Results 21 to 30 of about 5,587 (151)

Localization of the Catalytic Domain of Copepod Luciferases: Analysis of Truncated Mutants of the Metridia longa Luciferase

Life, 2023
Luciferases from copepods Metridia longa and Gaussia princeps are successfully used as bioluminescent reporters for in vivo and in vitro assays. Here, we report the minimal sequence of copepod luciferases required for bioluminescence activity that was ...
Svetlana V. Markova, Marina D. Larionova, Igor A. Korotov, Eugene S. Vysotski   +3 more
doaj   +1 more source

Creation of Artificial Luciferase 60s from Sequential Insights and Their Applications to Bioassays

Sensors, 2023
In this study, a series of new artificial luciferases (ALucs) was created using sequential insights on missing peptide blocks, which were revealed using the alignment of existing ALuc sequences.
Sung-Bae Kim, Tadaomi Furuta, Nobuo Kitada, Shojiro A. Maki   +3 more
doaj   +1 more source

Target-Oriented Synthesis of Marine Coelenterazine Derivatives with Anticancer Activity by Applying the Heavy-Atom Effect

Biomedicines, 2021
Photodynamic therapy (PDT) is an anticancer therapeutic modality with remarkable advantages over more conventional approaches. However, PDT is greatly limited by its dependence on external light sources.
Carla M. Magalhães, Patricia González-Berdullas, Diana Duarte, Ana Salomé Correia, José E. Rodríguez-Borges, Nuno Vale, Joaquim C. G. Esteves da Silva, Luís Pinto da Silva   +7 more
doaj   +1 more source

Cardioluminescence in Transgenic Zebrafish Larvae: A Calcium Imaging Tool to Study Drug Effects and Pathological Modeling

Biomedicines, 2021
Zebrafish embryos and larvae have emerged as an excellent model in cardiovascular research and are amenable to live imaging with genetically encoded biosensors to study cardiac cell behaviours, including calcium dynamics. To monitor calcium ion levels in
Manuel Vicente, Jussep Salgado-Almario, Michelle M. Collins, Antonio Martínez-Sielva, Masafumi Minoshima, Kazuya Kikuchi, Beatriz Domingo, Juan Llopis   +7 more
doaj   +1 more source

Reverse mutants of the catalytic 19 kDa mutant protein (nanoKAZ/nanoLuc) from Oplophorus luciferase with coelenterazine as preferred substrate.

PLoS ONE, 2022
Native Oplophorus luciferase (OpLase) and its catalytic 19 kDa protein (wild KAZ) show highest luminescence activity with coelenterazine (CTZ) among CTZ analogs.
Satoshi Inouye, Jun-Ichi Sato, Yuiko Sahara-Miura, Yuri Tomabechi, Yuto Sumida, Shun-Ichi Sekine, Mikako Shirouzu, Takamitsu Hosoya   +7 more
doaj   +2 more sources

Red-Shifted Aequorin Variants Incorporating Non-Canonical Amino Acids: Applications in In Vivo Imaging. [PDF]

PLoS ONE, 2016
The increased importance of in vivo diagnostics has posed new demands for imaging technologies. In that regard, there is a need for imaging molecules capable of expanding the applications of current state-of-the-art imaging in vivo diagnostics.
Kristen M Grinstead, Laura Rowe, Charles M Ensor, Smita Joel, Pirouz Daftarian, Emre Dikici, Jean-Marc Zingg, Sylvia Daunert   +7 more
doaj   +1 more source

Biophysical analysis of Gaussia luciferase bioluminescence mechanisms using a non-oxidizable coelenterazine

BBA Advances, 2023
Gaussia luciferase (GLuc 18.2kDa; 168 residues) is a marine copepod luciferase that emits a bright blue light when oxidizing coelenterazine (CTZ). It is a helical protein where two homologous sequential repeats form two anti-parallel bundles, each made ...
Kyoko Takatsu, Naohiro Kobayashi, Nan Wu, Yves L. Janin, Toshio Yamazaki, Yutaka Kuroda   +5 more
doaj   +1 more source

Luciferin production and luciferase transcription in the bioluminescent copepod Metridia lucens [PDF]

PeerJ, 2018
Bioluminescent copepods are often the most abundant marine zooplankton and play critical roles in oceanic food webs. Metridia copepods exhibit particularly bright bioluminescence, and the molecular basis of their light production has just recently begun ...
Michael Tessler, Jean P. Gaffney, Jason M. Crawford, Eric Trautman, Nehaben A. Gujarati, Philip Alatalo, Vincent A. Pieribone, David F. Gruber   +7 more
doaj   +2 more sources

Chemiluminescence Imaging of Superoxide Anion Detects Beta-Cell Function and Mass. [PDF]

PLoS ONE, 2016
Superoxide anion is produced during normal cellular respiration and plays key roles in cellular physiology with its dysregulation being associated with a variety of diseases.
Laura L Bronsart, Christian Stokes, Christopher H Contag   +2 more
doaj   +1 more source

Combined Experimental and Theoretical Investigation into the Photophysical Properties of Halogenated Coelenteramide Analogs

Molecules, 2022
Marine Coelenterazine is one of the most well-known chemi-/bioluminescent systems, and in which reaction the chemi-/bioluminophore (Coelenteramide) is generated and chemiexcited to singlet excited states (leading to light emission).
Ana Carolina P. Afonso, Patricia González-Berdullas, Joaquim C. G. Esteves da Silva, Luís Pinto da Silva   +3 more
doaj   +1 more source