Results 11 to 20 of about 538,381 (248)
Versatile Epitope Tagging Vector for Gene Expression in Mammalian Cells
BioTechniques, 1998 We have constructed an epitope-tagging vector, pCMV-Tag 1, for gene expression in mammalian cells. This vector, which allows for N-terminal, C-terminal and internal tagging of the gene product of interest with the FLAG® and/or c-myc epitopes, enables ...
Tanya Hosfield, Quinn Lu
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Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning
Frontiers in Bioengineering and Biotechnology, 2020 Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning.
Pu Yan +5 more
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Endogenous leptin promotes autophagy in EBSS-induced PFCs
Animal Cells and Systems, 2019 Leptin is an important adipokine and plays a vital role in animals. However, the role of leptin in the autophagic response of pig fibroblast cells (PFCs) has not been fully elucidated.
Deling Jiao +10 more
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Cloning and characterization of bgl6111 gene encoding β‐glucosidase from bagasse metagenome
Indonesian Journal of Biotechnology, 2023 β‐Glucosidase (BGL) is an essential enzyme for the hydrolysis of cellulose in industrial processes, but natural BGL enzymes are poorly understood. Metagenomics is a robust tool for bioprospecting in the search for novel enzymes from the entire community ...
Fitra Adi Prayogo +5 more
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Cold Spring Harbor Protocols, 2017 Viable and fertile mice can be generated by somatic nuclear transfer into enucleated oocytes, presumably because the transplanted somatic cell genome becomes reprogrammed by factors in the oocyte. The first somatic cloned offspring of mice were obtained by directly injecting donor nuclei into recipient enucleated oocytes.
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Біологія тварин, 2022 The purpose of the work was to analyze the situation regarding to the status and prospects for the use of reproductive biotechnology to increase productivity in cattle breeding.
M. M. Sharan, Yu. T. Salyha
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Cloning Coconut via Somatic Embryogenesis: A Review of the Current Status and Future Prospects
Plants, 2021 Coconut [Cocos nucifera L.] is often called “the tree of life” because of its many uses in the food, beverage, medicinal, and cosmetic industries. Currently, more than 50% of the palms grown throughout the world are senile and need to be replanted ...
Sundaravelpandian Kalaipandian +9 more
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Türk Biyokimya Dergisi, 2023 Voltage-gated calcium channels are essential elements in development of many cellular processes like electrical signaling, contraction secretion and gene expression.
Saglam Berk +5 more
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Polylinguality and Transcultural Practices, 2019 The British-Japanese writer Kazuo Ishiguro was awarded the Nobel Prize for the anti-utopian novel “Do not let me go” in 2017. The authors of the article in the framework of ontological poetics consider the aesthetic advantages of the work, paying close ...
Zukhra A. Kuchukova, Liana B. Berberova
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The First Genomic Analysis of Visna/Maedi Virus Isolates in China
Frontiers in Veterinary Science, 2022 Visna/Maedi virus (VMV) is a neglected pathogen that damages sheep and goats' nervous and respiratory systems. The virus was discovered 80 years ago and has been endemic in China for nearly four decades; nevertheless, there is little information ...
Jian-Yong Wu +6 more
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