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Mutagenesis, 2020 As part of the safety assessment process, all industrial sectors employ genotoxicity test batteries, starting with well-established in vitro assays. However, these batteries have limited predictive capacity for the in vivo situation, which may result in ...
S. Pfuhler +10 more
semanticscholar +1 more source
Comet Assay measurements: a perspective [PDF]
Cell Biology and Toxicology, 2007 The Comet Assay or single cell gel electrophoresis assay is one of the very widely used assays to microscopically detect DNA damage at the level of a single cell. The determination of damage is carried out either through visual scoring of cells (after classification into different categories on the basis of tail length and shape) or by using different ...
Kumaravel, T. S. +3 more
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The comet assay: topical issues [PDF]
Mutagenesis, 2008 The comet assay is a versatile and sensitive method for measuring single- and double-strand breaks in DNA. The mechanism of formation of comets (under neutral or alkaline conditions) is best understood by analogy with nucleoids, in which relaxation of DNA supercoiling in a structural loop of DNA by a single DNA break releases that loop to extend into a
Lisa Giovannelli +8 more
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Potassium bromate as positive assay control for the Fpg-modified comet assay.
Mutagenesis, 2020 The comet assay is a popular assay in biomonitoring studies. DNA strand breaks (or unspecific DNA lesions) are measured using the standard comet assay.
P. Møller +22 more
semanticscholar +1 more source
Deep learning method for comet segmentation and comet assay image analysis
Scientific Reports, 2020 Comet assay is a widely used method, especially in the field of genotoxicity, to quantify and measure DNA damage visually at the level of individual cells with high sensitivity and efficiency.
Yiyu Hong +10 more
semanticscholar +1 more source
Using a medium-throughput comet assay to evaluate the global DNA methylation status of single cells
Frontiers in Genetics, 2014 The comet assay is a simple and cost effective technique, commonly used to analyse and quantify DNA damage in individual cells. The versatility of the comet assay allows introduction of various modifications to the basic technique.
Angelique eLewies +5 more
doaj +1 more source
Antioxidants and the Comet assay
Mutation Research/Reviews in Mutation Research, 2009 It is widely accepted that antioxidants, either endogenous or from the diet, play a key role in preserving health. They are able to quench radical species generated in situations of oxidative stress, either triggered by pathologies or xenobiotics, and they protect the integrity of DNA from genotoxicants.
Adolf Baumgartner +2 more
openaire +3 more sources
Frontiers in Genetics, 2014 Sperm DNA damage is associated with fertilization failure, impaired pre-and post- embryo implantation and poor pregnancy outcome. A series of methodologies to assess DNA damage in spermatozoa have been developed but most are unable to differentiate ...
Elva I eCortes-Gutierrez +5 more
doaj +1 more source
International Journal of Scientific and Technological Research, 2019 Currently, humans and other living things can have many genotoxic damages due to reasons such as chemicals, drugs, unfavorable environmental effects. Breaks in the DNA structure due to genotoxic damage can cause mutations, changes in chromosome structure and cancer.
Cücer, Nurhan +3 more
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Automated comet assay analysis [PDF]
Cytometry, 1999 Recently the "comet assay" or "single-cell gel electrophoresis assay" has been established as a sensitive method for the detection of DNA damage and repair. Most of the software now available to quantify various parameters for DNA damage requires the interaction of a human observer. In this report, we describe an automated analysis system that is based
T. Bauch +4 more
openaire +3 more sources