Protocol to investigate bivalent histone modification dynamics via chromatin immunoprecipitation followed by re-chromatin immunoprecipitation [PDF]
STAR ProtocolsSummary: Epigenetic modifications play key roles in regulating transcription by altering chromatin structure and accessibility in response to internal cues or external stimuli.
Zhengyao Shao, Hong Qiao
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Optimized protocols for chromatin immunoprecipitation of exogenously expressed epitope-tagged proteins [PDF]
STAR Protocols, 2023 Summary: Chromatin immunoprecipitation (ChIP) assay is widely used for investigating the interaction between DNA and DNA-binding proteins such as transcription factors, co-factors, or chromatin-associated proteins.
Wentong Fang, Chengheng Liao, Qing Zhang
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Protocol for chromatin immunoprecipitation of histone modifications in frozen adipose tissue [PDF]
STAR ProtocolsSummary: Chromatin immunoprecipitation (ChIP) combined with sequencing has revolutionized our understanding of gene regulation; however, its application to frozen adipose tissue presents unique challenges due to the high levels of lipid content. Here, we
Akin Cayir +4 more
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Protocol for in vivo chromatin immunoprecipitation on purified chromatin isolated from mouse liver nuclei [PDF]
STAR ProtocolsSummary: Chromatin immunoprecipitation (ChIP) is used to investigate genome binding by transcription factors, but it can be problematic. We present a protocol to isolate fixed DNA-protein complexes from mouse liver prior to chromatin shearing.
Lei Li, May G. Akl, Scott B. Widenmaier
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A low-input high resolution sequential chromatin immunoprecipitation method captures genome-wide dynamics of bivalent chromatin [PDF]
Epigenetics & ChromatinBackground Bivalent chromatin is an exemplar of epigenetic plasticity. This co-occurrence of active-associated H3K4me3 and inactive-associated H3K27me3 histone modifications on opposite tails of the same nucleosome occurs predominantly at promoters that ...
Janith A. Seneviratne +3 more
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An Optimized Chromatin Immunoprecipitation Protocol for Quantification of Protein-DNA Interactions [PDF]
STAR Protocols, 2020 Summary: Transcription factors are important regulators of cell fate and function. Knowledge about where transcription factors are bound in the genome is crucial for understanding their function.
Wim J. de Jonge +3 more
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Protocol for development and use of spike-in control for chromatin immunoprecipitation of chromatin-binding proteins in Schizosaccharomyces pombe [PDF]
STAR ProtocolsSummary: Chromatin immunoprecipitation (ChIP) assays provide quantitative information about the genomic localization of chromatin-binding proteins; however, their sensitivity is limited by several technical variables.
Jasbeer S. Khanduja, Mo Motamedi
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A native chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in strawberry fruits [PDF]
Plant Methods, 2020 Background Covalent modifications of histones and histone variants have great influence on chromatin structure, which is involved in the transcriptional regulation of gene expression.
Xiaorong Huang +4 more
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Nuclear CaMKII enhances histone H3 phosphorylation and remodels chromatin during cardiac hypertrophy. [PDF]
, 2013 Calcium/calmodulin-dependent protein kinase II (CaMKII) plays a central role in pathological cardiac hypertrophy, but the mechanisms by which it modulates gene activity in the nucleus to mediate hypertrophic signaling remain unclear. Here, we report that
An, Woojin +7 more
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Native Chromatin Immunoprecipitation [PDF]
, 2011 Native chromatin immunoprecipitation refers to a method that allows for identification and quantification of DNA that is associated with specific chromatin proteins without altering the structure of these proteins. The method has been used with great success in the past and has some advantages over the more widely used cross-linking chromatin ...
Cosseau, Céline, Grunau, Christoph
openaire +3 more sources