Results 131 to 140 of about 958,913 (319)

TRPV1 channel is a potential drug discovery channel for epilepsy

Journal of Cellular Neuroscience and Oxidative Stress, 2018
Epilepsy is one of the most frequent and heterogeneous neurological disorders and it is characterized by several disabilities. Epilepsy is affecting about 3% of people worldwide. Current antiepileptic drugs are only effective in 60% of individuals and many drugs can induce several unwanted side effects in patients.
openaire   +3 more sources

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells-3

, 2011
Copyright information:Taken from "TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells"http://www.biomedcentral.com/1471-2202/8/49BMC Neuroscience 2007;8():49-49.Published online 4 ...
Peter König (62403), Vladimir Chubanov (77427), Wolfgang Kummer (77424), Gabriele Krasteva (77423), Thomas Gudermann (77426), Silke Kaske (77422), Thomas Hofmann (77425)   +6 more
core   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

A study of channel geometry - discharge relationships in semi-natural British rivers as a basis for river restoration and management.

, 1999
PhDRiver restoration has developed over the last three decades in the context of a more holistic approach to river management. One of the most important issues facing river managers is the design of river channel dimensions.
Dangerfield, Helen Rebecca
core  

Non-equilibrium stochastic dynamics of open ion channels [PDF]

, 2013
We present and discuss a modified version of reaction rate theory (RRT) to describe the passage of a positive ion through a biological ion channel. It takes explicit account of the non-equilibrium nature of the permeation process. Unlike traditional RRT,
Kaufman, Igor, Khovanov, Igor A., Luchinsky, D. G., Khovanov, I., McClintock, P. V. E., McClintock, Peter V. E., Eisenberg, R. S., Luchinsky, Dmitrii G., Kaufman, I., Tindjong, Rodrigue, Tindjong, R.   +10 more
core  

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Piezo1, a novel therapeutic target to treat pulmonary arterial hypertension

Frontiers in Physiology, 2023
Qifeng Yang, Xuanyi Li, Yue Xing, Yuqin Chen   +3 more
doaj   +1 more source