Results 11 to 20 of about 1,956,113 (201)

An Overview of Comet Assay Application for Detecting DNA Damage in Aquatic Animals

Agriculture, 2023
This review discusses several research studies that employed comet assay to evaluate the environmental impact of genotoxins in aquatic environments. It focuses on in vivo and in situ studies of aquatic animals.
Nan Jiang, Saima Naz, Yulin Ma, Qudrat Ullah, Muhammad Zahoor Khan, Jiaqi Wang, Xuan Lu, Dun-Zhu Luosang, Sadia Tabassum, Ahmad Manan Mustafa Chatha, Wang-Dui Basang   +10 more
doaj   +2 more sources

Measuring DNA modifications with the comet assay: a compendium of protocols. [PDF]

Nat Protoc, 2023
Collins A, Møller P, Gajski G, Vodenková S, Abdulwahed A, Anderson D, Bankoglu EE, Bonassi S, Boutet-Robinet E, Brunborg G, Chao C, Cooke MS, Costa C, Costa S, Dhawan A, de Lapuente J, Bo' CD, Dubus J, Dusinska M, Duthie SJ, Yamani NE, Engelward B, Gaivão I, Giovannelli L, Godschalk R, Guilherme S, Gutzkow KB, Habas K, Hernández A, Herrero O, Isidori M, Jha AN, Knasmüller S, Kooter IM, Koppen G, Kruszewski M, Ladeira C, Laffon B, Larramendy M, Hégarat LL, Lewies A, Lewinska A, Liwszyc GE, de Cerain AL, Manjanatha M, Marcos R, Milić M, de Andrade VM, Moretti M, Muruzabal D, Novak M, Oliveira R, Olsen AK, Owiti N, Pacheco M, Pandey AK, Pfuhler S, Pourrut B, Reisinger K, Rojas E, Rundén-Pran E, Sanz-Serrano J, Shaposhnikov S, Sipinen V, Smeets K, Stopper H, Teixeira JP, Valdiglesias V, Valverde M, van Acker F, van Schooten FJ, Vasquez M, Wentzel JF, Wnuk M, Wouters A, Žegura B, Zikmund T, Langie SAS, Azqueta A.   +78 more
europepmc   +2 more sources

Genotoxic and mutagenic properties of Ni and NiO nanoparticles investigated by comet assay, γ‐H2AX staining, Hprt mutation assay and ToxTracker reporter cell lines [PDF]

Environmental and Molecular Mutagenesis, 2017
Nickel (Ni) compounds are classified as carcinogenic to humans but the underlying mechanisms are still poorly understood. Furthermore, effects related to nanoparticles (NPs) of Ni have not been fully elucidated.
Emma Åkerlund, Francesca Cappellini, Sebastiano Di Bucchianico, S. M. Didar-Ul Islam, Sara Skoglund, Remco Derr, Inger Odnevall Wallinder, Giel Hendriks, Hanna L. Karlsson   +8 more
openalex   +2 more sources

Cell survival after DNA damage in the comet assay. [PDF]

Arch Toxicol, 2021
The comet assay is widely used in basic research, genotoxicity testing, and human biomonitoring. However, interpretation of the comet assay data might benefit from a better understanding of the future fate of a cell with DNA damage.
Bankoglu EE, Schuele C, Stopper H.
europepmc   +2 more sources

The Comet Assay as a Tool in Human Biomonitoring Studies of Environmental and Occupational Exposure to Chemicals-A Systematic Scoping Review. [PDF]

Toxics
Biomonitoring of human populations exposed to chemical substances that can act as potential mutagens or carcinogens, may enable the detection of damage and early disease prevention.
Ladeira C, Møller P, Giovannelli L, Gajski G, Haveric A, Bankoglu EE, Azqueta A, Gerić M, Stopper H, Cabêda J, Tonin FS, Collins A.   +11 more
europepmc   +2 more sources

The comet assay: ready for 30 more years [PDF]

Mutagenesis, 2017
Peter Möller
openalex   +2 more sources

Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results. [PDF]

Nat Protoc, 2020
The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems.
Møller P, Azqueta A, Boutet-Robinet E, Koppen G, Bonassi S, Milić M, Gajski G, Costa S, Teixeira JP, Costa Pereira C, Dusinska M, Godschalk R, Brunborg G, Gutzkow KB, Giovannelli L, Cooke MS, Richling E, Laffon B, Valdiglesias V, Basaran N, Del Bo' C, Zegura B, Novak M, Stopper H, Vodicka P, Vodenkova S, de Andrade VM, Sramkova M, Gabelova A, Collins A, Langie SAS.   +30 more
europepmc   +2 more sources

Comet assay: a versatile but complex tool in genotoxicity testing.

Toxicological Research, 2021
The comet assay is a versatile method for measuring DNA strand breaks in individual cells. It can also be applied to cells isolated from treated animals.
E. Cordelli, M. Bignami, F. Pacchierotti
semanticscholar   +1 more source

DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

Scientific Reports, 2021
The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations.
S. Bonassi, M. Ceppi, P. Møller, A. Azqueta, M. Milić, M. Neri, G. Brunborg, R. Godschalk, G. Koppen, S. Langie, J. Teixeira, M. Bruzzone, Juliana da Silva, D. Benedetti, D. Cavallo, C. L. Ursini, L. Giovannelli, S. Moretti, P. Riso, C. Del Bo’, P. Russo, M. Dobrzyńska, I. Goroshinskaya, E. Surikova, M. Staruchová, M. Barančoková, K. Volkovová, A. Kažimı́rová, B. Smolková, B. Laffon, V. Valdiglesias, S. Pastor, R. Marcos, Alba Hernández, G. Gajski, B. Spremo-Potparević, L. Živković, É. Boutet-Robinet, H. Perdry, P. Lebailly, C. Pérez, N. Başaran, Zsuzsanna Nemeth, A. Sáfár, M. Dusinska, A. Collins, Diana Vanessa Cristiana Costa Solange Kristine B. Carina Anderson Andrade Pereira Costa Gutzkow Ladeira Mor, Diana Anderson, Vanessa Andrade, C. Pereira, S. Costa, K. Gutzkow, C. Ladeira, M. Moretti, C. Costa, I. Orlow, E. Rojas, B. Pourrut, M. Kruszewski, S. Knasmueller, Sergey Shaposhnikov, B. Žegura, H. Stopper   +62 more
semanticscholar   +1 more source

Neutral Comet Assay to Detect and Quantitate DNA Double-Strand Breaks in Hematopoietic Stem Cells

Bio-Protocol, 2021
In vertebrates, hematopoietic stem cells (HSCs) regulate the supply of blood cells throughout the lifetime and help to maintain homeostasis. Due to their long lifespan, genetic integrity is paramount for these cells, and accordingly, a number of stem ...
Irene Roy, Pon Nadar, Satish Khurana
doaj   +1 more source