Results 11 to 20 of about 1,981,264 (328)

Neutral Comet Assay

Bio-Protocol, 2013
The Comet assay (or Single Cell Gel Electrophoresis assay) is a sensitive technique to detect DNA damage at the level of an individual cell. This technique is based on micro-electrophoresis of cells DNA content.
Elisa Boutet-Robinet, Didier Trouche, Yvan Canitrot   +2 more
doaj   +6 more sources

The use of DNA repair inhibitors and the comet assay-an overview. [PDF]

Mutagenesis
Saenz-Martinez E, López de Cerain A, Azqueta A.   +2 more
europepmc   +2 more sources

DNA damage in canine leishmaniasis infection is detectable by micronucleus and comet assay in peripheral blood samples. [PDF]

Braz J Vet Med
Ruiz RT, Batista ACA, Maria Ruiz JL.
europepmc   +3 more sources

Genotoxicity Evaluation of Metformin in Freshwater Planarian Dugesia japonica by the Comet Assay and RAPD Analysis. [PDF]

Biomed Res Int, 2022
Yin D, Xu Z, Feng M, Zhao Z, Chen D, Song L.   +5 more
europepmc   +3 more sources

B-Comet Assay (Comet Assay on Buccal Cells) for the Evaluation of Primary DNA Damage in Human Biomonitoring Studies. [PDF]

Int J Environ Res Public Health, 2020
Russo C, Acito M, Fatigoni C, Villarini M, Moretti M.   +4 more
europepmc   +3 more sources

Comet assay in myelodysplastic syndromes [PDF]

Revista Brasileira de Hematologia e Hemoterapia, 2012
The comet assay (single-cell gel electrophoresis) has been established as a simple, rapid, flexible and sensitive method of detecting DNA damage in single cells(1,2). Cells embedded in agarose on a microscope slide are lysed with detergent. Electrophoresis at high pH results in structures resembling comets, observed by fluorescence microscopy; the ...
Paulo Florentino Teixeira Neto, Ronald Feitosa Pinheiro, Romélia Pinheiro Gonçalves   +2 more
doaj   +5 more sources

Comet assay: a versatile but complex tool in genotoxicity testing.

Toxicological Research, 2021
The comet assay is a versatile method for measuring DNA strand breaks in individual cells. It can also be applied to cells isolated from treated animals.
E. Cordelli, M. Bignami, F. Pacchierotti
semanticscholar   +1 more source

GamaComet: A Deep Learning-Based Tool for the Detection and Classification of DNA Damage from Buccal Mucosa Comet Assay Images

Diagnostics, 2022
Comet assay is a simple and precise method to analyze DNA damage. Nowadays, many research studies have demonstrated the effectiveness of buccal mucosa cells usage in comet assays.
Afiahayati, Edgar Anarossi, Ryna Dwi Yanuaryska, Sri Mulyana   +3 more
doaj   +1 more source

DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

Scientific Reports, 2021
The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations.
S. Bonassi, M. Ceppi, P. Møller, A. Azqueta, M. Milić, M. Neri, G. Brunborg, R. Godschalk, G. Koppen, S. Langie, J. Teixeira, M. Bruzzone, Juliana da Silva, D. Benedetti, D. Cavallo, C. L. Ursini, L. Giovannelli, S. Moretti, P. Riso, Cristian Del Bo’, P. Russo, M. Dobrzyńska, I. Goroshinskaya, E. Surikova, M. Staruchová, M. Barančoková, K. Volkovová, A. Kažimı́rová, B. Smolková, B. Laffon, V. Valdiglesias, S. Pastor, R. Marcos, Alba Hernández, G. Gajski, B. Spremo-Potparević, L. Živković, É. Boutet-Robinet, H. Perdry, P. Lebailly, C. Pérez, N. Başaran, Zsuzsanna Nemeth, A. Sáfár, M. Dusinska, A. Collins, Diana Vanessa Cristiana Costa Solange Kristine B. Carina Anderson Andrade Pereira Costa Gutzkow Ladeira Mor, Diana Anderson, Vanessa Andrade, C. Pereira, S. Costa, K. Gutzkow, C. Ladeira, M. Moretti, C. Costa, I. Orlow, E. Rojas, B. Pourrut, M. Kruszewski, S. Knasmueller, Sergey Shaposhnikov, B. Žegura, H. Stopper   +62 more
semanticscholar   +1 more source

Neutral Comet Assay to Detect and Quantitate DNA Double-Strand Breaks in Hematopoietic Stem Cells

Bio-Protocol, 2021
In vertebrates, hematopoietic stem cells (HSCs) regulate the supply of blood cells throughout the lifetime and help to maintain homeostasis. Due to their long lifespan, genetic integrity is paramount for these cells, and accordingly, a number of stem ...
Irene Roy, Pon Nadar, Satish Khurana
doaj   +1 more source