Coelenterazine Analogs for Bioassays and Molecular Imaging [PDF]
SensorsCoelenterazine (CTZ) is a common substrate of marine luciferases upon emission of bioluminescence (BL) in living organisms. Because CTZ works as a “luminophore” in the process of BL emission, the chemical modification has been centered for improving the ...
Sung-Bae Kim +3 more
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Coelenterazine sulfotransferase from Renilla muelleri.
PLoS ONE, 2022 The luciferin sulfokinase (coelenterazine sulfotransferase) of Renilla was previously reported to activate the storage form, luciferyl sulfate (coelenterazine sulfate) to luciferin (coelenterazine), the substrate for the luciferase bioluminescence ...
George Tzertzinis +7 more
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Fast kinetics of photoprotein emitting species [PDF]
Scientific ReportsCoelenterazine is the most common substrate for light-emitting reactions identified in luminous marine organisms. Among bioluminescent proteins engaging coelenterazine as a luciferin, Ca2+-regulated photoproteins form stable enzyme-substrate complexes ...
Elena V. Eremeeva +4 more
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Availability and occurrence of coelenterazine in a Swedish fjord to maintain Amphiura filiformis bioluminescence [PDF]
Scientific ReportsThe bioluminescent European brittle star Amphiura filiformis produces blue light at the arm-spine level thanks to a biochemical reaction involving coelenterazine as substrate and a Renilla-like luciferase as an enzyme.
Constance Coubris +3 more
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Insights into the bioluminescence systems of three sea pens (Cnidaria: Anthozoa): from de novo transcriptome analyses to biochemical assays [PDF]
Open BiologyBioluminescence is the production of visible light by living organisms. It occurs through the oxidation of luciferin substrates catalysed by luciferase enzymes.
Laurent Duchatelet +10 more
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Design of Ctenophore Ca2+-Regulated Photoprotein Berovin Capable of Being Converted into Active Protein Under Physiological Conditions: Computational and Experimental Approaches [PDF]
LifeHere, we describe (1) the AlphaFold-based structural modeling approach to identify amino acids of the photoprotein berovin that are crucial for coelenterazine binding, and (2) the production and characterization of berovin mutants with substitutions of ...
Ludmila P. Burakova +4 more
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Advanced Bioluminescence Reporter with Engineered Gaussia Luciferase via Sequence-Guided Mutagenesis [PDF]
BiosensorsGaussia luciferase (GLuc) is the preeminent secreted luciferase widely used in cell-based reporter assays. By employing sequence-guided mutagenesis informed by alignments of diverse copepod luciferase sequences, we identified key amino acids that ...
Vinayakumar Gedi +3 more
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The key residue responsible for the red shift of bioluminescence spectra of light-sensitive Ca2+-regulated photoproteins of ctenophores [PDF]
Scientific ReportsIsoforms of some ctenophore photoproteins show different maxima of bioluminescence spectra— the ones of mnemiopsin and bolinopsin have λmax at 490 and 500 nm, while λmax for velamin isoforms are at 500 and 508 nm.
Ludmila P. Burakova, Eugene S. Vysotski
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Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays [PDF]
Scientific Reports, 2017 Spectral overlaps among the multiple optical readouts commonly cause optical contamination in fluorescence and bioluminescence. To tackle this issue, we created five-different lineages of coelenterazine (CTZ) analogues designed to selectively illuminate ...
Ryo Nishihara +5 more
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Mutational optimization of the coelenterazine-dependent luciferase from
Plant Methods, 2008 Renilla luciferase (RLUC) is a popular reporter enzyme for gene expression and biosensor applications, but it is an unstable enzyme whose catalytic mechanism remains to be elucidated.
Woo Jongchan, von Arnim Albrecht G
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