Results 51 to 60 of about 1,956,113 (201)

The effect of different methods and image analyzers on the results of the in vivo comet assay

Genes and Environment, 2018
Introduction The in vivo comet assay is a widely used genotoxicity test that can detect DNA damage in a range of organs. It is included in the Organisation for Economic Co-operation and Development Guidelines for the Testing of Chemicals.
Takahiro Kyoya, Rika Iwamoto, Yuko Shimanura, Megumi Terada, Shuichi Masuda   +4 more
doaj   +1 more source

Comparing the alkaline comet with 53BP1 immunocytochemistry assay: sensitivity and cost/time effectiveness

Frontiers in Genetics, 2015
Both the comet and p53-binding protein 1 (53BP1) Immunocytochemistry (ICC) assays are well known, sensitive techniques to determine DNA damage, albeit with different perspectives.
Rajan Maynard, David Parkinson
doaj   +1 more source

Genotoxic Effects of Etoposide, Bleomycin, and Ethyl Methanesulfonate on Cultured CHO Cells: Analysis by GC-MS/MS and Comet Assay

Journal of Nucleic Acids, 2020
To evaluate methods for analysis of genotoxic effects on mammalian cell lines, we tested the effect of three common genotoxic agents on Chinese hamster ovary (CHO) cells by single-cell gel electrophoresis (comet assay) and gas chromatography-tandem mass ...
Donald H. Atha, Erdem Coskun, Onur Erdem, Alessandro Tona, Vytas Reipa, Bryant C. Nelson   +5 more
doaj   +1 more source

Investigation of Genotoxic Effect of Desflurane and Sevoflurane on Periferic Lymphocyte with Comet Assay Method

Haseki Tıp Bülteni, 2016
Aim: There are several clinical and cytogenetic studies that anesthetics cause choromosomal changes. We report data on the possible genotoxic properties of two inhalational anaesthetics, sevoflurane and desflurane in lymphocytes of children evaluated for
Yeşim Işıkçı, Ayşe Çiğdem Tütüncü, Pınar Kendigelen, Serpil Çakmakkaya, Fatiş Altındaş, Güner Kaya   +5 more
doaj   +1 more source

Evaluation of DNA damage induced by perfluorooctanoic acid using acellular, cellular, and in vivo comet assays

Zhongguo shipin weisheng zazhi, 2023
ObjectiveTo examine perfluorooctanoic acid （PFOA）-induced DNA damage using a combined in vivo and in vitro experiment.MethodsAcellular nuclear DNA damage was used as a model to test the damage induced by exposure to 0.00， 0.13， 0.25， and 0.50 mmol/L PFOA
WANG Qian, XU Tongguang, WANG Tianwei, CHEN Huihui, ZHANG Wenzhong   +4 more
doaj   +1 more source

LiCoO2 particles used in Li-ion batteries induce primary mutagenicity in lung cells via their capacity to generate hydroxyl radicals

Particle and Fibre Toxicology, 2020
Background Li-ion batteries (LIB) are used in most portable electronics. Among a wide variety of materials, LiCoO2 (LCO) is one of the most used for the cathode of LIB.
Violaine Sironval, Vittoria Scagliarini, Sivakumar Murugadoss, Maura Tomatis, Yousof Yakoub, Francesco Turci, Peter Hoet, Dominique Lison, Sybille van den Brule   +8 more
doaj   +1 more source

OpenComet: An automated tool for comet assay image analysis

Redox Biology, 2014
Reactive species such as free radicals are constantly generated in vivo and DNA is the most important target of oxidative stress. Oxidative DNA damage is used as a predictive biomarker to monitor the risk of development of many diseases.
Benjamin M. Gyori, Gireedhar Venkatachalam, P.S. Thiagarajan, David Hsu, Marie-Veronique Clement   +4 more
doaj   +1 more source

Cellular Reference Materials for DNA Damage Using Electrochemical Oxidation

Journal of Nucleic Acids, 2020
Reference materials are needed to quantify the level of DNA damage in cells, to assess sources of measurement variability and to compare results from different laboratories.
Donald H. Atha, Omobola Cole, Breece Clancy, Alessandro Tona, Vytas Reipa   +4 more
doaj   +1 more source

Genotoxicity of TiO2 nanoparticles assessed by mini-gel comet assay and micronucleus scoring with flow cytometry

Mutagenesis, 2016
The widespread production and use of nanoparticles calls for faster and more reliable methods to assess their safety. The main aim of this study was to investigate the genotoxicity of three reference TiO2 nanomaterials (NM) within the frame of the FP7 ...
S. di Bucchianico, Francesca Cappellini, Florane Le Bihanic, Yuning Zhang, K. Dreij, H. Karlsson   +5 more
semanticscholar   +1 more source

Evaluation of genotoxic activity of maleic hydrazide, ethyl methane sulfonate, and N-nitroso diethylamine in Tradescantia

Salud Pública de México, 2001
Objective. To assess the genotoxic activity of N-nitroso diethylamine (NDEA), maleic hydrazide (MH), and ethyl methane sulfonate (EMS) using two systems: the comet assay on nuclei from Tradescantia, and the pink mutation test on Tradescantia staminal ...
Alvarez-Moya Carlos, Santerre-Lucas Anne, Zúñiga-González Guillermo, Torres-Bugarín Olivia, Padilla-Camberos Eduardo, Feria-Velasco Alfredo   +5 more
doaj