Sequence features and transcriptional stalling within centromere DNA promote establishment of CENP-A chromatin. [PDF]
PLoS Genetics, 2015 Centromere sequences are not conserved between species, and there is compelling evidence for epigenetic regulation of centromere identity, with location being dictated by the presence of chromatin containing the histone H3 variant CENP-A.
Sandra Catania +2 more
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Regulation of Budding Yeast CENP-A levels Prevents Misincorporation at Promoter Nucleosomes and Transcriptional Defects. [PDF]
PLoS Genetics, 2016 The exclusive localization of the histone H3 variant CENP-A to centromeres is essential for accurate chromosome segregation. Ubiquitin-mediated proteolysis helps to ensure that CENP-A does not mislocalize to euchromatin, which can lead to genomic ...
Erica M Hildebrand, Sue Biggins
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Farnesyl Transferase Inhibitors Block the Farnesylation of CENP-E and CENP-F and Alter the Association of CENP-E with the Microtubules [PDF]
Journal of Biological Chemistry, 2000 Human tumor cell lines that are sensitive to the effects of farnesyl transferase inhibitors accumulate in G(2) --> M (except for cells with an activated Ha-ras that accumulate in G(1)). A search for CAAX box proteins from Swiss-Prot revealed more than 300 peptides. Of these, the centromeric proteins CENP-E and CENP-F are preferentially expressed during
H R, Ashar +7 more
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DNA content of a functioning chicken kinetochore [PDF]
, 2014 In order to understand the three-dimensional structure of the functional kinetochore in vertebrates, we require a complete list and stoichiometry for the protein components of the kinetochore, which can be provided by genetic and proteomic experiments ...
Vagnarelli, Paola +5 more
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CENP-C recruits M18BP1 to centromeres to promote CENP-A chromatin assembly [PDF]
Journal of Cell Biology, 2011 Eukaryotic chromosomes segregate by attaching to microtubules of the mitotic spindle through a chromosomal microtubule binding site called the kinetochore. Kinetochores assemble on a specialized chromosomal locus termed the centromere, which is characterized by the replacement of histone H3 in centromeric nucleosomes with the essential histone H3 ...
Moree, Ben +3 more
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DC-FCCS of EGFP-(s)-CENP-T and mCherry-(s)-CENP-S.
, 2018 A) Displayed are G versus lag time. Red: FCS- or autocorrelation-curve G (τ) for mCherry, green: FCS- or autocorrelation-curve G (τ) for EGFP, black: cross-correlation-curve G (τ), AC = autocorrelation.
Sibel Yavas (4915420) +3 more
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HJURP Involvement in De Novo CenH3CENP-A and CENP-C Recruitment
Cell Reports, 2015 Although our understanding of centromere maintenance, marked by the histone H3 variant CenH3CENP-A in most eukaryotes, has progressed, the mechanism underlying the de novo formation of centromeres remains unclear.
Hiroaki Tachiwana +5 more
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EWSR1 maintains centromere identity
Cell Reports, 2023 Summary: The centromere is essential for ensuring high-fidelity transmission of chromosomes. CENP-A, the centromeric histone H3 variant, is thought to be the epigenetic mark of centromere identity.
Risa Kitagawa +4 more
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CENP-A overexpression is associated with shorter mitosis duration.
, 2019 A. Immunoblot showing CENP-A levels in different cell lines. CENP-A antibodies detect endogenous and tagged CENP-A. GFP-CENP-A and SNAP-CENP-A are under the constitutive Copia promoter; CENP-A-GFP was induced with 10 μM CuSO4 for 2 h.
Sylvia Erhardt (7430564) +3 more
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CENP-A loading on centromeres is extremely sensitive to partial PLK1 inhibition.
, 2023 A, Immunofluorescence in RPE-1 cells treated for 72 h with the indicated concentrations of BI2536. CENP-A levels were measured in the DAPI-stained areas (inside yellow dotted lines). B. Quantifications as illustrated in A.
Jasmin Coulombe-Huntington (126532) +7 more
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