Results 261 to 270 of about 814,547 (292)

Molecular Cloning of HumanFrizzled-6,

Biochemical and Biophysical Research Communications, 1998
The Frizzled genes encode receptors for WNTs, secreted glycoproteins implicated in development as well as in carcinogenesis. In this paper, we report molecular cloning of Hfz6, the human homologue of Mfz6. Nucleotide sequence analysis showed that the Hfz6 gene encodes the 706 amino-acid protein with seven transmembrane domains, a cystein-rich domain in
M, Tokuhara, M, Hirai, Y, Atomi, M, Terada, M, Katoh   +4 more
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Molecular cloning of human RP105

European Journal of Immunology, 1997
AbstractRP105 is a 105‐kDa type I membrane protein of the leucine‐rich repeat (LRR) family. Anti‐RP105 sensitizes B cells to antigen‐receptor‐mediated apoptosis, but protects B cells from radiation‐induced apoptosis and stimulates B cell proliferation. The sequence of the mouse RP105 has been reported.
I, Fugier-Vivier, O, de Bouteiller, C, Guret, F, Fossiez, J, Banchereau, M G, Mattei, S, Aït-Yahia, E, Garcia, S, Lebecque, Y J, Liu   +9 more
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Molecular cloning of the cDNA for ligatin

Journal of Cell Science, 1989
ABSTRACT We describe the first isolation and sequence of a partial cDNA clone encoding ligatin, a trafficking receptor for phosphoglycoproteins. The clone was isolated from a human U937 promonocyte λgtll cDNA library using rabbit antiserum to rat ileal ligatin.
E R, Jakoi, A L, Brown, Y S, Ho, R, Snyderman   +3 more
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Molecular Cloning of Steroid Hydroxylases

Endocrine Research, 1984
Recombinant plasmids specific for bovine adrenocortical cytochromes P-450scc and P-45011 beta have been identified and characterized. Using these cDNA inserts as probes, it is found that tissue specificity of gene expression for these two proteins is as expected. Cytochrome P-450scc mRNA is found in adrenocortical and corpus luteum RNA while cytochrome
M E, John, E R, Simpson, V, Boggaram, M R, Waterman   +3 more
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Molecular Cloning of PCR Products

Current Protocols in Molecular Biology, 2001
AbstractIt is often desirable to clone PCR products to establish a permanent source of cloned DNA for hybridization studies, to obtain high‐quality DNA sequencing results, or to separate products when PCR amplification yields a complex mixture. The efficiency of direct cloning of PCR products can be improved by generating suitable ends on the amplified
M, Finney, P E, Nisson, A, Rashtchian
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Molecular Cloning of Human Amidophosphoribosyltransferase

Biochemical and Biophysical Research Communications, 1993
The cDNA of human amidophosphoribosyltransferase (EC 2.4.2.14, ATase), which is the supposed regulatory allosteric enzyme of de novo purine nucleotide biosynthesis, has been cloned from human hepatoma (HepG2) cDNA library. The predicted open reading frame encodes a protein of 517 amino acids with a deduced molecular weight (Mr) of 57,398, which is ...
H, Iwahana, J, Oka, N, Mizusawa, E, Kudo, S, Ii, K, Yoshimoto, E W, Holmes, M, Itakura   +7 more
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Molecular cloning of feline CD34

Veterinary Immunology and Immunopathology, 2003
In humans, baboons, dogs and mice CD34 is a cell surface molecule that is expressed on primitive hematopoietic cells and in all these species CD34 positive cells can be used to effect long-term haematopoietic reconstitution. CD34 positive haematopoietic cells therefore provide a convenient and relatively small cell population to target when attempting ...
Yogalingam, G., Anson, D.
openaire   +3 more sources

Molecular cloning in heterologous systems

1983
During the last years the knowledge accumulated by fundamental research of DNA recombination in vitro became more relevant to biotechnology.
K, Esser, C, Lang-Hinrichs
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Molecular Cloning of the Thyrotropin Receptor

Science, 1989
The pituitary hormone thyrotropin, or thyroid-stimulating hormone (TSH), is the main physiological agent that regulates the thyroid gland. The thyrotropin receptor (TSHR) was cloned by selective amplification with the polymerase chain reaction of DNA segments presenting sequence similarity with genes for G protein-coupled receptors. Out of
Parmentier, Marc, Libert, Frédérick, Maenhaut, Carine, Lefort, Anne, Gérard, Claude, Perret, Jason, Van Sande, Jacqueline, Dumont, Jacques, Vassart, Gilbert   +8 more
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Molecular Cloning

2013
This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
openaire   +2 more sources