Results 31 to 40 of about 4,875 (208)

The Role of lncRNAs in Gene Expression Regulation through mRNA Stabilization

Non-Coding RNA, 2021
mRNA stability influences gene expression and translation in almost all living organisms, and the levels of mRNA molecules in the cell are determined by a balance between production and decay.
Maialen Sebastian-delaCruz, Itziar Gonzalez-Moro, Ane Olazagoitia-Garmendia, Ainara Castellanos-Rubio, Izortze Santin   +4 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Matrin 3 Binds and Stabilizes mRNA

PLoS ONE, 2011
Matrin 3 (MATR3) is a highly conserved, inner nuclear matrix protein with two zinc finger domains and two RNA recognition motifs (RRM), whose function is largely unknown. Recently we found MATR3 to be phosphorylated by the protein kinase ATM, which activates the cellular response to double strand breaks in the DNA. Here, we show that MATR3 interacts in
Salton, M., Elkon, R., Borodina, T., Davydov, A., Yaspo, M., Halperin, E., Shiloh, Y.   +6 more
openaire   +5 more sources

Micromanagement: A Role for MicroRNAs in mRNA Stability [PDF]

ACS Chemical Biology, 2006
Small, inhibitory RNA molecules called microRNAs cause large decreases in target protein levels through a post-transcriptional mechanism. Until recently, it was believed this mechanism operated almost exclusively at a step in translation. However, new work has revealed that microRNAs have a second, post-transcriptional mechanism that accelerates the ...
Sarah F, Roush, Frank J, Slack
openaire   +2 more sources

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

A comprehensive analysis of the use of nucleoside analogues in RNA therapeutics

Journal of Translational Medicine
Background mRNA therapeutics represent a promising frontier in the development of vaccines, anti-cancer treatments, and gene replacement therapies. The mRNA platform facilitates the rapid design of effective and safe candidates.
Alisa A. Kukushkina-Zvezdova, Anna A. Zherebtsova, Darya V. Telegina, Ivan A. Skvortsov, Roman A. Ivanov, Vasiliy V. Reshetnikov   +5 more
doaj   +1 more source

CleanCap M6 inhibits decapping of exogenously delivered IVT mRNA

Molecular Therapy: Nucleic Acids
Co-transcriptional capping allows exogenous mRNAs to yield robust protein expression. Identifying additional mRNA modifications that further boost protein output will be crucial for developing more efficacious mRNA therapies.
Zachary F. Mandell, Andrew Ujita, Jordana Henderson, Anthony Truong, Coleen Vo, Farinaz Rezvani, Nona Abolhassani, Alexandre Lebedev, Chunping Xu, Inna Koukhareva, Stephanie Ramos, Kate Broderick, Benjamin Hudson, Jeff Coller   +13 more
doaj   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeutics

Nature Communications, 2022
The authors develop an RNA sequencing-based platform, PERSIST-seq, to simultaneously delineate in-cell mRNA stability, ribosome load, and in-solution stability of a diverse mRNA library to derive design principles for improved mRNA therapeutics.
Kathrin Leppek, Gun Woo Byeon, Wipapat Kladwang, Hannah K. Wayment-Steele, Craig H. Kerr, Adele F. Xu, Do Soon Kim, Ved V. Topkar, Christian Choe, Daphna Rothschild, Gerald C. Tiu, Roger Wellington-Oguri, Kotaro Fujii, Eesha Sharma, Andrew M. Watkins, John J. Nicol, Jonathan Romano, Bojan Tunguz, Fernando Diaz, Hui Cai, Pengbo Guo, Jiewei Wu, Fanyu Meng, Shuai Shi, Eterna Participants, Philip R. Dormitzer, Alicia Solórzano, Maria Barna, Rhiju Das   +28 more
doaj   +1 more source