Cnot8 eliminates naïve regulation networks and is essential for naïve-to-formative pluripotency transition [PDF]
Nucleic Acids Research, 2022 Abstract Mammalian early epiblasts at different phases are characterized by naïve, formative, and primed pluripotency states, involving extensive transcriptome changes. Here, we report that deadenylase Cnot8 of Ccr4-Not complex plays essential roles during the transition from naïve to formative state.
Meijiao Wang +2 more
exaly +4 more sources
Disruption of the Mammalian Ccr4–Not Complex Contributes to Transcription-Mediated Genome Instability [PDF]
Cells, 2023 The mammalian Ccr4–Not complex, carbon catabolite repression 4 (Ccr4)-negative on TATA-less (Not), is a large, highly conserved, multifunctional assembly of proteins that acts at different cellular levels to regulate gene expression.
Morteza Chalabi Hajkarim +2 more
exaly +4 more sources
Importance of CNOT8 Deadenylase Subunit in DNA Damage Responses Following Ionizing Radiation (IR) [PDF]
Reports of Biochemistry and Molecular Biology, 2020 The Ccr4-Not protein complex (CNOT complex) is a key regulator of gene expression in eukaryotic cells. Ccr4-Not Complex is composed of at least nine conserved subunits in mammalian cells with two main enzymatic activities. CNOT8 is a subunit of the complex with deadenylase activity that interacts transiently with the CNOT6 or CNOT6L subunits.
Roger Grand +2 more
exaly +7 more sources
A Mutation in cnot8, Component of the Ccr4-Not Complex Regulating Transcript Stability, Affects Expression Levels of Developmental Regulators and Reveals a Role of Fgf3 in Development of Caudal Hypothalamic Dopaminergic Neurons [PDF]
PLoS ONE, 2014 While regulation of the activity of developmental control genes at the transcriptional level as well as by specific miRNA-based degradation are intensively studied, little is known whether general cellular mechanisms controlling mRNA decay may contribute
Peter Koch, Wolfgang Driever
exaly +6 more sources
Discovery of Substituted 5-(2-Hydroxybenzoyl)-2-Pyridone Analogues as Inhibitors of the Human Caf1/CNOT7 Ribonuclease [PDF]
MoleculesThe Caf1/CNOT7 nuclease is a catalytic component of the Ccr4-Not deadenylase complex, which is a key regulator of post-transcriptional gene regulation. In addition to providing catalytic activity, Caf1/CNOT7 and its paralogue Caf1/CNOT8 also contribute a
Ishwinder Kaur +2 more
exaly +4 more sources
PLoS ONE, 2012 The human BTG/TOB protein family comprises six members (BTG1, BTG2/PC3/Tis21, BTG3/Ana, BTG4/PC3B, TOB1/Tob, and TOB2) that are characterised by a conserved BTG domain.
Rachel Doidge +2 more
exaly +5 more sources
CNOT7 Outcompetes Its Paralog CNOT8 for Integration into The CCR4-NOT Complex
Journal of Molecular Biology, 2022 The CCR4-NOT deadenylase complex is a major post-transcriptional regulator of eukaryotic gene expression. CNOT7 and CNOT8 are both vertebrate homologs of the yeast CCR4-NOT catalytic subunit Caf1. They are highly similar and are sometimes considered redundant, but Cnot7 and Cnot8 knockout mice exhibit different phenotypes, implying distinct ...
Akiko Yanagiya +2 more
exaly +3 more sources
Discovery of Drug-like Inhibitors of the Human Caf1/CNOT7 poly(A)-Selective Nuclease Using Compound Screening [PDF]
BiomoleculesThe human Ccr4–Not complex is a central regulator of post-transcriptional gene regulation, impacting on translation and mRNA degradation. In mRNA degradation, Ccr4–Not participates in the shortening of the mRNA poly(A)-tail via two catalytic subunits ...
Ishwinder Kaur +3 more
doaj +2 more sources
The Ccr4–Not Deadenylase Subunits CNOT7 and CNOT8 Have Overlapping Roles and Modulate Cell Proliferation [PDF]
Molecular Biology of the Cell, 2009 Accurate gene expression requires the precise control of mRNA levels, which are determined by the relative rates of nuclear (pre-)mRNA synthesis and processing, and cytoplasmic mRNA turnover. A key step in mRNA degradation is the removal of the poly(A) tail, which involves several deadenylases including components of the Ccr4–Not complex.
Akhmed Aslam +2 more
exaly +4 more sources
Heterogeneity and complexity within the nuclease module of the Ccr4-Not complex
Frontiers in Genetics, 2013 The shortening of the poly(A) tail of cytoplasmic mRNA (deadenylation) is a pivotal step in the regulation of gene expression in eukaryotic cells. Deadenylation impacts on both regulated mRNA decay as well as the rate of mRNA translation.
G Sebastiaan Winkler, Dario L Balacco
exaly +3 more sources