Results 51 to 60 of about 251,552 (274)

The molecular basis of the effect of temperature on enzyme activity

, 2010
Experimental data show that the effect of temperature on enzymes cannot be adequately explained in terms of a two-state model based on increases in activity and denaturation.
Oudshoorn, Matthew Leslie, Kelly, S.M., Price, N.C., Weinberg, C.S., Monk, C.R., Oudshoorn, M.L., Monk, Colin R., Danson, Michael J., Price, Nicholas C., Danson, M.J., Lee, Charles Kai-Wu, Peterson, Michelle E., Lee, C.K., Peterson, M.E., Weinberg, Cristina S., Daniel, R.M., Daniel, Roy M., Kelly, Sharon M.   +17 more
core   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

Molecular insights into cold active polygalacturonase enzyme for its potential application in food processing [PDF]

Journal of Food Science and Technology, 2014
Pectin is a complex structural heteropolysaccharide that require numerous pectinolytic enzymes for its complete degradation. Polygalacturonase from mesophilic or thermophilic origin are being widely used in fruit and vegetable processing in the recent decades to degrade pectic substances.
L N, Ramya, K K, Pulicherla
openaire   +2 more sources

pH‐mediated activation of the lysosomal arginine sensor SLC38A9

FEBS Letters, EarlyView.
Cells monitor nutrient levels via the lysosomal transporter SLC38A9 to activate the mechanistic target of rapamycin complex 1 (mTORC1). This study reveals that SLC38A9 function is regulated by pH. We identified histidine 544 as a critical pH sensor that undergoes conformational changes to control amino acid efflux from lysosomes; therefore, it ...
Xuelang Mu, Ampon Sae Her, Tamir Gonen
wiley   +1 more source

Quantitative comparison of catalytic mechanisms and overall reactions in convergently evolved enzymes : implications for classification of enzyme function [PDF]

, 2010
The authors thank the National Institutes of Health (NIH R01 GM60595 to PCB) and the Scottish Universities Life Sciences Alliance (SULSA to JBOM) for funding.Functionally analogous enzymes are those that catalyze similar reactions on similar substrates ...
Almonacid, Daniel E, Daniel E Almonacid, Yera, Emmanuel R., Yera, Emmanuel R, Babbitt, Patricia C., Babbitt, Patricia C, John B O Mitchell, Almonacid Daniel E., Almonacid, Daniel E., Patricia C Babbitt, Yera Emmanuel R., Mitchell, John BO, Mitchell John B. O., Babbitt Patricia C., Emmanuel R Yera, Mitchell, John B. O.   +15 more
core   +1 more source

Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues

, 2022
Cold environments characterised by diverse temperatures close to or below the water freezing point dominate about 80% of the Earth’s biosphere. One of the survival strategies adopted by microorganisms living in cold environments is their expression of ...
Adam Thean Chor Leow, Ahmad Kamarudin, Nor Hafizah, Leow, Adam Thean Chor, Adamu Idris Matinja, Ali, Mohd Shukuri Mohamad, Siti Nurbaya Oslan, Matinja, Adamu Idris, Kamarudin, Nor Hafizah Ahmad, Mohd Shukuri Mohamad Ali, Thean, Adam Chor Leow, Nor Hafizah Ahmad Kamarudin, Mohamad Ali, Mohd Shukuri, Oslan, Siti Nurbaya   +12 more
core   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

Cold-active enzymes in food biotechnology: An updated mini review [PDF]

Journal of Applied Biology & Biotechnology, 2018
Cold-active enzymes and their anticipated application in various industries including food industry attracted attention of worldwide scientific community. Cold-active enzymes, also known as psychrophilic enzymes, possess high catalytic activity at low and moderate temperatures.
openaire   +1 more source

Protein purification and gene isolation of chlamysin, a cold‐active lysozyme‐like enzyme with antibacterial activity [PDF]

FEBS Letters, 1999
An antibacterial ∼11 kDa protein designated chlamysin was isolated from viscera of the marine bivalve Chlamys islandica. Chlamysin inhibited the growth of all Gram‐positive and Gram‐negative bacteria tested. The isolated protein was highly efficient in hydrolyzing Micrococcus luteus cells only at low pH (4.5–6.2) and at low temperature (4–35°C).
Nilsen, Inge W., Øverbø, Kersti, Sandsdalen, Erling, Sandaker, Elin, Sletten, Knut, Myrnes, Bjørnar   +5 more
openaire   +2 more sources

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source