Results 11 to 20 of about 1,723 (195)
Designing chimeric enzymes inspired by fungal cellulosomes
Synthetic and Systems Biotechnology, 2020 Cellulosomes are synthesized by anaerobic bacteria and fungi to degrade lignocellulose via synergistic action of multiple enzymes fused to a protein scaffold.
Sean P. Gilmore +4 more
doaj +3 more sources
Acta Biologica SlovenicaCellulosomes are multienzyme complexes originally found on the surface of certain anaerobic celluloytic bacteria and fungi that are specialized in the degradation of plant cell walls.
Maša Vodovnik
doaj +3 more sources
Comparison of the mesophilic cellulosome‐producing Clostridium cellulovorans genome with other cellulosome‐related clostridial genomes [PDF]
Microbial Biotechnology, 2010 Summary Clostridium cellulovorans , an anaerobic and mesophilic bacterium, degrades native substrates in soft biomass such as corn fibre and rice straw efficiently by producing an extracellular enzyme complex called the cellulosome. Recently, we have reported the whole‐genome sequence of
Yutaka Tamaru +6 more
openalex +5 more sources
Combinatorial optimization of the hybrid cellulase complex structure designed from modular libraries [PDF]
Scientific ReportsCellulase selectively recognizes cellulose surfaces and cleaves their β-1,4-glycosidic bonds. Combining hydrolysis using cellulase and fermentation can produce alternative fuels and chemical products.
Hikaru Nakazawa +5 more
doaj +2 more sources
Editorial: Recent advances in cellulosomes and their application in bioenergy production [PDF]
Frontiers in MicrobiologyMarimuthu Anandharaj +3 more
doaj +2 more sources
Affinity-induced covalent protein-protein ligation via the SpyCatcher-SpyTag interaction
Green Carbon, 2023 Production of economically viable bioethanol is potentially an environmentally and financially worthwhile endeavor. One major source for fermentable sugars is lignocellulose. However, lignocellulosic biomass is difficult to degrade, owing to its inherent
Jacob O. Fierer +6 more
doaj +1 more source
Designer Glycolysomes: Colocalisation of Glycolytic Enzymes on a Cellulosome-Based Synthetic Protein Scaffold. [PDF]
Microb BiotechnolThe first three Escherichia coli glycolytic enzymes were converted to the docking enzyme mode and subsequently scaffolded. Increasing copies of the rate‐limiting enzyme boosted the overall performance. At lower enzyme densities, the proximity effects provided by scaffold‐colocalisation result in a higher product yield compared to free docking enzymes ...
Elias M +4 more
europepmc +2 more sources
Cellulosomes from Mesophilic Bacteria [PDF]
Journal of Bacteriology, 2003 Plant cell wall-degrading enzymes have become increasingly important, since the development of efficient biomass degradation methods and the conversion of sugars to valuable products such as butanol and amino acids and utilizable forms of energy such as ethanol and methane could lead to less dependence on imported petroleum as a fuel and chemical ...
Roy H. Doi +4 more
openalex +3 more sources
Combinatorial assembly and optimisation of designer cellulosomes: a galactomannan case study
Biotechnology for Biofuels and Bioproducts, 2022 Background Designer cellulosomes are self-assembled chimeric enzyme complexes that can be used to improve lignocellulosic biomass degradation. They are composed of a synthetic multimodular backbone protein, termed the scaffoldin, and a range of different
Julie Vanderstraeten +8 more
doaj +1 more source