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RNA Stability Measurements Using RT-qPCR in Arabidopsis Seedlings
Bio-Protocol, 2020 Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions.
Tianran Jia, Brandon Le
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Control of mRNA Stability by SAPKs [PDF]
, 2007 Control of mRNA turnover is an essential step in the regulation of gene expression in eukaryotes. The concerted action of many enzymes regulates the way each mRNA is degraded. Moreover, the degradation of each mRNA is influenced by the environment surrounding the cell.
Miguel A, Rodríguez-Gabriel +1 more
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Biology Open, 2016 The rate and regulation of mRNA decay are major elements in the proper control of gene expression. Edc3 and Lsm4 are two decapping activator proteins that have previously been shown to function in the assembly of RNA granules termed P bodies.
Susanne Huch +5 more
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Viruses, 2013 Nuclear mRNA export is a highly complex and regulated process in cells. Cellular transcripts must undergo successful maturation processes, including splicing, 5'-, and 3'-end processing, which are essential for assembly of an export competent ...
Adrian Whitehouse +3 more
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Cellular polyamines modulate mRNA stability [PDF]
Plant Signaling & Behavior, 2017 Polyamines (PAs) are polycationic compounds found in all living organisms and play crucial roles in growth and survival. PAs interact with and modulate the functions of anionic macromolecules such as DNA, RNA and proteins. LHR1/PUT3 is a polyamine influx transporter localized in the plasma membrane in Arabidopsis.
Haoxi Chai, Wannian Yang, Huazhong Shi
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Concatemeric Broccoli reduces mRNA stability and induces aggregates
PLoS ONE, 2021 Fluorogenic aptamers are an alternative to established methodology for real-time imaging of RNA transport and dynamics. We developed Broccoli-aptamer concatemers ranging from 4 to 128 substrate-binding site repeats and characterized their behavior fused ...
Marco R. Rink +8 more
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Frontiers in Bioengineering and Biotechnology, 2020 High gene expression of enzymes partaking in recombinant production pathways is a desirable trait among cell factories belonging to all different kingdoms of life.
Dário Neves +5 more
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Discover Oncology, 2023 As a frontline treatment for patients with advanced hepatocellular carcinoma (HCC), sorafenib is an effective drug approved by the Food and Drug Administration (FDA).
Fei Chen, Liang Wang
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mRNA Stability Assay Using Transcription Inhibition by Actinomycin D in Mouse Pluripotent Stem Cells
Bio-Protocol, 2018 Gene expression is regulated through multiple steps at both transcriptional and post-transcriptional levels. The net abundance of mature mRNA species in cells is determined by the balance between transcription and degradation.
Madara Ratnadiwakara, Minna-Liisa Änkö
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Regulation of mRNA stability in mammalian cells [PDF]
Gene, 2001 The regulation of mRNA decay is a major control point in gene expression. The stability of a particular mRNA is controlled by specific interactions between its structural elements and RNA-binding proteins that can be general or mRNA-specific. Regulated mRNA stability is achieved through fluctuations in half-lives in response to developmental or ...
J, Guhaniyogi, G, Brewer
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