Results 11 to 20 of about 53,849 (278)

OpenComet: An automated tool for comet assay image analysis

Redox Biology, 2014
Reactive species such as free radicals are constantly generated in vivo and DNA is the most important target of oxidative stress. Oxidative DNA damage is used as a predictive biomarker to monitor the risk of development of many diseases.
Benjamin M. Gyori, Gireedhar Venkatachalam, P.S. Thiagarajan, David Hsu, Marie-Veronique Clement   +4 more
doaj   +3 more sources

Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results. [PDF]

Nat Protoc, 2020
Møller P, Azqueta A, Boutet-Robinet E, Koppen G, Bonassi S, Milić M, Gajski G, Costa S, Teixeira JP, Costa Pereira C, Dusinska M, Godschalk R, Brunborg G, Gutzkow KB, Giovannelli L, Cooke MS, Richling E, Laffon B, Valdiglesias V, Basaran N, Del Bo' C, Zegura B, Novak M, Stopper H, Vodicka P, Vodenkova S, de Andrade VM, Sramkova M, Gabelova A, Collins A, Langie SAS.   +30 more
europepmc   +2 more sources

Stochastic modeling for the COMET-assay [PDF]

, 2003
We present a stochastic model for single cell gel electrophoresis (COMET-assay) data. Essential is the use of point process structures, renewal theory and reduction to intensity histograms for further data ...
Boulesteix, Anne-Laure, Hösel, V., Liebscher, V.   +2 more
core   +3 more sources

Using a medium-throughput comet assay to evaluate the global DNA methylation status of single cells

Frontiers in Genetics, 2014
The comet assay is a simple and cost effective technique, commonly used to analyse and quantify DNA damage in individual cells. The versatility of the comet assay allows introduction of various modifications to the basic technique.
Angelique eLewies, Angelique eLewies, Etresia evan Dyk, Johannes Frederik Wentzel, Johannes Frederik Wentzel, Pieter J Pretorius   +5 more
doaj   +1 more source

Detection of DNA damage by alkaline comet assay in mouse colonic mucosa

STAR Protocols, 2021
Summary: We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin.
Elisa Boutet-Robinet, Maria M. Haykal, Saleha Hashim, Teresa Frisan, Océane C.B. Martin   +4 more
doaj   +1 more source

TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching. [PDF]

, 2015
The AAA+ family ATPase TRIP13 is a key regulator of meiotic recombination and the spindle assembly checkpoint, acting on signaling proteins of the conserved HORMA domain family.
Corbett, Kevin D, Moeller, Arne, Rosenberg, Scott C, Speir, Jeffrey A, Su, Tiffany Y, Ye, Qiaozhen   +5 more
core   +2 more sources

Interpreting sperm DNA damage in a diverse range of mammalian sperm by means of the two-tailed comet assay

Frontiers in Genetics, 2014
Sperm DNA damage is associated with fertilization failure, impaired pre-and post- embryo implantation and poor pregnancy outcome. A series of methodologies to assess DNA damage in spermatozoa have been developed but most are unable to differentiate ...
Elva I eCortes-Gutierrez, Carmen eLopez-Fernandez, Jose Luis Fernandez, Martha I Davila-Rodriguez, Stephen eJohnston, Jaime eGosalvez   +5 more
doaj   +1 more source

A comparison of transgenic rodent mutation and in vivo comet assay responses for 91 chemicals. [PDF]

, 2019
A database of 91 chemicals with published data from both transgenic rodent mutation (TGR) and rodent comet assays has been compiled. The objective was to compare the sensitivity of the two assays for detecting genotoxicity.
Aardema, MJ, Beevers, C, Bhalli, J, Douglas, GR, Escobar, PA, Farabaugh, CS, Guerard, M, Johnson, GE, Kirkland, D, Kulkarni, R, Le Curieux, F, LeBaron, MJ, Levy, DD, Long, AS, Lott, J, Lovell, DP, Luijten, M, Marchetti, F, Nicolette, JJ, Pfuhler, S, Roberts, DJ, Stankowski, LF, Thybaud, V, Weiner, SK, Williams, A, Witt, KL, Young, R   +26 more
core   +3 more sources

Automated comet assay analysis [PDF]

Cytometry, 1999
Recently the "comet assay" or "single-cell gel electrophoresis assay" has been established as a sensitive method for the detection of DNA damage and repair. Most of the software now available to quantify various parameters for DNA damage requires the interaction of a human observer. In this report, we describe an automated analysis system that is based
W, Böcker, W, Rolf, T, Bauch, W U, Müller, C, Streffer   +4 more
openaire   +2 more sources

Mutagenic impact on fish of runoff events in agricultural areas in south-west France [PDF]

, 2011
When heavy rainfall follows herbicide application, the intense surface runoff causes stream water contamination. Aquatic organisms are then briefly exposed to a complex mixture of contaminants.
Gauthier, Laury, Jean, Séverine, Laplanche, Christophe, Merlina, Georges, Pinelli, Eric, Polard, Thierry, Sanchez-Pérez, José Miguel   +6 more
core   +2 more sources