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Archives of Toxicology, 2021 The comet assay is a commonly used method to determine DNA damage and repair activity in many types of samples. In recent years, the use of the comet assay in human biomonitoring became highly attractive due to its various modified versions, which may be
E. Bankoglu +6 more
semanticscholar +1 more source
Detection of DNA damage by alkaline comet assay in mouse colonic mucosa
STAR Protocols, 2021 Summary: We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin.
Elisa Boutet-Robinet +4 more
doaj +1 more source
Mutagenesis, 2020 As part of the safety assessment process, all industrial sectors employ genotoxicity test batteries, starting with well-established in vitro assays. However, these batteries have limited predictive capacity for the in vivo situation, which may result in ...
S. Pfuhler +10 more
semanticscholar +1 more source
Using a medium-throughput comet assay to evaluate the global DNA methylation status of single cells
Frontiers in Genetics, 2014 The comet assay is a simple and cost effective technique, commonly used to analyse and quantify DNA damage in individual cells. The versatility of the comet assay allows introduction of various modifications to the basic technique.
Angelique eLewies +5 more
doaj +1 more source
Frontiers in Genetics, 2014 Sperm DNA damage is associated with fertilization failure, impaired pre-and post- embryo implantation and poor pregnancy outcome. A series of methodologies to assess DNA damage in spermatozoa have been developed but most are unable to differentiate ...
Elva I eCortes-Gutierrez +5 more
doaj +1 more source
Automated comet assay analysis [PDF]
Cytometry, 1999 Recently the "comet assay" or "single-cell gel electrophoresis assay" has been established as a sensitive method for the detection of DNA damage and repair. Most of the software now available to quantify various parameters for DNA damage requires the interaction of a human observer. In this report, we describe an automated analysis system that is based
W, Böcker +4 more
openaire +2 more sources
Comet Assay measurements: a perspective [PDF]
Cell Biology and Toxicology, 2007 The Comet Assay or single cell gel electrophoresis assay is one of the very widely used assays to microscopically detect DNA damage at the level of a single cell. The determination of damage is carried out either through visual scoring of cells (after classification into different categories on the basis of tail length and shape) or by using different ...
Kumaravel, T. S. +3 more
openaire +2 more sources
MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum
STAR Protocols, 2021 Summary: Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay.
Aoli Xiong +5 more
doaj +1 more source
Potassium bromate as positive assay control for the Fpg-modified comet assay.
Mutagenesis, 2020 The comet assay is a popular assay in biomonitoring studies. DNA strand breaks (or unspecific DNA lesions) are measured using the standard comet assay.
P. Møller +22 more
semanticscholar +1 more source
Is the Comet Assay a Sensitive Procedure for Detecting Genotoxicity?
Journal of Nucleic Acids, 2010 Although the Comet assay, a procedure for quantitating DNA damage in mammalian cells, is considered sensitive, it has never been ascertained that its sensitivity is higher than the sensitivity of other genotoxicity assays in mammalian cells. To determine
Satomi Kawaguchi +4 more
doaj +1 more source