Assessment of sera for chromatin-immunoprecipitation
BioTechniques, 2008 Chromatin-immunoprecipitation (ChIP) is a powerful technique for mapping the protein-DNA interactions that occur in living cells. The critical technical determinant for successful ChIP is the availability of an appropriate, “ChIP-grade” serum.
Juliette Nguyen +3 more
doaj +1 more source
Additional sex combs interacts with enhancer of zeste and trithorax and modulates levels of trimethylation on histone H3K4 and H3K27 during transcription of hsp70. [PDF]
, 2017 BACKGROUND: Maintenance of cell fate determination requires the Polycomb group for repression; the trithorax group for gene activation; and the enhancer of trithorax and Polycomb (ETP) group for both repression and activation.
Brock, Hugh W +4 more
core +2 more sources
RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans
Bio-Protocol, 2014 The RNA chromatin immunoprecipitation assay (RNA-ChIP) allows detection and quantification of RNA–protein interactions using in vivo cross-linking with formaldehyde followed by immunoprecipitation of the RNA–protein complexes.
Germano Cecere, Alla Grishok
doaj +1 more source
Chromatin immunoprecipitation to analyze DNA binding sites of HMGA2. [PDF]
PLoS ONE, 2011 BACKGROUND: HMGA2 is an architectonic transcription factor abundantly expressed during embryonic and fetal development and it is associated with the progression of malignant tumors.
Nina Winter +4 more
doaj +1 more source
The laminA/NF-Y protein complex reveals an unknown transcriptional mechanism on cell proliferation [PDF]
, 2016 Lamin A is a component of the nuclear matrix that also controls proliferation by largely unknown mechanisms. NF-Y is a ubiquitous protein involved in cell proliferation composed of three subunits (-YA -YB -YC) all required for the DNA ...
Alonzi, Tonino +16 more
core +2 more sources
The enzymes LSD1 and Set1A cooperate with the viral protein HBx to establish an active hepatitis B viral chromatin state [PDF]
, 2016 Indexación: Web of ScienceWith about 350 million people chronically infected around the world hepatitis B is a major health problem. Template for progeny HBV synthesis is the viral genome, organized as a minichromosome (cccDNA) inside the hepatocyte ...
Alarcon, V +9 more
core +2 more sources
ChIP on Chip: surprising results are often artifacts
BMC Genomics, 2010 Background The method of chromatin immunoprecipitation combined with microarrays (ChIP-Chip) is a powerful tool for genome-wide analysis of protein binding. However, a high background signal is a common phenomenon.
Skarstad Kirsten, Waldminghaus Torsten
doaj +1 more source
Allele-specific chromatin immunoprecipitation studies show genetic influence on chromatin state in human genome. [PDF]
PLoS Genetics, 2007 Several recent studies have shown a genetic influence on gene expression variation, including variation between the two chromosomes within an individual and variation between individuals at the population level.
Mitsutaka Kadota +7 more
doaj +1 more source
Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq. [PDF]
, 2016 BackgroundThe robustness of ChIP-seq datasets is highly dependent upon the antibodies used. Currently, polyclonal antibodies are the standard despite several limitations: They are non-renewable, vary in performance between lots and need to be validated ...
Busby, Michele +11 more
core +1 more source
TGFβ impairs HNF1α functional activity in Epithelial-to-Mesenchymal Transition interfering with the recruitment of CBP/p300 acetyltransferases [PDF]
, 2019 The cytokine transforming growth factor β (TGFβ) plays a crucial role in the induction of both epithelial-to-mesenchymal transition (EMT) program and fibro-cirrhotic process in the liver, where it contributes also to organ inflammation following several ...
Amicone, Laura +8 more
core +1 more source