Results 71 to 80 of about 5,587 (151)

Add Coelenterazine-h v1

AddCoelenterazine-has the substrate (light-sensitive; prepare fresh on the day of use) to activate the full-length RenG luciferase and measure its maximum luminescence value. This serves as the relative standard for subsequent SLCA interaction signals.
openaire   +1 more source

Tumor-targeting bioluminescent bacteria for in vivo imaging

Engineering Microbiology
As the understanding of bacteria-mediated cancer therapies deepens, bacteria such as Escherichia coli Nissle 1917 (EcN) have become a promising platform for cancer therapy.
Chenghao Ma, Jingxi Liu, Hongjing Liu, Xiaohan Zhao, Geng Li, Youming Zhang, Tianyu Jiang   +6 more
doaj   +1 more source

Optimized in vivo multispectral bioluminescent imaging of tumor biology using engineered BRET reporters

iScience
Summary: The ability to visualize and track multiple biological processes in vivo in real time is highly desirable. Bioluminescence imaging (BLI) has emerged as an attractive modality for non-invasive cell tracking, with various luciferase reporters ...
Bryan Labra, Kshitij Parag-Sharma, John J. Powers, Sonal Srivastava, Joel R. Walker, Thomas A. Kirkland, Caroline K. Brennan, Jennifer A. Prescher, Antonio L. Amelio   +8 more
doaj   +1 more source

BODIPY-coelenterazine conjugates as self-illuminating substrates for NanoLuc

Chemical Communications
Excitation-free probes can exploit the spectroscopic properties of BODIPYs via bioluminescence resonance energy transference (BRET) triggered by luciferase-like enzymes.
Valeska Viereckt, Frank Abendroth, Alexander Schauerte, Marina Gerhard, Crispin Lichtenberg, Dmitri Kosenkov, Olalla Vázquez   +6 more
openaire   +2 more sources

A Comprehensive Exploration of Bioluminescence Systems, Mechanisms, and Advanced Assays for Versatile Applications

Biochemistry Research International
Bioluminescence has been a fascinating natural phenomenon of light emission from living creatures. It happens when the enzyme luciferase facilitates the oxidation of luciferin, resulting in the creation of an excited-state species that emits light ...
Asiri N. Dunuweera, Shashiprabha P. Dunuweera, K. Ranganathan   +2 more
doaj   +1 more source

The role of His 16, Tyr 82 and Trp 86 in color shift, activity and thermostability of aequorin photoprotein

Iranian South Medical Journal, 2015
Background: Aequorin is a calcium sensitive photoprotein composed of apoaequorin (189 amino acid), oxygen and coelenterazine (imidazopyrazine chromophore). Activity of this photoprotein in calcium presence is blue photon emission (469 nm).
Faeazeh Ghanbartalab, Mehdi Zeinoddini, Saman Hosseinkhani, Masoome Esmailinejad, sayedealah Hosseini, Masoome Laali   +5 more
doaj  

C-series coelenterazine-driven bioluminescence signature imaging

C-series coelenterazine-driven bioluminescence signature imaging
identifier:oai:t2r2.star.titech.ac.jp ...
openaire   +1 more source

Coelenterazine alleviates sepsis-associated acute kidney injury by inhibiting ferroptosis via the USP14-NCOA4 pathway. [PDF]

Transl Androl Urol
Bao D, Sha X, Zhao C, Xie X, Lai Y, Liu L, Fan R, Huang W, Zhang Y, Wu G, Ye D, Li H, Lai C.   +12 more
europepmc   +1 more source

Quantitative assessment of porin-mediated solute transport in biomimetic membranes. [PDF]

Appl Microbiol Biotechnol
Scherer M, Tom Dieck T, Pourtalebi Jahromi L, Schober R, Schäfer M, Castiglione K.   +5 more
europepmc   +1 more source

Applications of bioluminescence-mediated photodynamic therapy (BL-PDT) for the treatment of deep-seated tumours. [PDF]

Discov Oncol
Mohlongo M, George BP, Abrahamse H.
europepmc   +1 more source