Chromatin immunoprecipitation assay
BioTechniques, 2004 Association between proteins and DNA is crucial for many vital cellular functions such as gene transcription, DNA replication and recombination, repair, segregation, chromosomal stability, cell cycle progression, and epigenetic silencing. It is important
Partha M. Das +3 more
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Protocol for using heterologous spike-ins to normalize for technical variation in chromatin immunoprecipitation [PDF]
STAR Protocols, 2021 Summary: Quantifying differential genome occupancy by chromatin immunoprecipitation (ChIP) remains challenging due to variation in chromatin fragmentation, immunoprecipitation efficiencies, and intertube variability. In this protocol, we add heterologous
Franziska Greulich +3 more
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An optimized two-step chromatin immunoprecipitation protocol to quantify the associations of two separate proteins and their common target DNA [PDF]
STAR Protocols, 2021 Summary: Sequential chromatin immunoprecipitation (ChIP) is commonly used to investigate DNA-protein and protein-protein interactions to a specific genomic region. However, it can be tricky to achieve a robust and reproducible signal with sequential ChIP.
Lingli He +3 more
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Chromatin Immunoprecipitation [PDF]
Cold Spring Harbor Protocols, 2020 Chromatin immunoprecipitation, commonly referred to as ChIP, is a powerful technique for the evaluation of in vivo interactions of proteins with specific regions of genomic DNA. Formaldehyde is used in this technique to cross-link proteins to DNA in vivo, followed by the extraction of chromatin from cross-linked cells and tissues.
James, DeCaprio, Thomas O, Kohl
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Chromatin composition is changed by poly(ADP-ribosyl)ation during chromatin immunoprecipitation. [PDF]
PLoS ONE, 2012 Chromatin-immunoprecipitation (ChIP) employs generally a mild formaldehyde cross-linking step, which is followed by isolation of specific protein-DNA complexes and subsequent PCR testing, to analyze DNA-protein interactions.
Sascha Beneke +4 more
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STAR Protocols, 2021 Summary: Conventional chromatin immunoprecipitation (ChIP) includes many steps that need to be optimized. Here, we have described a protocol of fractionation-assisted native ChIP (fanChIP) which combines subfractionation and native ChIP to purify protein/
Ryo Miyamoto, Akihiko Yokoyama
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Iyer Laboratory Solid Tissue Chromatin Immunoprecipitation Protocol [PDF]
, 2017 A detailed protocol on how to perform chromatin immunoprecipitation in solid tissue samples.
Hall, Amelia
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p65 Chromatin Immunoprecipitation Protocol
Bio-Protocol, 2013 Chromatin Immunoprecipitation (ChIP) is an important procedure that allows you to verify if a certain protein is physically located at a regulatory region.
Crissy Dudgeon
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The Snail repressor recruits EZH2 to specific genomic sites through the enrollment of the lncRNA HOTAIR in epithelial-to-mesenchymal transition [PDF]
, 2016 The transcription factor Snail is a master regulator of cellular identity and epithelial-to-mesenchymal transition (EMT) directly repressing a broad repertoire of epithelial genes.
A Kuzmichev +65 more
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Identification and characterization of FAM124B as a novel component of a CHD7 and CHD8 containing complex [PDF]
, 2012 BACKGROUND: Mutations in the chromodomain helicase DNA binding protein 7 gene (CHD7) lead to CHARGE syndrome, an autosomal dominant multiple malformation disorder. Proteins involved in chromatin remodeling typically act in multiprotein complexes.
Batsukh, Tserendulam +7 more
core +3 more sources