Ethanol Metabolism Dynamics in Clostridium ljungdahlii Grown on Carbon Monoxide. [PDF]
Ethanol production from carbon monoxide (CO) as a carbon and energy source by Clostridium ljungdahlii and “ Clostridium autoethanogenum ” is currently being commercialized. During gas fermentation, ethanol synthesis is NADH-dependent.
Liu ZY +7 more
europepmc +4 more sources
Expanding the molecular toolkit for the homoacetogen Clostridium ljungdahlii. [PDF]
AbstractIncreasing interest in homoacetogenic bacteria for the production of biochemicals and biofuels requisites the development of new genetic tools for these atypical production organisms. An attractive host for the conversion of synthesis gas or electricity into multi-carbon compounds is Clostridium ljungdahlii.
Molitor B +4 more
europepmc +8 more sources
Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum. [PDF]
Clostridium autoethanogenum and Clostridium ljungdahlii are physiologically and genetically very similar strict anaerobic acetogens capable of growth on carbon monoxide as sole carbon source. While exact nutritional requirements have not been reported, we observed that for growth, the addition of vitamins to media already containing yeast extract was ...
Annan FJ +8 more
europepmc +4 more sources
Reducing Oxygen Stress and Improving Hydrogen Availability Boosts Microbial Electrosynthesis by Clostridium ljungdahlii. [PDF]
Microbial electrosynthesis (MES) from CO2 by acetogens enables the production of value‐added chemicals. However, its current limitations include O2 stress and insufficient H2 availability. Using Clostridium ljungdahlii as a model in 1‐L electrobioreactors providing high process control, MES is boosted to unprecedent acetate concentrations and rates ...
Kuchenbuch A +6 more
europepmc +2 more sources
Fermentation of biomass-derived syngas to ethanol and acetate by clostridium ljungdahlii [PDF]
In the biochemical pathway of lignocellulosics conversion into fuels, a significant portion of biomass cannot be hydrolysed to fermentable sugars and remains as waste substrate that, due to its recalcitrance, is not converted to ethanol by microorganisms.
Gírio, Francisco +3 more
core +2 more sources
Metabolic response of Clostridium ljungdahlii to oxygen exposure. [PDF]
ABSTRACTClostridium ljungdahliiis an important synthesis gas-fermenting bacterium used in the biofuels industry, and a preliminary investigation showed that it has some tolerance to oxygen when cultured in rich mixotrophic medium. Batch cultures not only continue to grow and consume H2, CO, and fructose after 8% O2exposure, but fermentation product ...
Whitham JM +4 more
europepmc +4 more sources
Lactose-inducible system for metabolic engineering of Clostridium ljungdahlii. [PDF]
ABSTRACT The development of tools for genetic manipulation of Clostridium ljungdahlii has increased its attractiveness as a chassis for autotrophic production of organic commodities and biofuels from syngas and microbial electrosynthesis and established it as a model organism for the study of the basic ...
Banerjee A +4 more
europepmc +4 more sources
Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag and SNAP-tag Proteins. [PDF]
Up to this point, assays and methods involving fluorescent reporter proteins were unavailable or limited in Clostridium organisms and other strict anaerobes. Green fluorescent protein (GFP), mCherry, and flavin-binding proteins (and their derivatives) have been used only in a few clostridia with limited success and ...
Charubin K, Streett H, Papoutsakis ET.
europepmc +4 more sources
Anaerobic Carbon Monoxide Dehydrogenase Diversity in the Homoacetogenic Hindgut Microbial Communities of Lower Termites and the Wood Roach [PDF]
Anaerobic carbon monoxide dehydrogenase (CODH) is a key enzyme in the Wood-Ljungdahl (acetyl-CoA) pathway for acetogenesis performed by homoacetogenic bacteria.
A Brauman +62 more
core +11 more sources
Isobutanol Production by Autotrophic Acetogenic Bacteria
Two different isobutanol synthesis pathways were cloned into and expressed in the two model acetogenic bacteria Acetobacterium woodii and Clostridium ljungdahlii. A.
Sandra Weitz +5 more
doaj +1 more source

