Results 1 to 10 of about 3,135,932 (255)

The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae

Biology Open, 2016
The rate and regulation of mRNA decay are major elements in the proper control of gene expression. Edc3 and Lsm4 are two decapping activator proteins that have previously been shown to function in the assembly of RNA granules termed P bodies.
Susanne Huch, Maren Müller, Mridula Muppavarapu, Jessie Gommlich, Vidya Balagopal, Tracy Nissan   +5 more
doaj   +1 more source

Long noncoding RNA CASC11 suppresses sorafenib-triggered ferroptosis via stabilizing SLC7A11 mRNA in hepatocellular carcinoma cells

Discover Oncology, 2023
As a frontline treatment for patients with advanced hepatocellular carcinoma (HCC), sorafenib is an effective drug approved by the Food and Drug Administration (FDA).
Fei Chen, Liang Wang
doaj   +1 more source

Kaposi’s Sarcoma-Associated Herpesvirus ORF57 Protein: Exploiting All Stages of Viral mRNA Processing

Viruses, 2013
Nuclear mRNA export is a highly complex and regulated process in cells. Cellular transcripts must undergo successful maturation processes, including splicing, 5'-, and 3'-end processing, which are essential for assembly of an export competent ...
Adrian Whitehouse, Belinda Baquero-Perez, Brian R. Jackson, Sophie Schumann   +3 more
doaj   +1 more source

Concatemeric Broccoli reduces mRNA stability and induces aggregates.

PLoS ONE, 2021
Fluorogenic aptamers are an alternative to established methodology for real-time imaging of RNA transport and dynamics. We developed Broccoli-aptamer concatemers ranging from 4 to 128 substrate-binding site repeats and characterized their behavior fused ...
Marco R Rink, Marisa A P Baptista, Felix J Flomm, Thomas Hennig, Adam W Whisnant, Natalia Wolf, Jürgen Seibel, Lars Dölken, Jens B Bosse   +8 more
doaj   +2 more sources

Pseudomonas mRNA 2.0: Boosting Gene Expression Through Enhanced mRNA Stability and Translational Efficiency

Frontiers in Bioengineering and Biotechnology, 2020
High gene expression of enzymes partaking in recombinant production pathways is a desirable trait among cell factories belonging to all different kingdoms of life.
Dário Neves, Stefan Vos, Lars M. Blank, Birgitta E. Ebert, Birgitta E. Ebert, Birgitta E. Ebert   +5 more
doaj   +1 more source

mRNA Stability Assay Using Transcription Inhibition by Actinomycin D in Mouse Pluripotent Stem Cells

Bio-Protocol, 2018
Gene expression is regulated through multiple steps at both transcriptional and post-transcriptional levels. The net abundance of mature mRNA species in cells is determined by the balance between transcription and degradation.
Madara Ratnadiwakara, Minna-Liisa Änkö
doaj   +1 more source

The Role of lncRNAs in Gene Expression Regulation through mRNA Stabilization

Non-Coding RNA, 2021
mRNA stability influences gene expression and translation in almost all living organisms, and the levels of mRNA molecules in the cell are determined by a balance between production and decay.
Maialen Sebastian-delaCruz, Itziar Gonzalez-Moro, Ane Olazagoitia-Garmendia, Ainara Castellanos-Rubio, Izortze Santin   +4 more
doaj   +1 more source

A comprehensive analysis of the use of nucleoside analogues in RNA therapeutics

Journal of Translational Medicine
Background mRNA therapeutics represent a promising frontier in the development of vaccines, anti-cancer treatments, and gene replacement therapies. The mRNA platform facilitates the rapid design of effective and safe candidates.
Alisa A. Kukushkina-Zvezdova, Anna A. Zherebtsova, Darya V. Telegina, Ivan A. Skvortsov, Roman A. Ivanov, Vasiliy V. Reshetnikov   +5 more
doaj   +1 more source

CleanCap M6 inhibits decapping of exogenously delivered IVT mRNA

Molecular Therapy: Nucleic Acids
Co-transcriptional capping allows exogenous mRNAs to yield robust protein expression. Identifying additional mRNA modifications that further boost protein output will be crucial for developing more efficacious mRNA therapies.
Zachary F. Mandell, Andrew Ujita, Jordana Henderson, Anthony Truong, Coleen Vo, Farinaz Rezvani, Nona Abolhassani, Alexandre Lebedev, Chunping Xu, Inna Koukhareva, Stephanie Ramos, Kate Broderick, Benjamin Hudson, Jeff Coller   +13 more
doaj   +1 more source

Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeutics

Nature Communications, 2022
The authors develop an RNA sequencing-based platform, PERSIST-seq, to simultaneously delineate in-cell mRNA stability, ribosome load, and in-solution stability of a diverse mRNA library to derive design principles for improved mRNA therapeutics.
Kathrin Leppek, Gun Woo Byeon, Wipapat Kladwang, Hannah K. Wayment-Steele, Craig H. Kerr, Adele F. Xu, Do Soon Kim, Ved V. Topkar, Christian Choe, Daphna Rothschild, Gerald C. Tiu, Roger Wellington-Oguri, Kotaro Fujii, Eesha Sharma, Andrew M. Watkins, John J. Nicol, Jonathan Romano, Bojan Tunguz, Fernando Diaz, Hui Cai, Pengbo Guo, Jiewei Wu, Fanyu Meng, Shuai Shi, Eterna Participants, Philip R. Dormitzer, Alicia Solórzano, Maria Barna, Rhiju Das   +28 more
doaj   +1 more source