Results 11 to 20 of about 1,114,920 (242)

Quantitative evaluation of chromosomal rearrangements in gene-edited human stem cells by CAST-Seq.

Cell Stem Cell, 2021
Genome editing has shown great promise for clinical translation but also revealed the risk of genotoxicity caused by off-target effects of programmable nucleases.
G. Turchiano, G. Andrieux, Julia Klermund, Georges Blattner, V. Pennucci, Melina El Gaz, G. Monaco, Sushmita Poddar, Claudio Mussolino, T. Cornu, M. Boerries, T. Cathomen   +11 more
semanticscholar   +3 more sources

Dynamic large-scale chromosomal rearrangements fuel rapid adaptation in yeast populations. [PDF]

PLoS Genetics, 2013
Large-scale genome rearrangements have been observed in cells adapting to various selective conditions during laboratory evolution experiments. However, it remains unclear whether these types of mutations can be stably maintained in populations and how ...
Shang-Lin Chang, Huei-Yi Lai, Shu-Yun Tung, Jun-Yi Leu   +3 more
doaj   +2 more sources

Chromosomal rearrangements drive diversity in arboreal rodents of the genus Oecomys [PDF]

Scientific Reports
Oecomys (Rodentia, Sigmodontinae, Oryzomyini) is a taxonomically complex and cytogenetically diverse genus with a controversial intraspecific phylogenetic relationship.
Vergiana dos Santos Paixão, Stella Miranda Malcher, Willam Oliveira da Silva, Malcolm Andrew Ferguson-Smith, Patricia Caroline Mary O’Brien, Rogério Vieira Rossi, Julio Cesar Pieczarka, Cleusa Yoshiko Nagamachi   +7 more
doaj   +2 more sources

Precise and Predictable CRISPR Chromosomal Rearrangements Reveal Principles of Cas9-Mediated Nucleotide Insertion.

Molecular Cell, 2018
Chromosomal rearrangements including large DNA-fragment inversions, deletions, and duplications by Cas9 with paired sgRNAs are important to investigate genome structural variations and developmental gene regulation, but little is known about the ...
Jia Shou, Jin-huan Li, Yingbin Liu, Qiang Wu   +3 more
semanticscholar   +3 more sources

In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system

Nature, 2014
Chromosomal rearrangements have a central role in the pathogenesis of human cancers and often result in the expression of therapeutically actionable gene fusions.
Danilo Maddalo, Eusebio Manchado, Carla P. Concepcion, Ciro Bonetti, Joana A. Vidigal, Yoon-Chi Han, Paul Ogrodowski, A. Crippa, N. Rekhtman, E. de Stanchina, S. Lowe, A. Ventura   +11 more
semanticscholar   +3 more sources

Gene editing of NCF1 loci is associated with homologous recombination and chromosomal rearrangements [PDF]

Communications Biology
CRISPR-based genome editing of pseudogene-associated disorders, such as p47 phox -deficient chronic granulomatous disease (p47 CGD), is challenged by chromosomal rearrangements due to presence of multiple targets.
Federica Raimondi, Kah Mun Siow, Dominik Wrona, Carla Fuster-García, Oleksandr Pastukhov, Michael Schmitz, Katja Bargsten, Lucas Kissling, Daan C. Swarts, Geoffroy Andrieux, Toni Cathomen, Ute Modlich, Martin Jinek, Ulrich Siler, Janine Reichenbach   +14 more
doaj   +2 more sources

DNA Double-Strand Breaks Affect Chromosomal Rearrangements during Methotrexate-Mediated Gene Amplification in Chinese Hamster Ovary Cells

Pharmaceutics, 2021
Methotrexate (MTX)-mediated gene amplification has been widely used in Chinese hamster ovary (CHO) cells for the biomanufacturing of therapeutic proteins.
Jong Youn Baik, Hye-Jin Han, Kelvin H. Lee   +2 more
doaj   +1 more source

Identification of a familial complex chromosomal rearrangement by optical genome mapping

Molecular Cytogenetics, 2022
Background Complex chromosomal rearrangements (CCRs) are rare chromosomal structural variations, containing a variety of rearrangements such as translocation, inversion and/or insertion.
Yang Yang, Wang Hao
doaj   +1 more source

Convergent adaptation of Saccharomyces uvarum to sulfite, an antimicrobial preservative widely used in human-driven fermentations.

PLoS Genetics, 2021
Different species can find convergent solutions to adapt their genome to the same evolutionary constraints, although functional convergence promoted by chromosomal rearrangements in different species has not previously been found.
Laura G Macías, Melisa González Flores, Ana Cristina Adam, María E Rodríguez, Amparo Querol, Eladio Barrio, Christian Ariel Lopes, Roberto Pérez-Torrado   +7 more
doaj   +1 more source

Multi-invasions Are Recombination Byproducts that Induce Chromosomal Rearrangements.

Cell, 2017
Aurèle Piazza, W. D. Wright, W. Heyer
semanticscholar   +3 more sources