Single Cell Analysis of Human RAD18-Dependent DNA Post-Replication Repair by Alkaline Bromodeoxyuridine Comet Assay [PDF]
, 2013 Damage to DNA can block replication progression resulting in gaps in the newly synthesized DNA. Cells utilize a number of post-replication repair (PRR) mechanisms such as the RAD18 controlled translesion synthesis or template switching to overcome the ...
Downes, C. Stephen +4 more
core +5 more sources
An integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses [PDF]
, 2014 The application of the Comet assay in environmental monitoring remains challenging in face of the complexity of environmental stressors, e.g., when dealing with estuarine sediments, that hampers the drawing of cause-effect relationships.
Caeiro, Sandra +8 more
core +1 more source
Stochastic modeling for the COMET-assay [PDF]
, 2003 We present a stochastic model for single cell gel electrophoresis (COMET-assay) data. Essential is the use of point process structures, renewal theory and reduction to intensity histograms for further data ...
Boulesteix, Anne-Laure +2 more
core +3 more sources
Using a medium-throughput comet assay to evaluate the global DNA methylation status of single cells
Frontiers in Genetics, 2014 The comet assay is a simple and cost effective technique, commonly used to analyse and quantify DNA damage in individual cells. The versatility of the comet assay allows introduction of various modifications to the basic technique.
Angelique eLewies +5 more
doaj +1 more source
Genotoxicity of nitroso compounds and sodium dichromate in a model combining organ cultures of human nasal epithelia and the comet assay [PDF]
, 2001 Genotoxic effects of xenobiotics are a possible step in tumor initiation in the mucosa of the upper aerodigestive tract. Using the comet assay, detecting genotoxicity in human tissue has been restricted to single incubations in vitro, but in vivo most ...
Bergner, Albrecht +7 more
core +1 more source
Frontiers in Genetics, 2014 Sperm DNA damage is associated with fertilization failure, impaired pre-and post- embryo implantation and poor pregnancy outcome. A series of methodologies to assess DNA damage in spermatozoa have been developed but most are unable to differentiate ...
Elva I eCortes-Gutierrez +5 more
doaj +1 more source
The Comet Assay: Tails of the (Un)expected. Use of the comet assay in pharmaceutical development.
Frontiers in Genetics, 2015 In genotoxicity testing of pharmaceuticals the rodent alkaline comet assay is being increasingly used as a second in vivo assay in addition to the in vivo micronucleus assay to mitigate in vitro positive results as recommended by regulatory guidance.
Bas-jan Van Der Leede
doaj +1 more source
A comparison of transgenic rodent mutation and in vivo comet assay responses for 91 chemicals. [PDF]
, 2019 A database of 91 chemicals with published data from both transgenic rodent mutation (TGR) and rodent comet assays has been compiled. The objective was to compare the sensitivity of the two assays for detecting genotoxicity.
Aardema, MJ +26 more
core +3 more sources
ATR-FTIR spectroscopy detects alterations induced by organotin(IV) carboxylates in MCF-7 cells at sub-cytotoxic/-genotoxic concentrations. [PDF]
, 2008 The environmental impact of metal complexes such as organotin(IV) compounds is of increasing concern. Genotoxic effects of organotin(IV) compounds (0.01 μg/ml, 0.1 μg/ml or 1.0 μg/ml) were measured using the alkaline single-cell gel electrophoresis ...
Ahmad, Muhammad S. +6 more
core +3 more sources
TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching. [PDF]
, 2015 The AAA+ family ATPase TRIP13 is a key regulator of meiotic recombination and the spindle assembly checkpoint, acting on signaling proteins of the conserved HORMA domain family.
Corbett, Kevin D +5 more
core +2 more sources