Results 71 to 80 of about 29,683 (253)

Quantitative characterization of tumor cell-free DNA shortening

BMC Genomics, 2020
Background Previous studies found that cell-free DNA (cfDNA) generated from tumors was shorter than that from healthy cells, and selecting short cfDNA could enrich for tumor cfDNA and improve its usage in early cancer diagnosis and treatment monitoring ...
Juntang Guo, Kefeng Ma, Hua Bao, Xiangyuan Ma, Yang Xu, Xue Wu, Yang W. Shao, Mei Jiang, Jin Huang   +8 more
doaj   +1 more source

VarDict: a novel and versatile variant caller for next-generation sequencing in cancer research [PDF]

, 2016
Accurate variant calling in next generation sequencing (NGS) is critical to understand cancer genomes better. Here we present VarDict, a novel and versatile variant caller for both DNA- and RNA-sequencing data.
Ahdesmaki, Miika, Barrett, J. Carl, Chapman, Brad, Dougherty, Brian, Dry, Jonathan R., Hofmann, Oliver, Johnson, Justin, Lai, Zhongwu, Markovets, Aleksandra, McEwen, Robert   +9 more
core   +2 more sources

SDS‐CRISPR for Single‐Nucleotide Variant Detection

Advanced Science, EarlyView.
Structure‐disruption‐sensitive CRISPR (SDS‐CRISPR) converts structural instability into single‐nucleotide precision, thereby overcoming mismatch tolerance in canonical Cas12a and enabling versatile diagnostics across DNA, RNA, and microRNA targets. When applied to rapid IDH1 mutation detection for glioma genotyping and integrated with lateral‐flow ...
Xin Guan, Chong Guo, Jiongyu Zhang, Rui Yang, Yerramsetti Ramachandra, Chengyu Hou, Minjie Pei, Shuo Zhang, Kurt T. Schalper, Xingye Liu, Qian Wu, Ketan R. Bulsara, Changchun Liu   +12 more
wiley   +1 more source

Value of altered methylation patterns of genes RANBP3, LCP2 and GRAP2 in cfDNA in breast cancer diagnosis [PDF]

Journal of Medical Biochemistry
Background: The purpose of this study was to investigate the potential of plasma cfDNA methylation patterns in reflecting tumour methylation changes, focusing on three candidate sites, cg02469161, cg11528914, and cg20131654. These sites were selected for
Hu Qin, Mao Yu, Lan Haomiao, Wei Yi, Chen Yuehua, Ye Qiang, Che Hongying   +6 more
doaj   +1 more source

T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids. [PDF]

, 2017
Body fluid DNA sequencing is a powerful noninvasive approach for the diagnosis of genetic defects, infectious agents and diseases. The success relies on the quantity and quality of the DNA samples.
Chen, Chien-Jen, Chen, Tzu-Han, Chiu, Kuo Ping, Huang, Yu-Feng, Midha, Mohit K, Nai, Yu-Shin, Shen, Chen-Yang, Shiau, Hsin-Chieh, Yu, Alice L   +8 more
core  

Circulating Tumor Cells in Multiple Myeloma: From Peripheral Clues to Central Insights

American Journal of Hematology, EarlyView.
CTC offer a minimally invasive widow into systemic myeloma biology, overcoming the sampling bias of bone marrow biopsies. Their prognostic value at diagnosis, potential role in MRD monitoring, and ability to capture clonal evolution highlight them as actionable biomarkers for future precision medicine.
Benjamin Podvin, Bruno Paiva, Irene M. Ghobrial, Salomon Manier   +3 more
wiley   +1 more source

Characterizing the molecular composition and diagnostic potential of Mycobacterium tuberculosis urinary cell-free DNA using next-generation sequencing

International Journal of Infectious Diseases, 2021
Background: Urine cell-free DNA (cfDNA) is an attractive target for diagnosing pulmonary Mycobacterium tuberculosis (MTB) infection, but has not been thoroughly characterized as a biomarker.
Amy Oreskovic, Adam Waalkes, Elizabeth A. Holmes, Christopher A. Rosenthal, Douglas P.K. Wilson, Adrienne E. Shapiro, Paul K. Drain, Barry R. Lutz, Stephen J. Salipante   +8 more
doaj   +1 more source

Validation of a blood plasma separation system by biomarker detection [PDF]

, 2010
A microfluidic system was developed for blood plasma separation at high flow rate. This system uses only hydrodynamic forces to separate plasma from whole blood.
Campbell, Colin J., Desmulliez, Marc P. Y., Dhariwal, Resham S., Kavanagh, Deirdre M., Kersaudy-Kerhoas, Maiwenn   +4 more
core   +1 more source

Post CAR‐T Measurable Residual Disease Monitoring in Mantle Cell Lymphoma Enables Early Detection of Disease Relapse

American Journal of Hematology, EarlyView.
ABSTRACT CD19‐directed chimeric antigen receptor (CAR) T‐cell therapy has transformed outcomes for patients with relapsed or refractory (r/r) mantle cell lymphoma (MCL), yet more than 40% relapse within one year. Early identification of patients at risk for progression could inform post CAR‐T surveillance and consolidation strategies.
Snegha Ananth, Neha Agarwal, Bita Sahaf, Allison Jacob, Lik Wee Lee, Heidi Simmons, Ilan Kirsch, Sushma Bharadwaj, Wen‐kai Weng, Melody Smith, Saurabh Dahiya, David Miklos, Matthew J. Frank   +12 more
wiley   +1 more source

Epigenetic Signatures in Ovarian Cancer to Determine Potential Diagnostic/Prognostic Biomarkers

Experimed
Objective: Identification of methylation patterns in cell-free DNA (cfDNA) provides a non-invasive methodology for discovering critical biomarkers that facilitate detection and prognostic evaluation of ovarian cancer (OC).
Tuba Gunel, Maurice Jansen, Samet Topuz, Jean Helmijr, Servet Tunoğlu, Tugce Senturk Kirmizitas   +5 more
doaj   +1 more source