Results 11 to 20 of about 1,931,388 (325)
Bio-Protocol, 2013 The Comet assay (or Single Cell Gel Electrophoresis assay) is a sensitive technique to detect DNA damage at the level of an individual cell. This technique is based on micro-electrophoresis of cells DNA content.
Elisa Boutet-Robinet +2 more
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Controlling variation in the comet assay [PDF]
Frontiers in Genetics, 2014 Variability of the comet assay is a serious issue, whether it occurs from experiment to experiment in the same laboratory, or between different laboratories analysing identical samples.
Andrew Richard Collins +5 more
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The Comet Assay: Tails of the (Un)expected. Use of the comet assay in pharmaceutical development.
Frontiers in Genetics, 2015 In genotoxicity testing of pharmaceuticals the rodent alkaline comet assay is being increasingly used as a second in vivo assay in addition to the in vivo micronucleus assay to mitigate in vitro positive results as recommended by regulatory guidance.
Bas-jan Van Der Leede
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Comet assay in myelodysplastic syndromes [PDF]
Revista Brasileira de Hematologia e Hemoterapia, 2012 The comet assay (single-cell gel electrophoresis) has been established as a simple, rapid, flexible and sensitive method of detecting DNA damage in single cells(1,2). Cells embedded in agarose on a microscope slide are lysed with detergent. Electrophoresis at high pH results in structures resembling comets, observed by fluorescence microscopy; the ...
Paulo Florentino Teixeira Neto +2 more
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The next three decades of the comet assay: a report of the 11th International Comet Assay Workshop. [PDF]
Mutagenesis, 2017 The International Comet Assay Workshops are a series of scientific conferences dealing with practical and theoretical aspects of the Comet Assay (single-cell gel electrophoresis)-a simple method for detecting DNA strand breaks. The first paper describing
G. Koppen +5 more
semanticscholar +3 more sources
DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death
Scientific Reports, 2021 The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations.
S. Bonassi +62 more
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Archives of Toxicology, 2021 The comet assay is a commonly used method to determine DNA damage and repair activity in many types of samples. In recent years, the use of the comet assay in human biomonitoring became highly attractive due to its various modified versions, which may be
E. Bankoglu +6 more
semanticscholar +1 more source
Neutral Comet Assay to Detect and Quantitate DNA Double-Strand Breaks in Hematopoietic Stem Cells
Bio-Protocol, 2021 In vertebrates, hematopoietic stem cells (HSCs) regulate the supply of blood cells throughout the lifetime and help to maintain homeostasis. Due to their long lifespan, genetic integrity is paramount for these cells, and accordingly, a number of stem ...
Irene Roy, Pon Nadar, Satish Khurana
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New Application of the Comet Assay [PDF]
Journal of Histochemistry & Cytochemistry, 2011 The comet assay is a well-established, simple, versatile, visual, rapid, and sensitive tool used extensively to assess DNA damage and DNA repair quantitatively and qualitatively in single cells. The comet assay is most frequently used to analyze white blood cells or lymphocytes in human biomonitoring studies, although other cell types have been ...
Elva I. Cortés-Gutiérrez +10 more
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Statistical analysis of comet assay results [PDF]
Frontiers in Genetics, 2014 The comet assay can distinguish small differences in DNA damage between different samples of cells, implying that statistical tests are important to assess whether this occurs by chance. Excellent scholarly papers with concise descriptions of statistical analysis and recommendations for tests have been published (Lovell et al., 1999; Lovell and Omori ...
Peter eMoller, Steffen eLoft
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