A fluorescence-based assay suitable for quantitative analysis of deadenylase enzyme activity [PDF]
, 2013 In eukaryotic cells, the shortening and removal of the poly(A) tail of cytoplasmic mRNA by deadenylase enzymes is a critical step in posttranscriptional gene regulation.
Fischer, Peter M. +7 more
core +1 more source
Poultry ScienceHeat stress and Salmonella infection are two major challenges threatening poultry health and productivity. Native breeds generally show stronger resistance than commercial lines, but the molecular mechanisms underlying this advantage remain unclear. This
Yaping Wang +7 more
doaj +1 more source
hnRNP C 및 CNOT7 상호 작용을 통한 Bcl-XL mRNA 안정성 하향 조절로 인한 BTG2의 암세포 사멸 촉진 [PDF]
, 2018 BTG2, as a founding member of anti-proliferative gene family, has been reported to render cancer cells more sensitive to chemotherapy and radiotherapy. Recent study suggested that BTG2 can activate general mRNA deadenylation and degradation as a binding ...
최, 용원
core
The anti-proliferative activity of BTG/TOB proteins is mediated via the Caf1a (CNOT7)/Caf1b (CNOT8) deadenylase enzymes [PDF]
The human BTG/TOB protein family comprises six members (BTG1, BTG2/PC3/Tis21, BTG3/Ana, BTG4/PC3B, TOB1/Tob, and TOB2) that display anti-proliferative activity in a number of cell types.
Doidge, Rachel L.
core
Overlap between transcripts identified by RNA immunoprecipitation (RNA-IP) and the microarray analyses (A). [PDF]
, 2016 RNA binding protein motif enrichment for the RIP-seq transcripts (B). Overlap analysis of the CPE, CPSF and NRE RNA binding protein motifs in the 149 transcripts in common between the RIP-seq and microarray experiment (C).
Farhoud Faraji (135917) +6 more
core +1 more source
Analysis of the Ccr4-Not deadenylase complex: a biochemical and computational approach [PDF]
In eukaryotic cells, the degradation of the mRNA poly(A) tail (deadenylation) is a crucial step in the regulation of gene expression. The Ccr4-Not complex is the major deadenylase enzyme involved in the mRNA deadenylation.
Balacco, Dario Leonardo
core +6 more sources
Mtail-Seq For Pan2/3, Caf1A/B (Cnot7/8) Kd Hela Samples (Internal Id: Ms121)
, 2018 mTAIL-seq, MiSeq run (internal ID: ms121) spkN, spk8, spk16, spk32, spk64, spk118, spk128 spike-ins NC2 (siCont), 232 (siPAN2/3), 783 (siCAF1a/b or siCNOT7/8) samples This dataset contains cluster intensities of fluorescence signals that are repacked as an HDF5 formatted file.
Park, Joha, Yi, Hyerim, Kim, V. Narry
openaire +2 more sources
Investigation of the PABPC1-BTG2 interaction and its effects on mRNA deadenylation by CNOT7(CAF1) [PDF]
Polyadenylation is the process of the addition of a poly(A) tail to the 3ʹ end of mRNA. The binding of human poly(A) binding protein to the poly(A) tail can either initiate or repress translation or regulate mRNA degradation. Cytoplasmic mRNA degradation
Ameerul Moominin, Aalam Mohammed
core
Biochemical analysis of the nuclease module of the human Ccr4-Not deadenylase complex [PDF]
, 2015 In eukaryotic cells, the shortening and removal of the poly(A) tail (deadenylation) of cytoplasmic mRNA is a key step in mRNA degradation. The Ccr4-Not complex is well-characterised as a major deadenylase enzyme involved in mRNA deadenylation.
Maryati, Maryati
core
Analysis of the Ccr4-Not deadenylase complex: a biochemical and computational approach [PDF]
, 2017 In eukaryotic cells, the degradation of the mRNA poly(A) tail (deadenylation) is a crucial step in the regulation of gene expression. The Ccr4-Not complex is the major deadenylase enzyme involved in the mRNA deadenylation.
Balacco, Dario Leonardo
core