Results 181 to 190 of about 15,438,001 (312)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

The measurement of red cell volume [PDF]

The Journal of Physiology, 1933
J, Macleod, E, Ponder
openaire   +2 more sources

The F-Actin of In Situ Chondrocytes in Non-Degenerate and Mildly-Degenerate Human Cartilage: Changes With Cell Volume, and Cytoplasmic Processes. [PDF]

J Orthop Res
Garni MSMA, Amin AK, Hall AC.
europepmc   +1 more source

Investigating the potential of packed cell volume for deducing hemoglobin: Cholistani camels in perspective. [PDF]

PLoS One, 2023
Farooq U, Idris M, Sajjad N, Lashari MH, Ahmad S, Rehman ZU, Rashid H, Mahmood A, Hameed S.   +8 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

A combinatorial transcription factor screening platform for immune cell reprogramming

Direct reprogramming of immune cells holds promise for immunotherapy but is constrained by limited knowledge of transcription factor (TF) networks. Here, we developed REPROcode, a combinatorial single-cell screening platform to identify TF combinations ...
Pereira, Carlos Filipe, Nair, Malavika, Knittel, Ramin, Cajal, Laura Rabanal, Zimmermannová, Olga, Henriques-Oliveira, Luís, Ringnér, Markus, Theis, Fabian J., Martin, Marcel, Klein, Dominik, Sitnicka, Ewa, Pértiga-Cabral, Diogo, Caiado, Inês, Oliveira, Daniel, Cirovic, Branko, Kurochkin, Ilia, Minaeva, Mariia, Feick, Cora, Rosa, Fabio F., Halitzki, Evelyn, Pires, Cristiana F., Altman, Abigail R., Pereira, Carlos Filipe,, Oliveira, Daniel,, Pires, Cristiana F.,, Sitnicka, Ewa,, Kurochkin, Ilia,, Feick, Cora,, Altman, Abigail R.,, Pereira, Carlos-Filipe, Pértiga-Cabral, Diogo,, Henriques-Oliveira, Luís,, Cirovic, Branko,, Caiado, Inês,, Rosa, Fabio F.,, Theis, Fabian J.,, Martin, Marcel,, Nair, Malavika,, Ringnér, Markus,, Halitzki, Evelyn,, Knittel, Ramin,, Klein, Dominik,, Zimmermannová, Olga,, Lund University., Cajal, Laura Rabanal,, Minaeva, Mariia,   +45 more
core   +1 more source

Stability of packed cell volume in dogs with nonregenerative anaemias from immediate to 24 hours posttransfusion. [PDF]

Aust Vet J
Katz ED, Serrano S.
europepmc   +1 more source

Optimal use of red cell volume distribution width-to-platelet ratio to exclude cirrhosis in patients with chronic hepatitis B. [PDF]

Liver Res, 2023
Yu H, Li C, Li M, Liang Z, Smayi A, Yang B, Abassa KK, Chen J, Wu B, Yang Y.   +9 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Piezo1-mediated mechanohydraulic control of cell volume drives cardiac morphogenesis. [PDF]

Sci Adv
Vagena-Pantoula C, Kalyviotis K, Feng S, Sanchez A, Kondrychyn I, Fukumoto M, Pan X, Juan T, Stainier DYR, Pantazis P, Phng LK, Vermot J.   +11 more
europepmc   +1 more source