Results 31 to 40 of about 19,806 (216)

Single‐cell DNA methylation profiling: Technologies, computation, and applications in precision oncology

Molecular Oncology, EarlyView.
Single‐cell DNA methylation (scDNAme) profiling maps epimutational clonal evolution, revealing mechanisms of malignancy and therapeutic resistance across diverse cancer types. By providing a high‐resolution landscape of intratumoral heterogeneity, these technologies empower precise patient stratification, guide the development of enhanced ...
Ik Soo Kim
wiley   +1 more source

Value of altered methylation patterns of genes RANBP3, LCP2 and GRAP2 in cfDNA in breast cancer diagnosis [PDF]

Journal of Medical Biochemistry
Background: The purpose of this study was to investigate the potential of plasma cfDNA methylation patterns in reflecting tumour methylation changes, focusing on three candidate sites, cg02469161, cg11528914, and cg20131654. These sites were selected for
Hu Qin, Mao Yu, Lan Haomiao, Wei Yi, Chen Yuehua, Ye Qiang, Che Hongying   +6 more
doaj   +1 more source

Endogenous cell-free DNA in fetal bovine serum introduces artifacts to in vitro cell-free DNA models

BioTechniques, 2022
Cell-free DNA (cfDNA) is of growing clinical and research significance. In vitro cfDNA models are a useful tool in cfDNA research; however, artifacts in these models may have implications for the interpretation of new and published data.
Bonnita Werner, Kristina Warton, Caroline E Ford   +2 more
doaj   +1 more source

Performance of prenatal cfDNA screening for sex chromosomes

Genetics in Medicine, 2023
The aim of this study was to assess the performance of cell-free DNA (cfDNA) screening to detect sex chromosome aneuploidies (SCAs) in an unselected obstetrical population with genetic confirmation.This was a planned secondary analysis of the multicenter, prospective SNP-based Microdeletion and Aneuploidy RegisTry (SMART) study.
Kimberly Martin, Pe’er Dar, Cora MacPherson, Melissa Egbert, Zachary Demko, Sheetal Parmar, Katelyn Hashimoto, Sina Haeri, Fergal Malone, Ronald J. Wapner, Ashley S. Roman, Asma Khalil, Revital Faro, Rajeevi Madankumar, Noel Strong, Robert M. Silver, Nidhi Vohra, Jon Hyett, Matt Rabinowitz, Charlly Kao, Hakon Hakonarson, Bo Jacobsson, Mary E. Norton   +22 more
openaire   +3 more sources

Using cell‐free RNA to identify B‐ and T‐cell clonality for diagnosis and monitoring of B‐ and T‐cell neoplasms

FEBS Open Bio, EarlyView.
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar, Sally Agersborg, Ahmed Charifa, Pooja Phull, Noa Biran, David Vesole, Harsh Parmar, Andrew Pecora, Andrew Ip, Andre Goy, David Siegel, Maher Albitar   +11 more
wiley   +1 more source

LRG1 Drives Pathological Angiogenesis by Disrupting Neutrophil Mitochondrial Homeostasis in Bladder Cancer

Advanced Science, EarlyView.
In bladder cancer, LRG1 binds to ANXA2 to trigger mitochondrial ROS‐dependent NETosis. This pathogenic cascade actively uncouples endothelial‐mural cell interactions, driving profound vascular destabilization. Consequently, targeting the LRG1‐ANXA2 axis attenuates the neutrophil burden and induces structural vascular normalization, offering a powerful ...
Dongshan Chen, Cong Zhang, Song Xue, Yuan Zeng, Haochen Cui, Chenfei Wang, Jiayi Feng, Lei Yan, Yuanwei Zang   +8 more
wiley   +1 more source

UCtracker: A Deep Learning–Based DNA Methylation Model for Noninvasive Diagnosis and Recurrence Surveillance of Urothelial Carcinoma in a Prospective Study

Advanced Science, EarlyView.
We developed UCtracker, a urine DNA methylation–based deep learning model, for noninvasive diagnosis and postoperative surveillance of urothelial carcinoma. UCtracker demonstrates high diagnostic accuracy, robustness at ultralow sequencing depth, early recurrence detection, and dynamic risk‐stratified monitoring of molecular residual disease ...
Shengwei Xiong, Gaojie Li, Yucai Wu, Yuan Liang, Heng Guo, Yu Zhang, Gengyan Xiong, Long Tian, Xin Zhang, Ye Tian, Zhengguo Ji, Bin Guo, Yue Shi, Jian Fan, Zhihua Li, Yanqing Gong, Shiming He, Xuesong Li, Weimin Ci, Liqun Zhou   +19 more
wiley   +1 more source

Comparison of methods for donor-derived cell-free DNA quantification in plasma and urine from solid organ transplant recipients

Frontiers in Genetics, 2023
In allograft monitoring of solid organ transplant recipients, liquid biopsy has emerged as a novel approach using quantification of donor-derived cell-free DNA (dd-cfDNA) in plasma.
Nicholas Kueng, Nicholas Kueng, Séverine Arcioni, Séverine Arcioni, Fanny Sandberg, Fanny Sandberg, Christian Kuhn, Vanessa Banz, Carlo R. Largiadèr, Daniel Sidler, Ursula Amstutz   +10 more
doaj   +1 more source

A Panel of Circulating Exosomal sncRNAs Associated With Lung Cancer Risk up to 10 Years in Advance

Advanced Science, EarlyView.
Lung cancer is often diagnosed too late, and current screening overlooks many people at risk. In a long‐term study of smokers, a panel of small non‐coding RNAs carried in blood exosomes signals elevated lung cancer risk up to ten years before diagnosis, pointing toward a blood‐based tool for earlier risk detection.
Zhuokun Feng, Masaki Nasu, Ayman Abdul‐Ghani, Yu Chen, Shaoqiu Chen, Isam Mohd Ibrahim, Hanqiu Zhang, Lauren Higa, Zitong Gao, Hua Yang, Raphael Bueno, Loïc Le Marchand, Youping Deng   +12 more
wiley   +1 more source

Integrating NIPT and ultrasound for detecting fetal aneuploidies and abnormalities

Journal of Perinatal Medicine
The advent of non-invasive prenatal testing (NIPT) utilizing cell-free fetal DNA (cfDNA) has transformed the landscape of early chromosomal anomaly detection.
Andonotopo Wiku, Bachnas Muhammad Adrianes, Pribadi Adhi, Alamsyah Azis Muhammad, Aldika Akbar Muhammad Ilham, Ernawati, Hariyasa Sanjaya I Nyoman, Putra Wiradnyana Anak Agung Gede, Aldiansyah Dudy, Dewantiningrum Julian, Adi Pramono Mochammad Besari, Wantania John, Sulistyowati Sri, Stanojevic Milan, Kurjak Asim   +14 more
doaj   +1 more source