Results 71 to 80 of about 5,105,198 (264)

Improving the Stability of Cold-Adapted Enzymes by Immobilization

, 2017
Cold-adapted enzymes have gained considerable attention as biocatalysts that show high catalytic activity at low temperatures. However, the use of cold-adapted enzymes at ambient temperatures has been hindered by their low thermal stabilities caused by ...
ChangWoo Lee, Sei-Heon Jang, Hye-Shin Chung   +2 more
core   +1 more source

Effects of chronic cold treatment on root elongation and gene expression in "Arabidopsis thaliana" [PDF]

, 2008
Low temperature is a major limitation of plant growth. Cold adaptation is important for the survival and distribution of plant species at high elevations and high latitudes.
Lee, Yang Ping
core   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source

Isolation and identification of a strain producing cold-adapted β galactosidase, and purification and characterisation of the enzyme

Czech Journal of Food Sciences, 2008
Enzymes with high specific activities at low temperatures have potential uses in the food industry. Cold-adapted microorganisms are potentially useful sources of cold-active enzyme.
Wen-Yu Liu, Ying-Wu Shi, Xin-Qin Wang, Yun Wang, Chang-Qing Wei, Kai Lou   +5 more
doaj   +1 more source

KDAC6 alters cell morphology and motility through positive and negative modulation of F‐actin distribution

FEBS Open Bio, EarlyView.
KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph, Andrea B. Pham, Kiara E. Bornes, Razan H. Hammad, Chelsea A. Rousseve‐Ross, Thomas M. Huckaba, Terry J. Watt, Tasha B. Toro   +7 more
wiley   +1 more source

Optimizing and purifying extracellular cold- active lipase from Talaromyces pinophilus AUMC 308 for potential use in industrial detergents [PDF]

Assiut University Journal of Multidisciplinary Scientific Research
In this study, a strain of Talaromyces pinophilus AUMC 308 was used. The 15th days of fermentation at pH 6.0, 15°C with ammonium chloride as the nitrogen source were shown to be the optimal settings for the synthesis of the enzyme.
Asmaa M.A. Omar, Abdel-Nasser A. Zohri, Ahmed Y. Abdel-Malek, Osama Abdel-Hafeez Mohamed Al-Bedak   +3 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Cold-adapted microorganisms for climate-smart agriculture: Diversity, adaptation, and biotechnological applications

Climate Smart Agriculture
Climate change has intensified the frequency and severity of low-temperature stress across many agricultural regions worldwide, posing a major threat to agricultural productivity, ecosystem stability, and global food security. Cold-adapted microorganisms,
Rajeshwari Negi, Tawseefa Jan, Babita Sharma, Preety Tomar, Samrendra Singh Thakur, Karthik S. Narayan, Naseer Ahmed, Mukul Machhindra Barwant, Sangram Singh, Rashmi Mishra, Neelam Yadav, Ajar Nath Yadav   +11 more
doaj   +1 more source

Characterization of a cold-active, detergent-stable metallopeptidase purified from Bacillus sp. S1DI 10 using Response Surface Methodology.

PLoS ONE, 2019
The colder regions of Earth are inhabited by cold-adapted microorganisms designated as psychrophiles that are known to produce cold-active enzymes, such as peptidases, chaperones, lipases, cellulases, and phosphatases.
Drishtant Singh, Sharad Thakur, Seema Madhumal Thayil, Anup Kumar Kesavan   +3 more
doaj   +1 more source

Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP‐ribosylating toxin AB to establish a persistent infection

FEBS Open Bio, EarlyView.
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez, Juliette K. Tinker, Teresa Frisan, Taj Azarian, Ken Teter   +4 more
wiley   +1 more source