Results 121 to 130 of about 5,018,281 (306)

Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification.

Proceedings of the National Academy of Sciences of the United States of America, 1989
Several chemical and enzymatic properties were examined in the DNA extracted from dry remains of soft tissues that vary in age from 4 to 13,000 years and represent four species, including two extinct animals (the marsupial wolf and giant ground sloth ...
S. Pääbo
semanticscholar   +1 more source

Detection rate for ESR1 mutations is higher in circulating‐tumor‐cell‐derived genomic DNA than in paired plasma cell‐free DNA samples as revealed by ddPCR

Molecular Oncology, EarlyView.
Analysis of ESR1 mutations in plasma cell‐free DNA (cfDNA) is highly important for the selection of treatment in patients with breast cancer. Using multiplex‐ddPCR and identical blood draws, we investigated whether circulating tumor cells (CTCs) and cfDNA provide similar or complementary information for ESR1 mutations.
Stavroula Smilkou, Aliki Ntzifa, Victoria Tserpeli, Ioanna Balgkouranidou, Alkistis Papatheodoridi, Evangelia Razis, Helena Linardou, Christos Papadimitriou, Amanda Psyrri, Flora Zagouri, Stylianos Kakolyris, Evi Lianidou   +11 more
wiley   +1 more source

Robust acute myeloid leukemia engraftment in humanized scaffolds using injectable biomaterials and intravenous xenotransplantation

Molecular Oncology, EarlyView.
Patient‐derived xenografts (PDXs) can be improved by implantation of a humanized niche. We tested different biomaterials and approaches, and demonstrate that the combination of an injectable biomaterial for scaffold creation plus an intravenous route for acute myeloid leukemia (AML) xenotransplantation provide the most convenient and robust approach to
Daniel Busa, Zdenka Herudkova, Jan Hyl, Jakub Vlazny, Filip Sokol, Kvetoslava Matulova, Adam Folta, Jakub Hynst, Lucy Vojtova, Leos Kren, Martin Repko, Zdenek Racil, Jiri Mayer, Martin Culen   +13 more
wiley   +1 more source

Unified Approach to Universal Cloning and Phase-Covariant Cloning [PDF]

arXiv, 2008
We analyze the problem of approximate quantum cloning when the quantum state is between two latitudes on the Bloch's sphere. We present an analytical formula for the optimized 1-to-2 cloning. The formula unifies the universal quantum cloning (UQCM) and the phase covariant quantum cloning.
arxiv  

High-throughput FastCloning technology: A low-cost method for parallel cloning

PLoS ONE, 2022
FastCloning, a reliable cloning technique for plasmid construction, is a widely used protocol in biomedical research laboratories. Only two-step molecular manipulations are required to add a gene (cDNA) of interest into the desired vector.
Hua Jiang, Fan Meng, Deren Lu, Yanjuan Chen, Guilin Luo, Yuejun Chen, Jun Chen, Cheng Chen, Xi Zhang, Dan Su   +9 more
doaj  

Cloning alphavirus and flavivirus sequences for use as positive controls in molecular diagnostics

Revista Peruana de Medicina Experimental y Salud Pública, 2016
The purpose of the study was to obtain a positive control to validate molecular techniques (reverse transcription– polymerase chain reaction [RT-PCR]) used in the diagnosis and research of viral infections. From strains of Chikungunya virus (CHIKV), Zika
Daría Camacho, Jesús Reyes, Leticia Franco, Guillermo Comach, Elizabeth Ferrer   +4 more
doaj   +1 more source

NMR Experimental Demonstration of Probabilistic Quantum Cloning

, 2011
The method of quantum cloning is divided into two main categories: approximate and probabilistic quantum cloning. The former method is used to approximate an unknown quantum state deterministically, and the latter can be used to faithfully copy the state
Chen, Hongwei, Chong, Bo, Du, Jiangfeng, Lu, Dawei, Peng, Xinhua, Qin, Gan, Zhou, Xianyi   +6 more
core   +1 more source

Accelerated Evolution of the ASPM Gene Controlling Brain Size Begins Prior to Human Brain Expansion [PDF]

, 2004
Primary microcephaly (MCPH) is a neurodevelopmental disorder characterized by global reduction in cerebral cortical volume. The microcephalic brain has a volume comparable to that of early hominids, raising the possibility that some MCPH genes may have ...
Abeysinghe, Aota, Benson, Bernardi, Bond, Bond, Chen, Chenn, Chuzhanova, Clark, Crandall, Dewyse, do Carmo Avides, Duret, Endo, Evans, Galtier, Gould, Hughes, Jurka, Kent, Kouprina, Kreitman, McCreary, McDonald, Mochida, Morgenstern, Noskov, Polushin, Rehen, Riparbelli, Ripoll, Roberts, Rudiger, Sharp, Sharp, Tobias, Wood, Yang, Yang, Zhang   +40 more
core   +3 more sources

TRPM4 contributes to cell death in prostate cancer tumor spheroids, and to extravasation and metastasis in a zebrafish xenograft model system

Molecular Oncology, EarlyView.
Transient receptor potential melastatin‐4 (TRPM4) is overexpressed in prostate cancer (PCa). Knockout of TRPM4 resulted in reduced PCa tumor spheroid size and decreased PCa tumor spheroid outgrowth. In addition, lack of TRPM4 increased cell death in PCa tumor spheroids.
Florian Bochen, Saurav Subedi, Federico La Manna, Sofia Jarrin, Irida Papapostolou, Marianna Kruithof‐de Julio, Christine Peinelt   +6 more
wiley   +1 more source

Infinitary version of Pol-Inv Galois connection [PDF]

arXiv, 2013
In this article, we prove infinitary version of one to one correspondence theorem between clones and relational clones on a fixed possibly infinite set. We also characterize the relational clone corresponding to the clone of all finitary operations. By this characterization, we obtain correspondence between finitary clones on a fixed infinite set and ...
arxiv