Identification of a novel aminoglycoside nucleotidyltransferase gene in Morganella morganii from farm sewage. [PDF]
Lu J+12 more
europepmc +1 more source
High‐Throughput Microfluidic‐Mediated Assembly of Layer‐By‐Layer Nanoparticles
The layer‐by‐layer (LbL) assembly of polymer films on nanoparticle (NP) surfaces is a promising technique for targeted drug delivery. Despite its success in preclinical mouse models, a current good manufacturing practices (cGMP) compliant, clinical‐scale production method has been lacking.
Ivan S. Pires+4 more
wiley +1 more source
Leptin 30 years - A chat with Jeffrey M. Friedman. [PDF]
Moura-Assis A, Velloso LA.
europepmc +1 more source
A Universal Biomimetic Approach for Making Artificial Antigen‐Presenting Cells for T Cell Activation
This study shows a biomimetic silica microcapsule (SMC) fabrication method under mild conditions for making artificial antigen‐presenting cells (aAPCs). Inspired by marine biomineralization, peptide‐mediated biosilicification enables silica shell formation on emulsion templates. The resulting SMCs possess a core–shell structure, controlled fluorescence
Fei Hou+5 more
wiley +1 more source
RecombiCraft library construction: A novel method for DNA library cloning and expansion using non-enzymatic single-step DNA recombination and liquid culture. [PDF]
Kawai-Harada Y+7 more
europepmc +1 more source
Engineering Assembloids to Mimic Graft‐Host Skeletal Muscle Interaction
This study develops a graft‐host skeletal muscle assembloid model combining neuromuscular organoids with tissue‐engineered constructs. Pre‐seeding decellularized muscles with myogenic cells enhances cell migration and axon invasion from the organoid. The model exhibits regenerative capacity following acute damage, advancing the understanding of human ...
Lucia Rossi+13 more
wiley +1 more source
Optimization of handmade cloning enhances pregnancy rates and live calf production in cattle. [PDF]
Su R+8 more
europepmc +1 more source
Short‐chain fatty acids modulate anti‐ROR1 chimeric antigen receptor (CAR) T‐cell function in an intestinal adenocarcinoma‐on‐chip model. Butyrate and propionate reduce cytotoxicity, cytokine release, and infiltration by inducing a regulatory T‐cell phenotype and T‐cell exhaustion via histone deacetylase inhibition.
Valentin D. Wegner+5 more
wiley +1 more source
A PCR-independent, annealing-free cloning method for the insertion of short DNA fragments. [PDF]
Li L+18 more
europepmc +1 more source