Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Alexander Leaf, MD (1920 -2012) – A Tribute to a Life in Physiology and Medicine
George E Billman
doaj +1 more source
Estimation of minimum centrifugation time of microhematocrit tubes to obtain accurate results of packed cell volume and total solids in donkeys, dogs, sheep, and cows. [PDF]
Oliva D +6 more
europepmc +1 more source
Hydrogen, Bicarbonate, and Their Associated Exchangers in Cell Volume Regulation. [PDF]
Li Y, Zhou X, Sun SX.
europepmc +1 more source
Acute caffeine treatment protects the developing retina from ischemia‐induced cell death
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento +6 more
wiley +1 more source
Do mechanosensitive Piezo channels set in motion cell volume regulation? [PDF]
Mongin AA.
europepmc +1 more source
Membrane Localization of Piezo1 in the Context of Its Role in the Regulation of Red Blood Cell Volume. [PDF]
Božič B, Svetina S.
europepmc +1 more source
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero +5 more
wiley +1 more source
Cell crowding activates pro-invasive mechanotransduction pathway in high-grade DCIS via TRPV4 inhibition and cell volume reduction. [PDF]
Bu X +9 more
europepmc +1 more source
Cell volume restriction by mercury chloride reduces M1-like inflammatory response of bone marrow-derived macrophages. [PDF]
Chuang YC +5 more
europepmc +1 more source

