Results 101 to 110 of about 1,786,363 (358)

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Diversity and biosynthetic potential of culturable microbes associated with toxic marine animals [PDF]

, 2013
Tetrodotoxin (TTX) is a neurotoxin that has been reported from taxonomically diverse organisms across 14 different phyla. The biogenic origin of tetrodotoxin is still disputed, however, TTX biosynthesis by host-associated bacteria has been reported.
Chau, Rocky, Kalaitzis, John, Neilan, Brett, Wood, Susanna A.   +3 more
core   +4 more sources

Construction and comparison of different vehicles for heterologous gene expression in Zymomonas mobilis

Microbial Biotechnology
Zymomonas mobilis has the potential to be an optimal chassis for the production of bulk chemicals derived from pyruvate. However, a lack of available standardized and characterized genetic tools hinders both efficient engineering of Z.
Gerrich Behrendt, Maria Vlachonikolou, Helga Tietgens, Katja Bettenbrock   +3 more
doaj   +1 more source

Synergistic Osteogenesis After Co‐Administration of cmRNAs Encoding BMP‐2 and BMP‐7 Utilizing a Transcript‐Activated Matrix

Advanced Functional Materials, EarlyView.
This study demonstrates that the dual delivery of BMP‐2/‐7 coding cmRNAs for bone healing is demonstrated as feasible, safe, and highly osteogenic. Compared to single BMP‐2 or BMP‐7 cmRNAs, the combination enhances the production of both mineral and organic components of the extracellular matrix when delivered using a collagen‐HA scaffold, supporting ...
Claudia Del Toro Runzer, Joanna M. Sadowska, Nadia Roumans, Christian Plank, Christopher H. Evans, Fergal J. O'Brien, Martijn van Griensven, Elizabeth R. Balmayor   +7 more
wiley   +1 more source

A tale of three kingdoms: Members of the Phylum Nematoda independently acquired the detoxifying enzyme cyanase through horizontal gene transfer from plants and bacteria [PDF]

, 2018
Horizontal gene transfer (HGT) has played an important role in the evolution of nematodes. Among candidate genes, cyanase, which is typically found only in plants, bacteria and fungi, is present in more than 35 members of the Phylum Nematoda, but absent ...
Hoberg, E. P, Mitreva, M., Thompson, P., Tuo, W., Tyagi, R., Zarlenga, D. S   +5 more
core   +2 more sources

Nitrogen regulation of protein–protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea [PDF]

, 2013
Gene homologs of GlnK PII regulators and AmtB-type ammonium transporters are often paired on prokaryotic genomes, suggesting these proteins share an ancient functional relationship.
Adler, Allers, Andrade, Atkinson, Bell, Bitan-Banin, Bonete, Camacho, Chellamuthu, Cline, Conroy, Coutts, Detsch, Dodsworth, Eichler, Garber, Gruswitz, Hammelmann, Han, Hervás, Huergo, Huergo, Huergo, Javelle, Javelle, Jiang, Khademi, Lee, Liu, Maier, Mangum, Martínez-Espinosa, Martínez-Espinosa, Moure, Najera-Fernandez, Pedro-Roig, Pedro-Roig, Pedro-Roig, Radchenko, Rodriguez-Valera, Russek, Sant'Anna, Severi, Strösser, Thomas, Thornton, Tremblay, Weiss, Werner, Xu, Zamaroczy, Zhang, Zhang, Zheng   +53 more
core   +2 more sources

Curation of microarray oligonucleotides and corresponding ESTs/cDNAs used for gene expression analysis in zebra finches

BMC Research Notes, 2018
Objectives Zebra finches are a major model organism for investigating mechanisms of vocal learning, a trait that enables spoken language in humans. The development of cDNA collections with expressed sequence tags (ESTs) and microarrays has allowed for ...
Peter V. Lovell, Nicole A. Huizinga, Abel Getachew, Brianna Mees, Samantha R. Friedrich, Morgan Wirthlin, Claudio V. Mello   +6 more
doaj   +1 more source

Next‐Generation Bio‐Reducible Lipids Enable Enhanced Vaccine Efficacy in Malaria and Primate Models

Advanced Functional Materials, EarlyView.
Structure–activity relationship (SAR) optimization of bio‐reducible ionizable lipids enables the development of highly effective lipid nanoparticle (LNP) mRNA vaccines. Lead LNPs show superior tolerability and antibody responses in rodents and primates, outperforming approved COVID‐19 vaccine lipids.
Ruben De Coen, Sabah Kasmi, Shrinivas Dumbre, Lies Baekens, Elise Seynaeve, Louise Van den Hauwe, Niccolò Cantini, Aello Garantoudi, Filipe Branco Madeira, Alain Marrot, Emma Tuypens, Sofie Seghers, Anna Kuchmiy, Elisabeth Brabants, Bart Vanderborght, Ine Maes, Sarah Vandenberghe, Jessica Filtjens, Alícia Gordún Peiró, Daliya Kancheva, Ayla Debraekeleer, Florence Lambolez, Emily De Lombaerde, Yong Chen, Rafael Miyazawa Martins, Pauline Formaglio, Damya Laoui, Sofie Deschoemaeker, Bruno G. De Geest, Rogerio Amino, Stefaan De Koker   +30 more
wiley   +1 more source

From laboratory to market: the biotechnology industry in the Third District [PDF]

In "From Laboratory to Market: The Biotechnology Industry in the Third District," Tim Schiller describes major biotechnology products and reviews estimates of the industry's size and scope. He also outlines where the industry is most active in the United
Timothy Schiller
core  

Microbial Communities of the Providence River [PDF]

, 2015
The Providence River has been industrialized for over one-hundred years. Industries such as oil storage and metal recycling facilities have left high levels of pollutant metals, including lead (Pb), in the soil and water.
Kratch, Jacqueline
core   +1 more source