Results 61 to 70 of about 98,093 (226)

Cell Cycle‐Specific Regulation of Centrosome Clustering Dynamics in Cancer Cells by the Multifunctional Kinesin HSET

Advanced Science, EarlyView.
CDK5RAP2 associates with the kinesin HSET and forms co‐condensates. HSET motors drive the directional transport of CDK5RAP2 condensates toward microtubule minus ends. During mitosis, HSET self‐assembly further stabilizes clustered centrosomes, thereby enabling pseudo‐bipolar spindle formation in cancer cells with supernumerary centrosomes. Upon mitotic
Po‐Pang Chen, Athira Saju, Chia‐Chou Wu, Tzu‐Han Weng, Su‐Yi Tsai, Tzu‐Lun Huang, Jia‐Ying Su, Chien‐Ling Lin, Yu‐Chun Lin, See‐Yeun Ting, Sheng‐hong Chen, Kuo‐Chiang Hsia   +11 more
wiley   +1 more source

Cortical Dynein Drives Centrosome Clustering in Cells with Centrosome Amplification

Molecular Biology of the Cell, 2022
AbstractDuring cell division, the microtubule nucleating and organizing organelle, known as the centrosome, is critical for the formation of the mitotic spindle. In cells with two centrosomes, each centrosome functions as an anchor point for microtubules, leading to the formation of a bipolar spindle and progression through a bipolar cell division ...
Dayna L. Mercadante, William A. Aaron, Sarah D. Olson, Amity L. Manning   +3 more
openaire   +2 more sources

Dual roles of CCDC102A in governing centrosome duplication and cohesion

Cell Reports
Summary: In animal cells, the dysregulation of centrosome duplication and cohesion maintenance leads to abnormal spindle assembly and chromosomal instability, contributing to developmental disorders and tumorigenesis.
Tianning Wang, Guiliang Fan, Yuqing Xia, Yuhong Zou, Yunjie Liu, Jiaxin Wang, Yingchun Hu, Junlin Teng, Ning Huang, Jianguo Chen   +9 more
doaj   +1 more source

Examination of actin and microtubule dependent APC localisations in living mammalian cells [PDF]

, 2006
(provisional) Background The trafficking of the adenomatous polyposis coli (APC) tumour suppressor protein in mammalian cells is a perennially controversial topic. Immunostaining evidence for an actin-associated APC localisation at intercellular
Adams, A., Askham, J.M., Langford, K.J., Lee, T., Morrison, E.E.   +4 more
core   +3 more sources

Directed Evolution Improves the Catalytic Efficiency of APEX2‐Mediated Proximity‐Dependent RNA Labeling

Advanced Science, EarlyView.
ABSTRACT Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin‐aniline (Btn‐An) remains suboptimal. To overcome this limitation, we performed yeast surface display‐based directed evolution to enhance APEX2 ...
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
wiley   +1 more source

CCDC102B functions in centrosome linker assembly and centrosome cohesion [PDF]

Journal of Cell Science, 2018
ABSTRACT The proteinaceous centrosome linker is an important structure that allows the centrosome to function as a single microtubule-organizing center (MTOC) in interphase cells. However, the assembly mechanism of the centrosome linker components remains largely unknown.
Yuqing Xia, Ning Huang, Zhiquan Chen, Fangyuan Li, Guiliang Fan, Dandan Ma, Jianguo Chen, Junlin Teng   +7 more
openaire   +2 more sources

The G₁ phase Cdks regulate the centrosome cycle and mediate oncogene-dependent centrosome amplification

Cell Division, 2011
Because centrosome amplification generates aneuploidy and since centrosome amplification is ubiquitous in human tumors, a strong case is made for centrosome amplification being a major force in tumor biogenesis.
Saavedra Harold I, Adon Arsene M, Harrison Mary K   +2 more
doaj   +1 more source

LIM kinase1 regulates mitotic centrosome integrity via its activity on dynein light intermediate chains [PDF]

Open Biology, 2018
Abnormal centrosome number and function have been implicated in tumour development. LIM kinase1 (LIMK1), a regulator of actin cytoskeleton dynamics, is found to localize at the mitotic centrosome. However, its role at the centrosome is not fully explored.
Sirong Ou, Mei-Hua Tan, Ting Weng, HoiYeung Li, Cheng-Gee Koh   +4 more
doaj   +1 more source

Chromosomal instability in untreated primary prostate cancer as an indicator of metastatic potential. [PDF]

, 2020
BackgroundMetastatic prostate cancer (PC) is highly lethal. The ability to identify primary tumors capable of dissemination is an unmet need in the quest to understand lethal biology and improve patient outcomes.
Cadaneanu, Radu M, Freeman, Michael R, Garraway, Isla P, Grasso, Catherine S, Hodge, Jennelle, Kim, Minhyung, Knudsen, Beatrice S, Kwan, Lorna, Lewis, Michael S, Li, Xinmin, Liu, Sandy T, Miller, Eric T, Quist, Michael J, Yoon, Junhee, You, Sungyong   +14 more
core  

Centrosome maturation requires YB-1 to regulate dynamic instability of microtubules for nucleus reassembly [PDF]

, 2015
Microtubule formation from the centrosome increases dramatically at the onset of mitosis. This process is termed centrosome maturation. However, regulatory mechanisms of microtubule assembly from the centrosome in response to the centrosome maturation ...
Asaka Masamitsu N., Kawaguchi Atsushi, Matsumoto Ken, Nagata Kyosuke, 川口 敦史, 永田 恭介   +5 more
core   +1 more source