Results 261 to 270 of about 822,631 (292)
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Molecular Cloning of Steroid Hydroxylases
Endocrine Research, 1984Recombinant plasmids specific for bovine adrenocortical cytochromes P-450scc and P-45011 beta have been identified and characterized. Using these cDNA inserts as probes, it is found that tissue specificity of gene expression for these two proteins is as expected. Cytochrome P-450scc mRNA is found in adrenocortical and corpus luteum RNA while cytochrome
M E, John +3 more
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Molecular cloning in heterologous systems
1983During the last years the knowledge accumulated by fundamental research of DNA recombination in vitro became more relevant to biotechnology.
K, Esser, C, Lang-Hinrichs
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Molecular Cloning of PCR Products
Current Protocols in Molecular Biology, 2001AbstractIt is often desirable to clone PCR products to establish a permanent source of cloned DNA for hybridization studies, to obtain highâquality DNA sequencing results, or to separate products when PCR amplification yields a complex mixture. The efficiency of direct cloning of PCR products can be improved by generating suitable ends on the amplified
M, Finney, P E, Nisson, A, Rashtchian
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2013
This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
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This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
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Molecular cloning of feline CD34
Veterinary Immunology and Immunopathology, 2003In humans, baboons, dogs and mice CD34 is a cell surface molecule that is expressed on primitive hematopoietic cells and in all these species CD34 positive cells can be used to effect long-term haematopoietic reconstitution. CD34 positive haematopoietic cells therefore provide a convenient and relatively small cell population to target when attempting ...
Yogalingam, G., Anson, D.
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Molecular Cloning of Human Amidophosphoribosyltransferase
Biochemical and Biophysical Research Communications, 1993The cDNA of human amidophosphoribosyltransferase (EC 2.4.2.14, ATase), which is the supposed regulatory allosteric enzyme of de novo purine nucleotide biosynthesis, has been cloned from human hepatoma (HepG2) cDNA library. The predicted open reading frame encodes a protein of 517 amino acids with a deduced molecular weight (Mr) of 57,398, which is ...
H, Iwahana +7 more
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Molecular Cloning in the Streptococci
1982The genus Streptococcus contains a large number of species able to cause infection in humans and in animals. Our research efforts have revolved around the study of streptococci that normally reside in the human oral cavity. In this regard, Streptococcus mutans is thought to play an important role in the etiology of dental caries (tooth decay) in humans
F L, Macrina +3 more
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Sarcolectin: Complete purification for molecular cloning
Biochimie, 1999A great variety of vertebrate cells contain detectable amounts of lectins, able to stimulate the initiation of cellular DNA synthesis. One of them, sarcolectin (SCL) can block interferon (IFN) action, by inhibiting the synthesis and the expression of the IFN dependent secondary proteins.
P H, Jiang, F, Chany-Fournier, C, Chany
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Molecular cloning of mouse thioredoxin reductases
Gene, 2000We screened clones for thioredoxin reductase genes with a degenerate PCR-based strategy and have isolated two novel cDNA clones from a mouse thymocyte cDNA library. These encode two distinct thioredoxin reductases (TrxR1 and TrxR2) with 499 and 527 amino acid (aa) residues and calculated molecular masses of 54.5 kDa and 56.8 kDa respectively.
H, Kawai, T, Ota, F, Suzuki, M, Tatsuka
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Molecular cloning of Drosophila TFIID subunits
Nature, 1994Transcription initiation factor TFIID is a multisubunit complex containing a TATA-box-binding factor (TFIID tau/TBP) and associated polypeptide factors (TAFs) with sizes ranging from M(r) approximately 20,000 to > 200,000. As a result of direct promoter interactions, TFIID nucleates the assembly of RNA polymerase II and other initiation factors into a ...
T, Kokubo +5 more
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