Results 51 to 60 of about 29,683 (253)

Liquid biopsy genotyping in lung cancer: ready for clinical utility? [PDF]

, 2017
Liquid biopsy is a blood test that detects evidence of cancer cells or tumor DNA in the circulation. Despite complicated collection methods and the requirement for technique-dependent platforms, it has generated substantial interest due, in part, to its ...
Chen, Yi-Lin, Ho, Chung-Liang, Huang, Wei-Lun, Lin, Chien-Chung, Su, Wu-Chou, Wei, Fang, Wong, David T, Yang, Szu-Chun   +7 more
core   +1 more source

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source

Hijacking emergency granulopoiesis: Neutrophil ontogeny and reprogramming in cancer

Molecular Oncology, EarlyView.
Neutrophils are highly plastic innate immune cells; their functions in cancer extend beyond the tumour microenvironment. This Review summarises current understanding of neutrophil maturation and heterogeneity and highlights tumour‐induced granulopoiesis as a systemic programme that expands immature, immunosuppressive neutrophils via tumour‐derived ...
Gabriela Marinescu, Yi Feng
wiley   +1 more source

Longitudinal circulating tumor DNA profiling in patients with advanced endometrial cancer using an off‐the‐shelf targeted NGS panel

Molecular Oncology, EarlyView.
Intratumour heterogeneity complicates precision management of advanced endometrial cancer. Circulating tumor DNA (ctDNA) offers a minimally invasive strategy to capture tumor evolution and therapeutic resistance. Here, we compare tumor‐agnostic NGS with tumor‐informed ddPCR, outlining their relative sensitivity, concordance, and clinical implications ...
Carlos Casas‐Arozamena, Karin Teien Lande, Eva Diaz, Ana Vilar, Juan Cueva, Efigenia Arias, Victoria Sampayo, Alicia Abalo, Nerea González, Eva Colás, Antonio Gil‐Moreno, Miguel Abal, Gema Moreno‐Bueno, Therese Sørlie, Kristina Lindemann, Laura Muinelo‐Romay   +15 more
wiley   +1 more source

Decreased serum cell-free DNA levels in rheumatoid arthritis [PDF]

, 2015
Purpose: Recent studies have demonstrated that serum/plasma DNA and RNA molecules in addition to proteins can serve as biomarkers. Elevated levels of these nucleic acids have been found not only in acute, but also in chronic conditions, including ...
A Rhodes, C Chang, C Hitchon, D Rooy de, E Bartoloni, F D’Auria, G Perkins, G Steiner, H Schwarzenbach, I Gornik, I Shakhmaeva, J Beck, K Hyrich, K Ohmura, M Galeazzi, M Jørgensen, M Napirei, M Sjoholm, M Smith, M Stroun, N Umetani, P Szodoray, R Landewe, S Chitrabamrung, S Dawson, S Geiger, S Leon, S Niienhuis, S Seegobin, T Rainer, V Gauthier, WJ Venrooij van, X Zhong, Y Lo, Y Lui, Z Huang, Z Smoleńska   +36 more
core   +10 more sources

Relationship between plasma circulating cell-free DNA concentration and treatment outcomes including prognosis in patients with advanced non-small cell lung cancer

BMC Pulmonary Medicine, 2023
Background Some research found that elevated plasma cell-free DNA (cfDNA) concentrations and poor prognosis are associated in non-small cell lung cancer (NSCLC). However, more studies need to be carried out to verify this conclusion.
Wei-Wei Peng, Ying Liu, Huan-Huan Sha, Shao-Di Wen, Ying Fang, Guo-Ren Zhou   +5 more
doaj   +1 more source

Predicting lung cancer recurrence from circulating tumour DNA. Commentary on 'Phylogenetic ctDNA analysis depicts early-stage lung cancer evolution' [PDF]

, 2017
No abstract ...
Daniel J Murphy, HG Colt, J Mazieres, JC Wan, JP Pignon, K Suzawa, Kevin G Blyth, L Calman, M Jamal-Hanjani, M Schuler, TD Crabtree, WC Hanna   +11 more
core   +1 more source

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

Clinical relevance of cell-free DNA quantification and qualification during the first month after lung transplantation

Frontiers in Immunology, 2023
BackgroundMany studies have reported the relevance of donor-derived cfDNA (dd-cfDNA) after lung transplantation (LTx) to diagnose and monitor acute rejection (AR) or chronic rejection or infection (INF).
Pascal Pedini, Pascal Pedini, Benjamin Coiffard, Nicem Cherouat, Sylvia Casas, Frédéric Fina, Audrey Boutonnet, Jean Baptiste Baudey, Printil Aho, Agnes Basire, Sophie Simon, Coralie Frassati, Jacques Chiaroni, Jacques Chiaroni, Martine Reynaud-Gaubert, Christophe Picard, Christophe Picard   +16 more
doaj   +1 more source

Cell-free DNA and outcome in sepsis. [PDF]

, 2012
Severe sepsis can be a catastrophic condition that is often associated with poor outcomes. The early diagnosis and management of the condition are vital in order to improve the chances of survival. However, owing to the syndromal nature of its definition
Cecconi, M, Rhodes, A
core   +1 more source