR Protocols, 2021
Summary: Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types ...
M. Sadman Sakib   +4 more
doaj   +1 more source

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