Genes and Environment, 2021 Background The in vivo comet assay is used to evaluate the genotoxic potential of compounds by detecting DNA strand breaks in cells isolated from animal tissue.
Haruna Tahara +3 more
doaj +1 more source
Transcription-associated breaks in Xeroderma Pigmentosum group D cells from patients with combined features of Xeroderma Pigmentosum and Cockayne Syndrome [PDF]
, 2005 Defects in the XPD gene can result in several clinical phenotypes, including xeroderma pigmentosum (XP), trichothiodystrophy, and, less frequently, the combined phenotype of XP and Cockayne syndrome (XP-D/CS).
Andressoo, Jaan-Olle +12 more
core +3 more sources
Relationship between CpG and non-CpG DNA methylation in human lymphocytes assessed with comet assay [PDF]
The Ukrainian Biochemical JournalTissue-specific DNA methylation plays an important role in the regulation of many functional processes. The methylation level in single cells can be assessed using the comet assay (single-cell gel electrophoresis), a simple and cost-effective technique ...
M. Chopei +3 more
doaj +1 more source
Quantifying engineered nanomaterial toxicity: comparison of common cytotoxicity and gene expression measurements [PDF]
, 2017 BACKGROUND: When evaluating the toxicity of engineered nanomaterials (ENMS) it is important to use multiple bioassays based on different mechanisms of action.
Atha, Donald H. +7 more
core +2 more sources
The comet assay – from toy to tool
Frontiers in Genetics, 2015 The comet assay is nowadays the most common method for measuring DNA damage and repair in single cells. It is based on the microelectrophoretic study published by Ostling and Johanson (1984) and was developed by Singh and coworkers (1988) to a versatile ...
Guenter Speit
doaj +1 more source
Protective effects of phenolic acids on mercury-induced DNA damage in precision-cut kidney slices [PDF]
Iranian Journal of Basic Medical Sciences, 2019 Objective(s): Precision-cut tissue slices are considered an organotypic 3D model widely used in biomedical research. The comet assay is an important screening test for early genotoxicity risk assessment that is mainly applied on in vitro models. The aim
Irma Edith Carranza-Torres +7 more
doaj +1 more source
Discovery of macrocyclic inhibitors of apurinic/apyrimidinic endonuclease 1 [PDF]
, 2019 Apurinic/apyrimidinic endonuclease 1 (APE1) is an essential base excision repair enzyme that is upregulated in a number of cancers, contributes to resistance of tumors treated with DNA-alkylating or -oxidizing agents, and has recently been identified as ...
Beglov, Dmitri +12 more
core +6 more sources
Laser‐Induced Graphene from Waste Almond Shells
Advanced Functional Materials, EarlyView.Almond shells, an abundant agricultural by‐product, are repurposed to create a fully bioderived almond shell/chitosan composite (ASC) degradable in soil. ASC is converted into laser‐induced graphene (LIG) by laser scribing and proposed as a substrate for transient electronics.
Yulia Steksova +9 more
wiley +1 more source
ATM deficiency results in accumulation of DNA-Topoisomerase I covalent intermediates in neural cells [PDF]
, 2013 Accumulation of peptide-linked DNA breaks contributes to neurodegeration in humans. This is typified by defects in tyrosyl DNA phosphodiesterase 1 (TDP1) and human hereditary ataxia.
Alagoz, Meryem +3 more
core +2 more sources
Beyond Conventional Cooling: Advanced Micro/Nanostructures for Managing Extreme Heat Flux
Advanced Materials, EarlyView.This review examines the design, application, and manufacturing of biomimetic or engineered micro/nanostructures for managing high heat‐flux in multi‐level electronics by enhancing conductive, convective, phase‐changing, and radiative heat transfer mechanisms, highlighting their potential for efficient, targeted thermal management, and future prospects.
Yuankun Zhang +7 more
wiley +1 more source