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International Journal of Molecular Sciences, 2013 Human chorionic gonadotropin beta subunit (CGB) is a marker of pregnancy as well as trophoblastic and nontrophoblastic tumors. CGB is encoded by a cluster of six genes, of which type II genes (CGB3/9, 5 and 8) have been shown to be upregulated in ...
Ewa Nowak-Markwitz +2 more
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Fragmentation of the β-Subunit of Human Chorionic Gonadotropin Produced by Choriocarcinoma*
Endocrinology, 1988When human chorionic gonadotropin (hCG) was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions with dithiothreitol (DTT), a smaller weight material (CTP'), in addition to the beta-subunit, could be detected by Western blot analysis using antiserum for hCG beta-carboxy-terminal peptide (CTP).
R, Nishimura +5 more
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Mass Spectrometric Characterization of the β-Subunit of Human Chorionic Gonadotropin
Journal of Mass Spectrometry, 1997A high-performance liquid chromatographic/electrospray mass spectrometric (HPLC/MS) technique is described for the characterization of the beta-subunit of the glycopeptide human chorionic gonadotropin (hCG). The beta-subunit of hCG was dissociated from the alpha-subunit using 0.1% trifluoroacetic acid (TFA) and separated by reversed-phase HPLC using a ...
C, Liu, L D, Bowers
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The Expression of the Human Chorionic Gonadotropin Beta Subunit Gene Depends on Negative Control
Cell Biochemistry and Function, 1996AbstractTranscription of the human chorionic gonadotropin (hCG) genes begins in the very early embryo stage and decreases or even disappears in nonplacental tissues. We have studied the regulation of hCG‐beta genes by cell fusion and by the reverse transcription‐polymerase chain reaction (RT‐PCR).
Y H, Chen, T M, Chen, C Y, Hsieh
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Biochemistry, 1984
The beta subunit of human chorionic gonadotropin (hCG) contains at its carboxy terminus an extension of 29 amino acids not found in the beta subunits of the other glycoprotein hormones. This region provides the sites of attachment of four serine-linked oligosaccharide chains.
S R, Lentz +3 more
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The beta subunit of human chorionic gonadotropin (hCG) contains at its carboxy terminus an extension of 29 amino acids not found in the beta subunits of the other glycoprotein hormones. This region provides the sites of attachment of four serine-linked oligosaccharide chains.
S R, Lentz +3 more
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Journal of Reproductive Immunology, 1996
The feasibility of producing epitope-specific antigens by mutation of the gene is demonstrated, the aim being to eliminate unwanted surface epitopes yet allowing the natural folding of the protein to maintain the desired epitope(s). The model protein is the beta subunit of human chorionic gonadotropin (hCG beta) which previously has been used as an ...
A M, Jackson +7 more
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The feasibility of producing epitope-specific antigens by mutation of the gene is demonstrated, the aim being to eliminate unwanted surface epitopes yet allowing the natural folding of the protein to maintain the desired epitope(s). The model protein is the beta subunit of human chorionic gonadotropin (hCG beta) which previously has been used as an ...
A M, Jackson +7 more
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Location of major antigenic sites of the β-subunit of human chorionic gonadotropin
Biochemistry, 1978Purified beta subunit of human chorionic gonadotropin (hCG) was partially reduced with beta-mercaptoethanol, carboxymethylated, and digested with chymotrypsin. The peptides were isolated by high-voltage electrophoresis and paper chromatography. Five major disulfide-containing peptides were isolated, and their location in the parent molecule was ...
N, Swaminathan, G D, Braunstein
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Human chorionic gonadotropin: Amino acid sequence of the α and β subunits
Biochemical and Biophysical Research Communications, 1972Summary The linear amino acid sequences of the α and β subunits of human chorionic gonadotropin have been determined. HCG-α has 92 and HCG-β 139 amino acid residues. There is more homology between HCG-α and ovine LH-α than between HCG-β and ovine LH-β.
O P, Bahl +3 more
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The Journal of Clinical Endocrinology & Metabolism, 1980
We have investigated the different forms of immunoreactive hCGβ excreted in the urine of normal volunteers after iv administration of highly purified hCG β-subunit. Chromatography of urine on Sephadex G-100 revealed two discrete peaks ofhCGβ immunoreactivity as measured with antiserum to hCGβ (SB6).
R E, Wehmann, B C, Nisula
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We have investigated the different forms of immunoreactive hCGβ excreted in the urine of normal volunteers after iv administration of highly purified hCG β-subunit. Chromatography of urine on Sephadex G-100 revealed two discrete peaks ofhCGβ immunoreactivity as measured with antiserum to hCGβ (SB6).
R E, Wehmann, B C, Nisula
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Expression of α- and β-human chorionic gonadotropin subunits in cultured human cells
In Vitro Cellular & Developmental Biology, 1990We surveyed several human cell lines for production of alpha- and beta-human chorionic gonadotropin (hCG) under a variety of conditions known to induce gene expression. alpha- and beta-hCG subunits were monitored in culture media by specific radioimmunoassays and were shown to be quite sensitive to serum refeeding and growth state of all cell types ...
S, Goldstein +4 more
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