Comet assay and micronucleus (MN) test are widely applied in genotoxicity testing and biomonitoring. While comet assay permits to measure direct DNA-strand breaking capacity of a tested agent MN test allows estimating the induced amount of chromosome and/
Hovhannisyan Galina G
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Recent perspectives on the relations between faecal mutagenicity, genotoxicity and diet [PDF]
Peer reviewedPublisher ...
Hani eEl-Nezami+3 more
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Modification of comet-FISH technique by using temperature instead of chemical denaturation
Comet-FISH technique is an extension of commonly used comet assay. Its purpose is to determine whether primary DNA damage which comet assay detects occurred within a sequence of interest that is visualized by hybridization of fluorescent probe.
Marin Mladinic, Davor Zeljezic
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Elevated Level of DNA Damage and Impaired Repair of Oxidative DNA Damage in Patients with Recurrent Depressive Disorder [PDF]
Background: Depressive disorder (DD), including recurrent DD (rDD), is a severe psychological disease, which affects a large percentage of the world population.
Bielecka-Kowalska, Anna+9 more
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Closing the gap: from public concerns about health effects to science-based testing of chemicals
The comet assay is a powerful technology for measuring DNA integrity in humans and other organisms at the level of individual cells. The assay is used for research and industrial testing but suffers from a lack of standardisation, which hinders its use ...
Sergey Shaposhnikov+2 more
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Effects of Phenanthrene and Pyrene on Cytogenetic Stability of Human Dermal Fibroblasts Using Alkaline Comet Assay Technique [PDF]
In the present study, the influence of phenanthrene and pyrene on cytogenetic stability of human dermal fibroblasts using alkaline comet assay was evaluated.
Abdian, Narges.+6 more
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The colonies of C. glabrata were described, which morphology of isolated on sabouraud dextrose agar with chloramphenicol. Biosynthesis of nanoparticles is of great importance since they are highly involved in various medical and biological applications.
Teeba H. Mohammad
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Quantitation of intracellular NAD(P)H can monitor an imbalance of DNA single strand break repair in base excision repair deficient cells in real time [PDF]
DNA single strand breaks (SSBs) are one of the most frequent DNA lesions in genomic DNA generated either by oxidative stress or during the base excision repair pathways.
Asakura, Shoji+5 more
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The basic principles of DNA damage detection by the alkaline comet assay [PDF]
The Comet assay is a highly sensitive and rapid method for detecting DNA damage at the level of individual cells. It measures single-stranded and double-stranded DNA breaks, alkali-labile, incomplete excision repair and cross-linking sites.
Živković Lada+6 more
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Mutagenic impact on fish of runoff events in agricultural areas in south-west France [PDF]
When heavy rainfall follows herbicide application, the intense surface runoff causes stream water contamination. Aquatic organisms are then briefly exposed to a complex mixture of contaminants.
Gauthier, Laury+6 more
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