Results 61 to 70 of about 23,420 (253)

Direct and indirect method for cfDNA quantification.

, 2019
Schematic presentation of the two methods tested for cell free DNA (cfDNA) assessment in urine samples. (A.) A direct method with simply centrifugation and (B.) an indirect method with cfDNA enrichment based on cfDNA extraction using magnetic beads.
Polona Rajar (5496146), Ola Didrik Saugstad (8207547), Rønnaug Solberg (252034), Monica Åsegg-Atneosen (6008963), Lars Oliver Baumbusch (8207550)   +4 more
core   +1 more source

Plasma cfDNA for the Diagnosis and Prognosis of Colorectal Cancer

Journal of Oncology, 2022
Objective. To evaluate the value of cell-free DNA (cfDNA) for the diagnosis and prognosis of colorectal cancer (CRC). Methods. Peripheral blood specimens of 120 CRC patients and 90 healthy volunteers (as a control cohort) were extracted. A quantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine the cfDNA expression ...
Zhiwei Wu, Lijiang Yu, Juan Hou, Lihua Cui, Yufeng Huang, Qing Chen, Yan Sun, Wangkun Lu, Chenggong Zhang, Di Sun   +9 more
openaire   +2 more sources

ESR1 methylation and ESR1 mutations in circulating tumor cells (CTCs) and paired plasma‐cfDNA of advanced breast cancer patients: A feasibility proof‐of‐concept study

Molecular Oncology, EarlyView.
Circulating tumor cells (CTCs) and plasma cell‐free DNA (cfDNA) were analyzed to detect ESR1 mutations and methylation in patients with advanced breast cancer. CTC‐derived DNA showed higher sensitivity for mutation detection and revealed complementary genetic and epigenetic alterations, highlighting the added value of CTC analysis for understanding ...
Dimitra Stergiopoulou, Athina Markou, Eleonora Nicolo, Mara S Serafini, Qiang Zhang, Youbin Zhang, Lorenzo Gerratana, Andrew A. Davis, Huiping Liu, William J Gradishar, Carolina Reduzzi, Massimo Cristofanilli, Evi Lianidou   +12 more
wiley   +1 more source

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

Longitudinal genome‐wide aneuploidy measurements in circulating cell‐free DNA to predict lack of benefit from pembrolizumab in patients with metastatic urothelial cancer

Molecular Oncology, EarlyView.
Many patients with urothelial cancer do not benefit from treatment with pembrolizumab, while at risk of severe side effects. Changes in the levels of circulating tumor DNA early during treatment, measured by a simple and affordable assay that can be easily implemented in the clinic, can be used as a prognostic tool to identify these patients.
Youssra Salhi, Stavros Makrodimitris, Sandra van Wilpe, Iris te Paske, Vanja de Weerd, Corine M. Beaufort, Hans M. Westgeest, Jens Voortman, Maureen J. B. Aarts, Maud Rijnders, Astrid A. M. van der Veldt, Ronald de Wit, Niven Mehra, Saskia M. Wilting, Debbie G. J. Robbrecht   +14 more
wiley   +1 more source

cfDNA as a biomarker [PDF]

, 2018
Od otkrića cfDNA (od eng. cell-free DNA) do danas, razmatrane su i istraživane mnoge njene primjene u molekularnoj medicini: od dijagnoze i praćenja tumora te predviđanja ozdravljenja pomoću specifičnih biomarkera, do otkrivanja i predviđanja poteškoća u
Tandarić, Luka
core  

cfDNA in pancreatic neuroendocrine carcinoma management with Cushing’s syndrome

, 2021
International audienceSummary We report a case of metastatic pancreatic neuroendocrine carcinoma associated with paraneoplastic Cushing’s syndrome, successively treated with five lines of treatment (platin-etoposide, LV5FU2-dacarbazine, FOLFIRINOX ...
Gauthier, Arnaud, Lopez, Jonathan, Lasolle, Helene, Chardon, Laurence, Barthelemy, David, Gibert, Benjamin, Payen, Léa, Raverot, Gérald, Gerard, Laura, Walter, Thomas, Hervieu, Valerie, Garcia, Jessica   +11 more
core   +1 more source

The significance of serum sST2 and cfDNA in children with severe pneumonia complicated by myocardial damage [PDF]

Journal of Medical Biochemistry
Background: The paper aimed to explore the significance of serum soluble ST2 (sST2) and circulating cell-free DNA (cfDNA) in predicting cardiac functions in children with severe pneumonia complicated by myocardial damage.
Zhao Tingting, Liu Ye, Jia Haoran, Wang Dexing, Du Meng, Wang Weiwei   +5 more
doaj   +1 more source

Relationship between plasma circulating cell-free DNA concentration and treatment outcomes including prognosis in patients with advanced non-small cell lung cancer

BMC Pulmonary Medicine, 2023
Background Some research found that elevated plasma cell-free DNA (cfDNA) concentrations and poor prognosis are associated in non-small cell lung cancer (NSCLC). However, more studies need to be carried out to verify this conclusion.
Wei-Wei Peng, Ying Liu, Huan-Huan Sha, Shao-Di Wen, Ying Fang, Guo-Ren Zhou   +5 more
doaj   +1 more source

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source